Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,957,251 G→A 100% P133L (CCG→CTG)  dmlR_13 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,957,2510GA100.0% 332.3 / ‑6.8 92P133L (CCG→CTG) dmlR_13HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (40/51);  minor base T (0/1);  total (40/52)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGC  >  KT2440/3957119‑3957382
                                                                                                                                    |                                                                                                                                   
acacCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCAAGCCCTTCGGCCAGCagg                                                                                                                                   <  3:255183/135‑1 (MQ=255)
       gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGt                                                                                                                            >  1:175574/1‑135 (MQ=255)
       gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGt                                                                                                                            >  5:51252/1‑135 (MQ=255)
       gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGt                                                                                                                            >  4:134433/1‑135 (MQ=255)
       gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGGGATCACCACGTCATGCCCTGCGGCCAGCAGGTCTGGGtt                                                                                                                           >  7:33466/1‑136 (MQ=255)
         gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTgc                                                                                                                         >  7:209436/1‑136 (MQ=255)
         gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTgc                                                                                                                         >  8:60646/1‑136 (MQ=255)
         gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTgc                                                                                                                         >  7:249107/1‑136 (MQ=255)
         gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTgc                                                                                                                         >  8:3189/1‑136 (MQ=255)
         gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                          >  3:221045/1‑135 (MQ=255)
         gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                          >  6:5328/1‑135 (MQ=255)
         gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTg                                                                                                                          >  2:153337/1‑135 (MQ=255)
         gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGGTg                                                                                                                          >  5:272489/1‑135 (MQ=255)
         gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCAAGTCATGCCATTCGGCCAGCAGGTCGGGGTTg                                                                                                                          >  5:197379/1‑135 (MQ=255)
              cAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTg                                                                                                                     <  1:104490/135‑1 (MQ=255)
              cAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTg                                                                                                                     <  6:58394/135‑1 (MQ=255)
                     ccGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGc                                                                                                              <  4:115214/135‑1 (MQ=255)
                     ccGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGc                                                                                                              >  4:32358/1‑135 (MQ=255)
                      cGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCg                                                                                                             >  5:260090/1‑135 (MQ=255)
                       gggggCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACCGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCCCCACGTCATGCCCTTCGGCCAGCAGGGCGGGGTTGCGCTGCGACAGCgg                                                                                                            >  5:179952/1‑134 (MQ=255)
                        ggggCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGt                                                                                                            >  8:227219/1‑134 (MQ=255)
                        ggggCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTc                                                                                                           <  5:83955/135‑1 (MQ=255)
                         gggCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCa                                                                                                          >  6:2449/1‑135 (MQ=255)
                         gggCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCa                                                                                                          <  6:172248/135‑1 (MQ=255)
                           gCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGc                                                                                                        <  3:133978/135‑1 (MQ=255)
                             cgcgCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTc                                                                                                      <  1:153337/135‑1 (MQ=255)
                             cgcgCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTc                                                                                                      <  6:195926/135‑1 (MQ=255)
                              gcgcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGa                                                                                                    <  8:109651/136‑1 (MQ=255)
                               cgcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGAt                                                                                                   <  8:63065/136‑1 (MQ=255)
                                gcAGGGTATGCTGAAAATATTGCCCAGCGTCACAGAGATCAGTTTCGAATCCGGCAGCGCACCTTCCAGGGTGATCACCACGTCATGCCCTTAGGCCAGCAGTTCGGGGTTGCGCTGCGACATCGTCAGCTCGAt                                                                                                   <  6:197379/135‑1 (MQ=255)
                                    gggATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGTCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCTGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATcacca                                                                                              <  8:230964/136‑1 (MQ=255)
                                     ggATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCGGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATcacca                                                                                              <  4:185567/135‑1 (MQ=255)
                                     ggATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATcacca                                                                                              <  2:36421/135‑1 (MQ=255)
                                      gATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATcaccac                                                                                             <  6:220104/135‑1 (MQ=255)
                                            gAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCACGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCCCTTCCgg                                                                                       >  4:155616/1‑135 (MQ=255)
                                               aaTATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTaa                                                                                   <  7:66787/136‑1 (MQ=255)
                                               aaTATTGCCCAGCGACACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTa                                                                                    <  2:80070/135‑1 (MQ=255)
                                                 tatTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGt                                                                                     >  4:131864/1‑132 (MQ=255)
                                                 tatTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTaaa                                                                                  >  5:221006/1‑135 (MQ=255)
                                                     gCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTg                                                                                                                     >  3:42808/1‑96 (MQ=255)
                                                     gCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTg                                                                                                                     <  4:42808/96‑1 (MQ=255)
                                                      cccAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCAtg                                                                             >  4:168600/1‑135 (MQ=255)
                                                                      tCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTgc                                                                                                                         <  4:114126/75‑1 (MQ=255)
                                                                      tCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTgc                                                                                                                         >  3:114126/1‑75 (MQ=255)
                                                                        aGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGc                                                                                                        >  2:231928/1‑90 (MQ=255)
                                                                        aGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGc                                                                                                        <  1:231928/90‑1 (MQ=255)
                                                                        aGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGccacca                                                             <  4:129688/133‑1 (MQ=255)
                                                                        aGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGccacca                                                             >  3:129688/1‑133 (MQ=255)
                                                                         gTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGc                                                           <  4:81760/134‑1 (MQ=255)
                                                                           tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCCTGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                                        <  4:221045/135‑1 (MQ=255)
                                                                               aaTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                   <  8:249107/136‑1 (MQ=255)
                                                                               aaTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                   <  7:151754/136‑1 (MQ=255)
                                                                                aTCCGGCATCGCACCGTCGACGGTGATCAACACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCTCTGCGACAGCGTCAGCTCGATCACCACTTCCTTGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                   <  6:91104/135‑1 (MQ=255)
                                                                                aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                   <  3:146591/135‑1 (MQ=255)
                                                                                aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                   <  1:225488/135‑1 (MQ=255)
                                                                                aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                   <  3:111300/135‑1 (MQ=255)
                                                                                aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                   <  4:157800/135‑1 (MQ=255)
                                                                                aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                   <  6:74028/135‑1 (MQ=255)
                                                                                aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTGCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                   <  5:254847/135‑1 (MQ=255)
                                                                                aTCCGGCAGCGCACCGTCCACGGTCATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                                   <  5:65877/135‑1 (MQ=255)
                                                                                    ggCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAAc                                               <  3:93546/135‑1 (MQ=255)
                                                                                         cgcACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGccc                                          <  4:76494/135‑1 (MQ=255)
                                                                                           cACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCa                                        <  4:140100/135‑1 (MQ=255)
                                                                                                 ccACGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGt                                  >  3:114195/1‑135 (MQ=255)
                                                                                                   aCGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCGCCATGCCGTGc                                >  4:250822/1‑135 (MQ=255)
                                                                                                   aCGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGc                                <  4:269697/135‑1 (MQ=255)
                                                                                                    cGGTGATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                               <  3:131864/135‑1 (MQ=255)
                                                                                                        gATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCa                           >  2:3165/1‑135 (MQ=255)
                                                                                                        gATCACCACGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACATCCGGCTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCACCAGCCCCATGCCGTGCGGGCa                           >  3:217047/1‑135 (MQ=255)
                                                                                                           caccacGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGt                         >  3:46623/1‑134 (MQ=255)
                                                                                                             ccacGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGccac                                                               <  1:93475/94‑1 (MQ=255)
                                                                                                             ccacGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGccac                                                               >  2:93475/1‑94 (MQ=255)
                                                                                                             ccacGTCATGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGc                       >  5:128495/1‑134 (MQ=255)
                                                                                                                    aTGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                               >  4:260630/1‑119 (MQ=255)
                                                                                                                    aTGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                               <  3:260630/119‑1 (MQ=255)
                                                                                                                     tGCCCTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGAt             <  8:64801/136‑1 (MQ=255)
                                                                                                                        ccTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATgg           >  2:57978/1‑135 (MQ=255)
                                                                                                                        ccTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATgg           >  2:48667/1‑135 (MQ=255)
                                                                                                                        ccTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc          <  7:175969/136‑1 (MQ=255)
                                                                                                                        ccTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc          >  8:175969/1‑136 (MQ=255)
                                                                                                                         cTTCGGCCAGCTGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc          <  5:178021/135‑1 (MQ=255)
                                                                                                                         cTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc          <  2:88413/135‑1 (MQ=255)
                                                                                                                         cTTCGGCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc          <  1:57978/135‑1 (MQ=255)
                                                                                                                         cTTCGGCCAGCAGGTCGGGGTTGCGCGGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc          <  1:192016/135‑1 (MQ=255)
                                                                                                                         cTTCGGCCAGCAGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCCCCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGc          <  5:5328/135‑1 (MQ=255)
                                                                                                                              gCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGc                                                                                                        >  7:273013/1‑36 (MQ=255)
                                                                                                                              gCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGc                                                                                                        <  8:273013/36‑1 (MQ=255)
                                                                                                                              gCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTa                                                                                    <  7:234531/56‑1 (MQ=255)
                                                                                                                              gCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTa                                                                                    >  8:234531/1‑56 (MQ=255)
                                                                                                                              gCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAg    <  8:37742/136‑1 (MQ=255)
                                                                                                                              gCCAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGAACCAGACCCATGAAGTGCGGGCAGTGCAAACGCAGATGGCCGCGa     >  1:257797/1‑135 (MQ=255)
                                                                                                                               ccAGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAg    <  1:185965/135‑1 (MQ=255)
                                                                                                                                cagcagGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAgg   <  1:3165/135‑1 (MQ=255)
                                                                                                                                 aGCAGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGc  <  6:221006/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
ACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGC  >  KT2440/3957119‑3957382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑