| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,535,852 | T→C | 8.9% | intergenic (‑73/+33) | galE ← / ← CJ019_03355 | UDP‑glucose 4‑epimerase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,535,852 | 0 | T | C | 8.9% | 135.6 / 3.0 | 45 | intergenic (‑73/+33) | galE/CJ019_03355 | UDP‑glucose 4‑epimerase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (21/20); new base C (2/2); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTGCGAGCCAATGTAGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCAGCGGCTTCAGCTGCTC > KT2440/3535734‑3535980 | tgtgCGAGCCAATGTAGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGt < 7:124939/136‑1 (MQ=255) gtgCGAGCCAATGTAGCCCGCCCCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGt < 5:64416/135‑1 (MQ=255) gtgCGAGCCAATGTAGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGt < 1:86529/135‑1 (MQ=255) gtgCGAGCCAATGTAGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGt < 4:130786/135‑1 (MQ=255) gtgCGAGCCAATGTAGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGt < 4:115404/135‑1 (MQ=255) gAGCCAATGTAGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGCCACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGAt > 3:145408/1‑135 (MQ=255) aGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGc > 2:172247/1‑135 (MQ=255) aGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGc > 5:162320/1‑135 (MQ=255) gCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGTCCATATGCTCTAt > 3:154729/1‑135 (MQ=255) gCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTAt > 5:246857/1‑135 (MQ=255) cacccacAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTAttt > 6:84170/1‑133 (MQ=255) cacccacAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTAttt > 4:217902/1‑133 (MQ=255) cacccacAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCa < 1:172247/135‑1 (MQ=255) ccacAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGaa < 8:89681/136‑1 (MQ=255) ccacAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGa < 6:247544/135‑1 (MQ=255) ccacAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCCACAGGTGACCTGTGCGTAGATGGCCATATGCTCTATTTCAAGa > 4:253573/1‑135 (MQ=255) ccacAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCGGACACGTGCCGGTGGGATGATCCGCCACAGGTGACCTGTGCGTAGCTGGCCAGATGCTCTATTTCAAGa > 4:253574/1‑135 (MQ=255) gATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAAtt < 7:234420/136‑1 (MQ=255) gATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAAtt < 8:116713/136‑1 (MQ=255) atatTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAAtt < 4:119139/135‑1 (MQ=255) atatTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAAtt < 5:122700/135‑1 (MQ=255) atatTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAAtt < 5:84170/135‑1 (MQ=255) atTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTgg > 4:141577/1‑135 (MQ=255) ccACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAg < 4:45833/134‑1 (MQ=255) aCCTCCTGGAATCTGCTGTTTACAGACGCAGGGACCACGCCCTGACCCGTGCAGGTGGGAGGATCAGCTCCAGGTGCCCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCACTTGGACATGTAGGTa < 4:244723/135‑1 (MQ=255) aaTCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAg > 3:62248/1‑135 (MQ=255) aTCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAgg < 3:141577/135‑1 (MQ=255) tCTGCTGTTTACCTACGCCGTGAACACTCCCTGACACGTGCAGGTGGGAGGATCAGCCACAGGTGACCTGTGAGTAGATGGCCATATGCTCTCTTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGt < 3:253574/135‑1 (MQ=255) tCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCCACAGGTGACCTGTGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGt < 3:253573/135‑1 (MQ=255) ctgTTTACAGACGCAGTGCACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATgg > 8:82365/1‑136 (MQ=255) ctgTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATgg > 7:6169/1‑136 (MQ=255) aCAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTAt > 2:257485/1‑135 (MQ=255) tCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTAcc > 8:268425/1‑99 (MQ=255) tCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTAcc < 7:268425/99‑1 (MQ=255) ccTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCAc > 8:210839/1‑136 (MQ=255) tGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCAcc > 1:178204/1‑135 (MQ=255) acGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCa > 2:162613/1‑135 (MQ=255) acGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCa > 2:8819/1‑135 (MQ=255) cAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCCGCGGc > 5:39164/1‑135 (MQ=255) cAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCAGCGGc > 2:22333/1‑135 (MQ=255) gTGGGATTATCAGCTACAGGTGACATGATCGTAGATGTCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCAGCGGCTTc < 2:41807/135‑1 (MQ=255) gggATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCAGCGGCTTCAg < 3:216301/135‑1 (MQ=255) gatCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCAGCGGCTTCAGCTGCTc > 8:67256/1‑136 (MQ=255) atCAGCTACAGGTGACCTGAGCGTAGATGGCCata < 1:226439/35‑1 (MQ=255) atCAGCTACAGGTGACCTGAGCGTAGATGGCCata > 2:226439/1‑35 (MQ=255) | TGTGCGAGCCAATGTAGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCAGCGGCTTCAGCTGCTC > KT2440/3535734‑3535980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |