Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,165,443	T→G	13.0%	N109H (AAC→CAC)	ribN ←	Riboflavin transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,165,443	0	T	G	13.0%	150.5 / 4.4	67	N109H (AAC→CAC)	ribN	Riboflavin transporter
Reads supporting (aligned to +/- strand):  ref base T (35/20);  new base G (2/8);  total (37/30)
Fisher's exact test for biased strand distribution p-value = 1.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCCAGGGTGCACCACCACCAGCACGCCGATAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCAATACCGGCCGGCGGGTGCGCAACACGTTGAGCCCAGACTT  >  KT2440/4165316‑4165575                                                                                                                                |                                                                                                                                     ccGCCAGGGTGCACCACCACCAGCACGCCGATAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGcc                                                                                                                               >  5:62474/1‑135 (MQ=255) ccGCCAGGGTGCACCACCACCAGCACGCCGATAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGcc                                                                                                                               >  4:212866/1‑135 (MQ=255) ccGCCAGGGTGCACCACCACCAGCACGCCGATAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGcc                                                                                                                               >  6:143497/1‑135 (MQ=255) ccGCCAGGGTGCACCACCACCAGCACGCCGATAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGcc                                                                                                                               >  3:138110/1‑135 (MQ=255) ccGCCAGGGTGCACCACCACCAGCACGCCGATAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGcc                                                                                                                               >  2:41015/1‑135 (MQ=255) ccGCCAGGGTGCACCACCACCAGCACGCCGATAAACCCCATCACCACCCCCACCCACTGCCCCACAGTCACTCGCGCTTTGACCAGCGGCCCCGACAGCGCGGTGACCAGCACCCTGGCCAGGAAGTTGaacgcc                                                                                                                               >  2:253642/1‑130 (MQ=255)  cGCCAGGGTGCACCACCACCAGCACGCCGATAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGCCAGCGCGGTGACCAGCCCCGGGGCAAGGAAGTTGCCGGCCGt                                                                                                                             >  8:170747/1‑136 (MQ=255)  cGCCAGGGTGCACCACCACCAGCACGCCGATAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCg                                                                                                                              >  6:223079/1‑135 (MQ=255)       gggTGCACCACCACCAGCACGCCGATAAAGCCCATCACCACCGCCCCCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGCCCAGCCCCGGGGCAAGGAAGTTGACGGCCGTGGcc                                                                                                                         >  1:251057/1‑135 (MQ=255)       gggTGCACCACCACCAGCACGCCGATAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGcc                                                                                                                         >  2:236254/1‑135 (MQ=255)         gggCACCACCACCAGCACCCCGATACACCCCAGCCCCACCGCCCCCCACTGCCCCACTGACACGCGCTCTTTGCGCAGCGGCGCCGACTGCGCGGGGACCAGCACCGGGGCAAGGAAGTGGACGGCCGTGGCCTc                                                                                                                       <  4:81050/133‑1 (MQ=255)         ggccaccaccccCCCCCCCCCCATAACCCCCACCACCCCCCCCACCCCCTGCCCCACTGTCACTCGCTCTTTGCGCAGCGGCGCCGACCGCGCGGGGACCAGCACCGGGGCAAGGAAGTTCACGGCCGTGGCCTc                                                                                                                       <  5:223079/132‑1 (MQ=255)            caccaccacCAGCACGCCGATAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGCCAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCGCGGc                                                                                                                    >  6:211069/1‑135 (MQ=255)               cacccccaGCACGCCGAAAAAGCCCATCACCACCGCCACCCACTGCCCCACTGGCACTCGCTCTTTGCGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGt                                                                                                                <  7:51702/136‑1 (MQ=255)                   ccccccACCCCGAAAAAGCCCACCACCCCCCCCACCCACTCCCCCACTGTCACGCGCTCTTTCCGCCGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGc                                                                                                             <  1:50939/130‑1 (MQ=255)                     caGCACGCCGATAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAg                                                                                                           >  4:82695/1‑135 (MQ=255)                      aGCACGCCGATAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAgg                                                                                                          >  5:1099/1‑135 (MQ=255)                       gCACGCCGATAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGt                                                                                                         >  3:49314/1‑135 (MQ=255)                       gCACGCCGATAAAGCCCATAACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGt                                                                                                         >  2:54703/1‑135 (MQ=255)                               aTAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGCCAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAg                                                                                                 >  4:48031/1‑135 (MQ=255)                               aTAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAg                                                                                                 >  4:233320/1‑135 (MQ=255)                                    gCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGGGGTGACCAGCACCGGGGAAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTc                                                                                           <  7:7456/136‑1 (MQ=255)                                      ccatacccacgGCCACCCACTGCCACTCTGTATCTAGCTTTTTGCGCAGCGGCGCGGACAGCGGGGGGACCAGCACCGGGGCTAGCAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGt                                                                                         <  8:244983/130‑1 (MQ=255)                                          caccacCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGGGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGaa                                                                                      <  6:116115/135‑1 (MQ=255)                                          caccacCGCCACCCACAGCCCCACTGTCACTCGCACTTTGAGCAGCCGCGCCCACAGCGCGGTGACCAGCACCCGGGCAAGGAAGTTGACAGCCGTCGCCTCGGCCACTGCCAGGTAATGCACGCCGGTGGTGaa                                                                                      >  2:103263/1‑135 (MQ=255)                                            ccaccGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAAca                                                                                    >  1:9794/1‑135 (MQ=255)                                            ccaccGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTGGACTGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAAca                                                                                    >  3:172520/1‑135 (MQ=255)                                            ccaccGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTGGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAAca                                                                                    >  3:175924/1‑135 (MQ=255)                                               tccacacccccCGCCCCACTGTCACGCGCTCTTGGCGCCGCGGCGCCGACCGGGGGGTGACCACCCCGGGGGAAAGTATGTGCACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAAcagca                                                                                 <  5:11502/131‑1 (MQ=255)                                                  ccacccacTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGTAcaccac                                                                                >  6:249900/1‑132 (MQ=255)                                                  ccacccacTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGc                                                                              >  5:43407/1‑135 (MQ=255)                                                    ccccacGCCCCCACTGTCACTCGCTCTTGGGGCAGCGGCGCCGACTGCGCGGTGACCAGCCCGGGGGCATGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTg                                                                            <  6:62474/134‑1 (MQ=255)                                                         cTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCt                                                                       <  2:187037/135‑1 (MQ=255)                                                             cccACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGCACAGCAGGCGGGTGGTca                                                                     >  6:144493/1‑133 (MQ=255)                                                             cccACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTcag                                                                    >  7:208754/1‑134 (MQ=255)                                                             cccACTCGCACTCGCTCTTCGCGCCGCGGCGCCGACCGCGCGGTGACCAGCACCGGGGCAAGGACGGGCACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTca                                                                     <  1:108343/133‑1 (MQ=255)                                                               cACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGGATTGCAAGCCGGTCGTGGACCGCCGGCGGGTGGTcagccg                                                                 >  5:174372/1‑133 (MQ=255)                                                                 cTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGACCCGCCGGCGGGTGCTCAGCCggg                                                               >  6:241176/1‑134 (MQ=255)                                                                  tgacACTCGCTCTTTGAGCAGCGGGGCCGACAGCGGGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCt                                                              <  4:172520/132‑1 (MQ=255)                                                                  tGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACCGCAGGCGGGTGCGCAGCCGGcg                                                              >  1:172638/1‑134 (MQ=255)                                                                  tGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTGGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCt                                                              <  4:175924/135‑1 (MQ=255)                                                                     cACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGCACAGCCGGCGGGTGGTCAGCCGGCTCAgg                                                          >  7:76268/1‑136 (MQ=255)                                                                         cGCTCTTTGCGCAGCGGCGCCTCACGCGCGGTGACCAGCAGCGGGGCAAGGAAGTCGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGccc                                                      <  7:118262/136‑1 (MQ=255)                                                                         cGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGACCAGCAGGCTGGTGCTCAGCCGGCGCAGGGcc                                                       >  6:82101/1‑135 (MQ=255)                                                                          gCTCTTTGAGCAGCGGCGCGGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGccc                                                      <  1:54703/135‑1 (MQ=255)                                                                          gCTCTTTGAGCAGCGGCGCCGACAGCGGGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGccc                                                      <  2:55466/135‑1 (MQ=255)                                                                          gCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGccc                                                      <  3:233320/135‑1 (MQ=255)                                                                                  agcagcGGCGCCGACAGCGCGGTGACCTCCACGGGGTCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGt                                               <  6:115898/134‑1 (MQ=255)                                                                                       cggcgCCGCCCGCGCGGGGACCACCACCGGGGCAAGGAAGGGGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCaa                                         <  3:212866/135‑1 (MQ=255)                                                                                       cggcgCCGACCGCGCGGGCACCCGCCCCGGGGCAAGGAAGGTGACGGCCGGGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCaa                                         <  5:66387/135‑1 (MQ=255)                                                                                         gcgcCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCCATAc                                      >  7:158538/1‑136 (MQ=255)                                                                                         gcgcCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCAATa                                       >  1:66089/1‑135 (MQ=255)                                                                                                 aGCGCGGTGACCAGCACCGGGGCAAGGACGTGGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCAATAccggccgg                               <  6:113862/135‑1 (MQ=255)                                                                                                   ggcgGTGACCACCCCGGGGGCAAGGACGTGGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCAATACCGGCcggcg                             <  3:248546/134‑1 (MQ=255)                                                                                                              gCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCAATACCGGCCGGCGGGTGCGCAaca                  <  4:49314/135‑1 (MQ=255)                                                                                                               ccccGGGGCATGTAAGTCGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCAATACCGGCCGGCGGGTGCGCAacac                 <  5:249900/133‑1 (MQ=255)                                                                                                               cACGGGGCCAAGGACGTTGACGGCCGGGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCAATACCGGCCGGCGGGTGCGCAacac                 <  3:47823/135‑1 (MQ=255)                                                                                                               cACCGGGGCATGGACGTGGACGGCCGTGGCCTCGGCCAGTGGCAGGGATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCAATACCGGCCGGCGGGTGCGCAacac                 <  1:249679/135‑1 (MQ=255)                                                                                                               cACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCAATACCGGCCGGCGGGTGCGCAacac                 <  1:236254/135‑1 (MQ=255)                                                                                                                       cAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGCACAGCAGGCTGGTGCTCAGCCGGCTCAGGGCCCGCAAGGTCTGCAATACCGGCCGGCGGGTGCGCAACACGTTGCGcc         >  6:131661/1‑135 (MQ=255)                                                                                                                       cAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCATGGCCCGCAATCTACGCAATACCGGCCGGCCTGTGGGCAACAAGTTGATccc        >  8:58501/1‑136 (MQ=255)                                                                                                                       cAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCAATACCGGCCGGCGGGTGCGCAACACGTTGAGcc         >  6:185874/1‑135 (MQ=255)                                                                                                                         aGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCAATACCGGCCGGCGGGTGCGCAACACGTTGAGCCCa       <  2:9794/135‑1 (MQ=255)                                                                                                                            aaGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGCACAGCAGGCTGGTGCTCAGCCGGCGCAGGGCCCGCAAGGTCTGCAATACCGGCCGGCGGGTGGGGAACACGTTGCGCCCAGCCt   >  7:211778/1‑136 (MQ=255)                                                                                                                            aaGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAggg                                                         >  6:64558/1‑82 (MQ=255)                                                                                                                            aaGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAggg                                                         <  5:64558/82‑1 (MQ=255)                                                                                                                              gTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCAATACCGGCCGGCGGGTGCGCAACACGTTGAGCCCAGACtt  <  3:82695/135‑1 (MQ=255)                                                                                                                                |                                                                                                                                     CCGCCAGGGTGCACCACCACCAGCACGCCGATAAAGCCCATCACCACCGCCACCCACTGCCCCACTGTCACTCGCTCTTTGAGCAGCGGCGCCGACAGCGCGGTGACCAGCACCGGGGCAAGGAAGTTGACGGCCGTGGCCTCGGCCAGTGGCAGGTATTGCAAGCCGGTCGTGAACAGCAGGCTGGTGCTCAGCAGGCTCAGGGCCCGCAAGGTCTGCAATACCGGCCGGCGGGTGCGCAACACGTTGAGCCCAGACTT  >  KT2440/4165316‑4165575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑