| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,135,607 | A→C | 49.9% | Q32P (CAG→CCG) | tgnC → | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,135,607 | 0 | A | C | 49.9% | 0.5 / 15.5 | 30 | Q32P (CAG→CCG) | tgnC | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (5/9); new base C (11/4); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01 | |||||||||||
AAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTC > KT2440/4135473‑4135738 | aaGCCCGCACCCTCCCCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCa < 3:192504/135‑1 (MQ=255)aaGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCa < 3:200244/135‑1 (MQ=255) cccGCACCCTCACCGAATCCGCCAAGGGGATTCTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGAAGGCCAATGGCGCGATGCCCAGAGAGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCAgc > 4:263760/1‑133 (MQ=255) cGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGCCCt > 1:25553/1‑135 (MQ=255) ccGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGCCAGCCTCACCCCGGCCACCGCCCCGGCCAGGGCGCCCCAGCCCGAc > 7:127867/1‑136 (MQ=255) ccGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGCCAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGAc > 8:74471/1‑136 (MQ=255) gCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGCCCGGGGCGCACCAGCCcgccgc > 7:30317/1‑136 (MQ=255) cAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGCCGGCCAATGGCGCGATGCCCAGAGCGGCGAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCACTGCCCgccgcc > 3:179160/1‑135 (MQ=255) cggggATTGTTGCATCACCCTCGGTCGTTGCCCGATGTGCATCGACGGCCAATGGCGCGATGCCCAGCGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGa < 6:182437/134‑1 (MQ=255) ggtttgttgCATGACCCTGGTTCGTTTGCAGATGTGCTTCGACGGCCATTGGCGCTAGGCCCAGCGCGGCAAGACCGGCGACTGCCTCAACCCGGCCACCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGa < 4:54031/132‑1 (MQ=255) ggATTGTTCCATGACCATCGTGCGTTTCCATTTGGCAATCGACGGCCATTGGCGCGATGCCAAGCGCGCAAAGACCTTCGACCGCCTCAACCCGCCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGa < 6:3582/135‑1 (MQ=255) gATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCCCCTGCCCGCCGCCGAGGAAg > 7:195442/1‑136 (MQ=255) ttgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCCCCTGCCCGCCGCCGCTGCAGCCgc > 3:216176/1‑134 (MQ=255) cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCGGCCCCGGCGGCGCTGCCCGCCCCCGCGGAAGCCGAAGTggt > 1:272136/1‑132 (MQ=255) cccTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCCCCCCGGGCCCAACTGCCCGACGCCGCGGAAGCGGCTGTGGAGc > 5:223644/1‑135 (MQ=255) cAGATGTCCATCCACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGgcc < 5:77921/135‑1 (MQ=255) cagcgccGGCCAAGGGCGCGAGGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGt < 8:195442/130‑1 (MQ=255) gACGGCCAAGGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCACCCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGcc < 6:46632/135‑1 (MQ=255) aTGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCCACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGAccg > 8:267634/1‑133 (MQ=255) gcgcGAGGCCCAGCCCGCCAAGACCTGCCACCGCCTCAACCCGCCCCCCGCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAg < 7:25154/136‑1 (MQ=255) agagCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGGGCAACTGCCCGCCGCCGAGGAAGCCGATGTGGCGCTGGCCGTTCCGGCCGCCCCGCCGGCCATCGCCCGCCAGGCCTGgcgcc > 6:258185/1‑134 (MQ=255) gagCGCCAAGACCTGCGACAGCCGCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCgcag < 6:65010/135‑1 (MQ=255) aGACCTTCGACAGCCTCAACCCGGCCCCCGCCCAGGCCTGGGCGCACCTGCCCGACGCCGCTGGAGCCGATGTGGCGCTGGCCGTTCAGGCCGCCCAGCGTGCCGTCGACAGCAAGGCGTGGCGCAGCATCACCg > 4:210053/1‑135 (MQ=255) gACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCTGGGCGCCACAGCCCGCCCCCGCTGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGAGCCTTCGACAGCAAGTCCTGGCGCAGCATCAccc > 3:28689/1‑133 (MQ=255) gCCTCACCCCGGCCCCCGCCCAGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGGGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 4:235291/135‑1 (MQ=255) cccTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 3:38952/134‑1 (MQ=255) ccTCACCCCGGCCCCCGCCCCGGCGGGGGCGCAACTGCCCGACGCCGAGGCAGCCGATGTGGAGCTGGCCGTTCCGGCCGGCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 7:262016/134‑1 (MQ=255) cAACCCGGCCACCGCCCACGCCGGGGCGCCACTGCCCGACGCCGATGAAGCCGCTGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTGGACACCCAGGCGGGGCGCCGCATCACCGCGACCGCCCGCGGcca > 2:57145/1‑133 (MQ=255) cGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctgct < 8:127867/136‑1 (MQ=255) ggCCACCGCCCGGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAgctgct < 4:236288/135‑1 (MQ=255) ccccGCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGcgt < 7:236422/134‑1 (MQ=255) aCCGCCCCGGCCGGGGCGGCCCTGCCCGCCCCCGCGGGAGCCGATGTGGAGCTGGCCGGTCAGGCCGCCCCCCGTGGCGTCGACAGCAACGCCTGGCGCCGCCTCCCCCCGACCGCCCCCGGCCAGCTGCTGcgtc > 8:25279/1‑136 (MQ=255) ccccGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCgtcgtc < 5:251980/135‑1 (MQ=255) | AAGCCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTCGTC > KT2440/4135473‑4135738 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |