| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,440,348 | T→G | 54.2% | A19A (GCT→GCG) | CJ019_03266 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,440,348 | 0 | T | G | 54.2% | ‑3.5 / 6.2 | 20 | A19A (GCT→GCG) | CJ019_03266 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (3/3); new base G (8/3); total (14/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01 | |||||||||||
ATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTG > KT2440/3440228‑3440478 | aTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCgcgcc > 7:140512/1‑135 (MQ=255)aTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCAGGCCGCGCCCCGGc > 3:28060/1‑135 (MQ=255) cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCCACGCCgat > 8:158657/1‑135 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCACCGCCTATAAGGCGCAGCGCGCGGATCAgg > 7:25957/1‑136 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCCGGCGGCCTGGGTGTGGCCGGCCACCGCCTATAAGGCGCCGCTCGCGGATCAg > 3:115886/1‑135 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCGCTGGCGGCGCCCCGGCAGCCGGGGGGTGGGAGGCCCACCCCAATAAGGCGCCGCTCGCGGAACAg > 3:90514/1‑135 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCGGCCCGGGGGTGGCAGGCCCACGCCGATAAAGAGCCGCTCGCTGCACAGGc > 1:198952/1‑135 (MQ=255) gTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCCCAGGCGGCGCCCGCGCAGCCGGGGTGTGGGAGGCCCACCCCAATAATCCGCCGCTCGCTGATCAGGCCGAtt > 8:169172/1‑134 (MQ=255) aGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCAGGCGGCGCCCCGGCCGCCGGGGGGTGGGCGGCCACCCCCCATAATGCGCCGCTCGCGGCTCAGGGCGATGACTTCGGc > 7:261460/1‑136 (MQ=255) gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGCGCCGCCGGGGGGTGGCGGGGCACCGCCGATAAGGCGCAGCTCGCGGATCACGCCGCGGACTTCGGcgaccac > 5:133802/1‑129 (MQ=255) cgggggCCCCCCGCGGGGGCGGCGGGGCGCGGCGGCGGCCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGt < 1:149296/132‑1 (MQ=255) ttGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCAGCCCGGGCGGCGCCCCCGCCGCCAGGGGGTTGGGCGCCCCCGCCGATTAGTCGGACCTCGCGGGTCATGCCGCAGACATCGGCCCGCAGTTg > 8:74410/1‑136 (MQ=255) ggggggggCGCGGCGGCGGGCGCCCCGCGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 3:177455/129‑1 (MQ=255) ggCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGGCCGGGGCGCCGGGGGGTGGCAGGCCACCCCCCATAAGGCGCAGCTCGCGGCTCAGGCCGCGGCCGGCGGCACGCCGTTGGCGGAGAAGGCTCgcgcgccccgg > 8:256503/1‑131 (MQ=255) cgcTGCTGCTGGCCGCCAGCGCTAGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTg > 7:244389/1‑110 (MQ=255) cgcTGCTGCTGGCCGCCAGCGCTAGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTg < 8:244389/110‑1 (MQ=255) cgggCCGGCTGGGCTGGCGTCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCTCACGGTCTGGAGCgg < 6:115585/133‑1 (MQ=255) ccgccCGGGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa < 7:63704/136‑1 (MQ=255) gcgctgcgcTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa < 1:23684/130‑1 (MQ=255) gcgcTGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCCACCCCGATAAGGCGCAGCTCGCTGATCAGGCCGCGGACTTAGGCAAGCAGTTGGCGGAGAAGGCTCGCGCGCCCGGTCTGGAGCGGGAGAAGGCTg > 2:152188/1‑135 (MQ=255) | ATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTG > KT2440/3440228‑3440478 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |