Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,212,263 C→T 7.1% V232I (GTT→ATT)  CJ019_03953 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,212,2630CT 7.1% 176.9 / 2.9 56V232I (GTT→ATT) CJ019_03953hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (20/32);  new base T (2/2);  total (22/34)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

TGATGTTGCGTATCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGCCATGAGTCCTCAACTCTTCGGAAATCAGCCCCCAGGCTCGTCGCCAGCGCGTAGATCTCGCGGAT  >  KT2440/4212131‑4212389
                                                                                                                                    |                                                                                                                              
tgatgTTGCGTATCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCt                                                                                                                              <  6:127817/135‑1 (MQ=255)
tgatgTTGCGTATCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCt                                                                                                                              <  1:235149/135‑1 (MQ=255)
tgatgTTGCGTATCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCt                                                                                                                              <  1:258760/135‑1 (MQ=255)
tgatgTTGCGTATCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCt                                                                                                                              <  3:231179/135‑1 (MQ=255)
 gatgTTGCGTATCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGCACCTcc                                                                                                                            >  7:231043/1‑136 (MQ=255)
 gatgTTGCGTATCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTc                                                                                                                             >  4:200/1‑135 (MQ=255)
     ttGCGTATCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTccccc                                                                                                                         >  1:200979/1‑134 (MQ=255)
         gTATCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCg                                                                                                                     >  4:107105/1‑135 (MQ=255)
         gTATCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGGTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGt                                                                                                                    <  8:30360/136‑1 (MQ=255)
          tATCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGt                                                                                                                    >  1:6939/1‑135 (MQ=255)
          tATCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGt                                                                                                                    <  3:200/135‑1 (MQ=255)
           aTCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCTGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTg                                                                                                                   >  5:214166/1‑135 (MQ=255)
           aTCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGt                                                                                                                    >  3:149252/1‑134 (MQ=255)
            tCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGc                                                                                                                  >  1:146138/1‑135 (MQ=255)
             ccGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTAGTGCCGTTGTCAGCAGCGCTTCCTGACCGTCAAGAAGGTTATCCAGAACGTCCCCTCTCGTGaac                                                                                                                >  7:242453/1‑136 (MQ=255)
               gcgTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGGTATCCAGAACCTCCCCTCTCGTGcact                                                                                                               <  3:68446/135‑1 (MQ=255)
                cgTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGcactg                                                                                                              >  4:178945/1‑135 (MQ=255)
                cgTTCTCATAAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAATCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAACAAGGTTATCCAGACCCTCCGCTCTCGTCcactc                                                                                                              >  2:221332/1‑134 (MQ=255)
                           gATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATaa                                                                                                   <  7:8919/135‑1 (MQ=255)
                                    cTACAGGCGCCCGCAGTTGAATCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAATCTCCCCTCTCg                                                                                                                     >  8:270461/1‑108 (MQ=255)
                                    cTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAATCTCCCCTCTCg                                                                                                                     >  4:77313/1‑108 (MQ=255)
                                    cTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAATCTCCCCTCTCg                                                                                                                     <  7:270461/108‑1 (MQ=255)
                                    cTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAATCTCCCCTCTCg                                                                                                                     <  3:77313/108‑1 (MQ=255)
                                       cAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAg                                                                                      <  8:192145/136‑1 (MQ=255)
                                        aGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGaa                                                                                       >  5:246694/1‑134 (MQ=255)
                                           cgcCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAg                                                                                   <  2:104519/135‑1 (MQ=255)
                                             cccGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGc                                                                                 <  5:218761/135‑1 (MQ=255)
                                             cccGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGc                                                                                 <  6:69011/135‑1 (MQ=255)
                                             cccGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGc                                                                                 <  3:178945/135‑1 (MQ=255)
                                             cccGCAGTAGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTAGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGc                                                                                 <  2:107749/135‑1 (MQ=255)
                                               cGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGcactgatcac                                                                                                         >  7:46920/1‑109 (MQ=255)
                                               cGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGcactgatcac                                                                                                         <  8:46920/109‑1 (MQ=255)
                                               cGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCgg                                                                               >  1:197551/1‑135 (MQ=255)
                                                  aGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAg                                                                           <  8:72707/136‑1 (MQ=255)
                                                  aGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCC‑CTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAg                                                                           <  2:200979/135‑1 (MQ=255)
                                                   gTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATGCTGACCGGCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAg                                                                           <  1:221332/135‑1 (MQ=255)
                                                   gTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATACTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCAGAAGCAGGCGGTCAg                                                                           <  6:86700/135‑1 (MQ=255)
                                                          ctggaagctgTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGc                                                                   <  7:222218/136‑1 (MQ=255)
                                                           tggaagctgTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGc                                                                   <  3:138787/135‑1 (MQ=255)
                                                           tggaagctgTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGc                                                                   <  3:65082/135‑1 (MQ=255)
                                                           tggaagctgTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGc                                                                   <  3:62821/135‑1 (MQ=255)
                                                              aagctgTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGCCa                                                                 <  2:554/134‑1 (MQ=255)
                                                                  tgTCAAAGACCTTCCCTTCTTGCGTTGTCAGCAGCGCATCCTGACCGTAAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGCCATGAGt                                                            <  1:223538/135‑1 (MQ=255)
                                                                     cAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGCCATGAGTCCt                                                         <  6:208160/135‑1 (MQ=255)
                                                                                ccttcTGGCGTTGTCAGCAGCGCATCCTGACCGTCTAGAAGGTTATCGAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGAATGCCGAAGCAGGCGGTCAGCATTACGACATGAGTCCTCAACTCTTCGGa                                             <  8:242453/136‑1 (MQ=255)
                                                                                 cttcTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGACGAAGCAGGCGGTCAGCATTACGCCATGAGTCATCAACTCTTCgta                                             >  7:120074/1‑133 (MQ=255)
                                                                                  ttcTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGCCATGAGTCCTCAACTCTTCGGaa                                            <  2:25633/135‑1 (MQ=255)
                                                                                               agcagcGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGCCATGAGTCCTCAACTCTTCGGAAATCAGCCCCCAgg                               >  4:96078/1‑135 (MQ=255)
                                                                                                    cgcATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGCCATGAGTCCTCAACTCTTCGGAAATCAGCCCCCAGGCtcgt                          <  4:216809/135‑1 (MQ=255)
                                                                                                       aTCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGCCATGAGTCCTCAACTCTTCGGAATTCAGCCCCCAGGCTCGTCGc                       >  5:86973/1‑135 (MQ=255)
                                                                                                          cTGACCGTCAAGAAGGTTATCCAGAACCACCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCCGTCAGCATTACGCCATGAGTCCTCAACTCTTCGGAAATCAGCCCCCAGGCGCGTCGACAg                    >  1:121529/1‑135 (MQ=255)
                                                                                                           tGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGCCATGAGTCCTCAACTCTTCGGAAATCAGCCCCCAGGCTCGTCGCCAgc                   <  5:20175/135‑1 (MQ=255)
                                                                                                                    agaagGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGCCATGAGTCCTCAACTCTTCGGAAATCAGCCCCCAGGCTCGTCGCCAGCGCGTAGAtc          >  3:113443/1‑135 (MQ=255)
                                                                                                                       agGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGCCATGAGTCCTCAACTCTTCGGAAATCAGCCCCCAGGCTCGTCGCCAGCGCGTAGATCTcg       <  6:46932/135‑1 (MQ=255)
                                                                                                                       agGGTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGCCATGAGTCCTCAACGCTTCGGAAATCAGCCCCCAGGCTCGTCGCCAGCGCGTAGATCTcg       <  2:146138/135‑1 (MQ=255)
                                                                                                                            aTCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGCCATGAGTCCTCAACTCTTCGGAAATCAGCCCCCAGGCTCGTCGCCAGCGCGTAGATCTCGCGGAt  >  5:133298/1‑135 (MQ=255)
                                                                                                                                    |                                                                                                                              
TGATGTTGCGTATCCGCGTTCTCATTAGATCAAGTTCTACAGGCGCCCGCAGTTGAAGCTGGAAGCTGTCAAAGACCTTCCCTTCTGGCGTTGTCAGCAGCGCATCCTGACCGTCAAGAAGGTTATCCAGAACCTCCCCTCTCGTGCACTGATCACTGATAATCGATTGCCGAAGCAGGCGGTCAGCATTACGCCATGAGTCCTCAACTCTTCGGAAATCAGCCCCCAGGCTCGTCGCCAGCGCGTAGATCTCGCGGAT  >  KT2440/4212131‑4212389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑