Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,535,865 A→T 8.2% intergenic (‑86/+20) galE ← / ← CJ019_03355 UDP‑glucose 4‑epimerase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,535,8650AT 8.2% 146.3 / 4.2 49intergenic (‑86/+20)galE/CJ019_03355UDP‑glucose 4‑epimerase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (24/21);  new base T (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

TGTGCGAGCCAATGTAGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCAGCGGCTTCAGCTGCTCCCGTGCATCGCGGGCGAA  >  KT2440/3535734‑3535998
                                                                                                                                   |                                                                                                                                     
tgtgCGAGCCAATGTAGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGt                                                                                                                                   <  7:124939/136‑1 (MQ=255)
 gtgCGAGCCAATGTAGCCCGCCCCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGt                                                                                                                                   <  5:64416/135‑1 (MQ=255)
 gtgCGAGCCAATGTAGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGt                                                                                                                                   <  4:130786/135‑1 (MQ=255)
 gtgCGAGCCAATGTAGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGt                                                                                                                                   <  1:86529/135‑1 (MQ=255)
 gtgCGAGCCAATGTAGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGt                                                                                                                                   <  4:115404/135‑1 (MQ=255)
     gAGCCAATGTAGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGCCACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGAt                                                                                                                               >  3:145408/1‑135 (MQ=255)
               aGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGc                                                                                                                     >  2:172247/1‑135 (MQ=255)
               aGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGc                                                                                                                     >  5:162320/1‑135 (MQ=255)
                    gCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGTCCATATGCTCTAt                                                                                                                >  3:154729/1‑135 (MQ=255)
                    gCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTAt                                                                                                                >  5:246857/1‑135 (MQ=255)
                        cacccacAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTAttt                                                                                                              >  4:217902/1‑133 (MQ=255)
                        cacccacAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTAttt                                                                                                              >  6:84170/1‑133 (MQ=255)
                        cacccacAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCa                                                                                                            <  1:172247/135‑1 (MQ=255)
                           ccacAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGaa                                                                                                        <  8:89681/136‑1 (MQ=255)
                           ccacAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGa                                                                                                         <  6:247544/135‑1 (MQ=255)
                           ccacAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCCACAGGTGACCTGTGCGTAGATGGCCATATGCTCTATTTCAAGa                                                                                                         >  4:253573/1‑135 (MQ=255)
                           ccacAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCGGACACGTGCCGGTGGGATGATCCGCCACAGGTGACCTGTGCGTAGCTGGCCAGATGCTCTATTTCAAGa                                                                                                         >  4:253574/1‑135 (MQ=255)
                                    gATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAAtt                                                                                               <  7:234420/136‑1 (MQ=255)
                                    gATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAAtt                                                                                               <  8:116713/136‑1 (MQ=255)
                                     atatTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAAtt                                                                                               <  5:84170/135‑1 (MQ=255)
                                     atatTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAAtt                                                                                               <  5:122700/135‑1 (MQ=255)
                                     atatTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAAtt                                                                                               <  4:119139/135‑1 (MQ=255)
                                       atTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTgg                                                                                             >  4:141577/1‑135 (MQ=255)
                                                ccACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAg                                                                                     <  4:45833/134‑1 (MQ=255)
                                                  aCCTCCTGGAATCTGCTGTTTACAGACGCAGGGACCACGCCCTGACCCGTGCAGGTGGGAGGATCAGCTCCAGGTGCCCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCACTTGGACATGTAGGTa                                                                                  <  4:244723/135‑1 (MQ=255)
                                                           aaTCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAg                                                                         >  3:62248/1‑135 (MQ=255)
                                                            aTCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAgg                                                                        <  3:141577/135‑1 (MQ=255)
                                                             tCTGCTGTTTACCTACGCCGTGAACACTCCCTGACACGTGCAGGTGGGAGGATCAGCCACAGGTGACCTGTGAGTAGATGGCCATATGCTCTCTTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGt                                                                       <  3:253574/135‑1 (MQ=255)
                                                             tCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCCACAGGTGACCTGTGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGt                                                                       <  3:253573/135‑1 (MQ=255)
                                                                 ctgTTTACAGACGCAGTGCACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATgg                                                                  >  8:82365/1‑136 (MQ=255)
                                                                 ctgTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATgg                                                                  >  7:6169/1‑136 (MQ=255)
                                                                       aCAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTAt                                                             >  2:257485/1‑135 (MQ=255)
                                                                                        tCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTAcc                                                                                >  8:268425/1‑99 (MQ=255)
                                                                                        tCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTAcc                                                                                <  7:268425/99‑1 (MQ=255)
                                                                                          ccTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCAc                                         >  8:210839/1‑136 (MQ=255)
                                                                                            tGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCAcc                                        >  1:178204/1‑135 (MQ=255)
                                                                                                acGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCa                                    >  2:162613/1‑135 (MQ=255)
                                                                                                acGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCa                                    >  2:8819/1‑135 (MQ=255)
                                                                                                     cAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCCGCGGc                               >  5:39164/1‑135 (MQ=255)
                                                                                                     cAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCAGCGGc                               >  2:22333/1‑135 (MQ=255)
                                                                                                        gTGGGATTATCAGCTACAGGTGACATGATCGTAGATGTCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCAGCGGCTTc                            <  2:41807/135‑1 (MQ=255)
                                                                                                          gggATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCAGCGGCTTCAg                          <  3:216301/135‑1 (MQ=255)
                                                                                                               gatCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCAGCGGCTTCAGCTGCTc                    >  8:67256/1‑136 (MQ=255)
                                                                                                                atCAGCTACAGGTGACCTGAGCGTAGATGGCCata                                                                                                                        >  2:226439/1‑35 (MQ=255)
                                                                                                                atCAGCTACAGGTGACCTGAGCGTAGATGGCCata                                                                                                                        <  1:226439/35‑1 (MQ=255)
                                                                                                                               ccTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCAGCGGCTTCAGCTGCTCCCGTGCATCGCGGGc     <  4:145408/135‑1 (MQ=255)
                                                                                                                                 tGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCAGCGGCTTCAGCTGCTCCCGTGCATCGCGGGCGa   >  6:10307/1‑135 (MQ=255)
                                                                                                                                  gAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTCGGCCACCGGCAGCGGCTTCAGCTGCTCCCGTGCATCGCGGGCGaa  >  5:149531/1‑135 (MQ=255)
                                                                                                                                  gAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCCCCGGCAGCGGCGTCAGCTGCTCCCGTGCCTCGCGGGCGaa  >  5:152890/1‑135 (MQ=255)
                                                                                                                                   |                                                                                                                                     
TGTGCGAGCCAATGTAGCCCGCACCACCCACAACCAGATATTTCATCCCCACCTCCTGGAATCTGCTGTTTACAGACGCAGTGAACACTCCCTGACACGTGCAGGTGGGATGATCAGCTACAGGTGACCTGAGCGTAGATGGCCATATGCTCTATTTCAAGAATAGCCAATTGGACATGTAGGTACCGTCGAAGGTAATGGTGTATTGCCCATCCTTGTAGGCCACCGGCAGCGGCTTCAGCTGCTCCCGTGCATCGCGGGCGAA  >  KT2440/3535734‑3535998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑