Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,245,145 A→C 47.6% L178R (CTG→CGG)  CJ019_03982 ← Crotonyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,245,1450AC47.6% ‑0.7 / 17.6 35L178R (CTG→CGG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (4/9);  new base C (11/5);  total (15/20)
Fisher's exact test for biased strand distribution p-value = 6.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

GCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCG  >  KT2440/4245015‑4245270
                                                                                                                                  |                                                                                                                             
gcCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGgc                                                                                                                           <  1:21334/135‑1 (MQ=255)
 ccGTCGCGTATCCGCCGCTTGGGCGGGGCATAGGCGAAGGTCGGGCCCTTGGCCCGGCGCCCGGCCCGTTCGGCGGCATCGGCAGGCCACTGCGCGTCGGCGCCGACGTCGCTGGCCCACCCGGCGGCCCggggg                                                                                                                          >  6:29443/1‑133 (MQ=255)
  cGTCGCGTAGCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCGCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGgcgc                                                                                                                         <  4:47831/135‑1 (MQ=255)
                     ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCGATCCGCGCCCCCCAAcac                                                                                                      >  3:189570/1‑133 (MQ=255)
                        cTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCGCGGCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACtcgtcg                                                                                                  <  7:142083/136‑1 (MQ=255)
                              aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCACCCCGGCGGCCCGGGCGCCGTCGGCGCCCCCCACCTCGGCGCGGAAc                                                                                            >  7:252939/1‑136 (MQ=255)
                               tAGGCGACGGGCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAAGGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCGGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAAca                                                                                           <  7:198767/136‑1 (MQ=255)
                                cgccGAAGGGCGGGCCTTGGGCCAGGCGCCCGGCCTGGGCGGCGGAAAGGGCTGGCAACTGCGCGGCGGCGAAGACTTCGCTGACAACCCCGGGGGCCTGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                           <  1:231687/132‑1 (MQ=255)
                                    gAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCTGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAgg                                                                                      <  8:172000/136‑1 (MQ=255)
                                      aGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGCGCGCGCTCCGCGCCCCCCAACTCGTGGAGGTACCGCAGgcg                                                                                    >  7:212808/1‑136 (MQ=255)
                                        gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACCGCAGgcgc                                                                                   >  6:66053/1‑135 (MQ=255)
                                        gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCCCCGTCGGCGCCCCCCAACGCGGCGAGGCACACCAGgccg                                                                                   >  3:43159/1‑133 (MQ=255)
                                               cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGGTCGGCGCCCCCCCACGAGGCGGGGGACAGCCGGCGCGTGGccgg                                                                           >  7:209824/1‑134 (MQ=255)
                                               cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCCCCCACCGCCGCCCACCACTCCGCGAGGCACCGCAGCCGCGTGGccag                                                                           >  7:155514/1‑134 (MQ=255)
                                               cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACAGCTCGTCGGCGCAGCCTTCGCTGACCCACCCCGCGGCCAGCGCGCCCACGGCCCCCCCCCAAGCGGCGACGCACCGCCGGCGCGTGGcca                                                                            >  3:49369/1‑134 (MQ=255)
                                                     ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCGCTCGGCGCCCCCCCACACCGCGCGGCACACCACGCGCGTGGGCTgcgcgc                                                                      >  4:261102/1‑135 (MQ=255)
                                                     ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCACGGCGCGATCGGCGCCCCCCAACTCGGCCAGGAACCCCACGCGCGTGGGCCGCTCGc                                                                      >  4:16213/1‑135 (MQ=255)
                                                        ggCGCCCGGCCTGTGCGGCGGCAACGGCAGGCAACTGCGCGGCGGCGCAGACTGCGCTGCCCACCCCGGCGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                                  <  7:245089/136‑1 (MQ=255)
                                                          cgcCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGGGCCCCCCACCTCGTCGAGGGACAGCCGGCGCTTGGCCAGCTCGCTGCCgc                                                                >  8:130771/1‑135 (MQ=255)
                                                                   tggggggcggcAACGGAAGGCACCTGATCGCCGGCCCTCCATGCGCAGACCACCCGGGGGGCGTGGGCGGGTGCGCCGCCCCACAACTCGTCGAGGAACAGCTGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                        <  2:273265/130‑1 (MQ=255)
                                                                   aGTGCGGGGGAAAGGGCTGGCAACTGCTCGGCGGGGCTGACTTCGCTGACCACCCCGGCGGCCTGGGCGCGTGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                        <  6:132662/135‑1 (MQ=255)
                                                                   aGTGCGGCGGCACGGGCAGGCAACTGGGCGGCGGCGCAGACTTCGCGCACCAACCCGGCGGCCCGGGGGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                        <  1:190865/135‑1 (MQ=255)
                                                                             cAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCAACGCGTCGCGGCACCGCAGGCGCGTGGCCGGCTCGCGGGCGCTCCCCCGGGGCCGGTGccc                                             >  7:63128/1‑135 (MQ=255)
                                                                                  gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATAGCCGCCCCACCACACCTCCAGGCACAGCCGGCGCTTGGCCTGCTCCCAGCCGCACACCCCGGGCAGGTGACAGCTg                                         >  6:218246/1‑135 (MQ=255)
                                                                                                gtcgGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGCTCGGCGCCCCCCAACTCGTCGAGGAACAGCCGGCGCGTGGCCTGCTCGCGGCCGCTCAGCCGGGGCCGGTGCCCGCGGGCGCCGGCCTCggg                           >  5:135373/1‑135 (MQ=255)
                                                                                                    gcgcTGACTGCGCTGACCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                      <  8:22014/136‑1 (MQ=255)
                                                                                                     cgcAGACTTCGCTGACCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                     <  8:194905/136‑1 (MQ=255)
                                                                                                     cgcAGACTTCGCGGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                      <  4:189570/135‑1 (MQ=255)
                                                                                                      gcAGACTCCGCTGACCAACCGGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                     <  1:162809/135‑1 (MQ=255)
                                                                                                          aCTTCGCGCCCCAACCCGGCGCCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc                 <  5:136469/135‑1 (MQ=255)
                                                                                                                   ccccaCCGGGGGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt       <  7:79703/132‑1 (MQ=255)
                                                                                                                   aCCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt       <  8:209824/136‑1 (MQ=255)
                                                                                                                    ccACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt       <  4:262796/135‑1 (MQ=255)
                                                                                                                    ccAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt       <  4:218332/135‑1 (MQ=255)
                                                                                                                         cgggcggcCGGGGGGCGCGCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCg  <  5:218800/133‑1 (MQ=255)
                                                                                                                                  |                                                                                                                             
GCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCG  >  KT2440/4245015‑4245270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑