Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,287,611	T→G	26.3%	D13A (GAC→GCC)	CJ019_04019 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,287,611	0	T	G	26.3%	68.0 / 5.5	44	D13A (GAC→GCC)	CJ019_04019	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (25/6);  new base G (8/4);  total (33/11)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGGGCGGTAGCGCTGCAGAATGGCCTTGCGCAGGCGCACCAGTTCATCTTTGTCGTCGCCCTTGTCGAGCACGAAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGTATTTGGCAAAGCAGCTGCTTAGAAGATCGAGAAGTTGTAGCTGA  >  KT2440/4287479‑4287741                                                                                                                                     |                                                                                                                                   tCGGGGCGGTAGCGCTGCAGAATGGCCTTGCGCAGGCGCACCAGTTCATCTTTGTCGTCGCCCTTGTCGAGCACGAAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGCTGACCTGGAGCGGGTCAc                                                                                                                                 >  8:230466/1‑136 (MQ=255)      gCGGTAGCGCTGCAGAATGGCCTTGCGCAGGCGCACCAGTTCATCTTTGTCGTCGCCCTTGTCGAGCACGAAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGGCACCcgcg                                                                                                                            >  7:241470/1‑136 (MQ=255)      gCGGTAGCGCTGCAGAATGGCCTTGCGCAGGCGCACCAGTTCATCTTTGTCGTCGCCCTTGTCGAGCACGAAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGGCACCcgcg                                                                                                                            >  7:246879/1‑136 (MQ=255)            gcgcTGCAGAATGGCCTTGCGCAGGCGCACCAGTTCATCTTTGTCGTCGCCCTTGTCGAGCACGCAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTGCATAGGTGACCTGGCGCGGGGCCCCCGCGCCgct                                                                                                                       >  1:38595/1‑133 (MQ=255)            gcgcTGCAGAATGGCCTTGCGCAGGCGCACCAGTTAATCTTTGTCGTCGCCCTTGTCGAGCACGAAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGTCccccgccccgca                                                                                                                       >  1:50988/1‑133 (MQ=255)                          ccTGGCGCCGGCGCACCTGTTCATATTTGTCGGCGCCGTTGGCGAGCACTACGAACACGCTGGCCCTGCGCAGCGCATCGACCGGTTCATCGGTGACCTGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAg                                                                                                        <  7:21072/136‑1 (MQ=255)                           cTTGCGCAGCCGAACCAGTTCATCTTTGTCGCCGCCCTTGTCGACCACGAACAACAGGCTGGCACTGCGCTGCGCTTCGACCGCTTATTAGGTCACGTGGGGGGGGTCACCCGCGCCGATACCGATCAACAACAg                                                                                                        <  2:19077/135‑1 (MQ=255)                             tGCGCAGGCGCACCAGTTCATCTTTGTCGTCGCCCTTGTCGAGCACGAAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGGCCCCCGCGCCGATGACGATTCAAAACAGGt                                                                                                      >  2:249858/1‑135 (MQ=255)                                  aGGCGCACCAGTTCATCTTTGTCGTCGCCCTTGTCGAGCACGAAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGTCCCCCGCGCCGGTACCGCTCAACCACAGGtggtt                                                                                                  >  3:164699/1‑130 (MQ=255)                                       cACCAGTTCATCTTTGTCGTCGCCCTTGTCGAGCACGAAGACCACGCTGGCACTGCGCAGCGCATCGCCCGCTTCATAGGTGACCTGGCGCGGGGCCCCCGCCGCGCTCCCGCTCCGCAACAGCTCTTTCCTtga                                                                                            >  4:22663/1‑134 (MQ=255)                                       cACCAGTTCATCTTTGTCGTCGCCCTTGTCGAGCACGAAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGCCCTGGCGCGGGGCCCCCGCGCCGCTCCCGCTCAACAACAGGTCTTTCAGtgcc                                                                                           >  7:228066/1‑136 (MQ=255)                                       cACCAGTTCATCTTTGTCGTCGCCCTTGTCGAGCACGAAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGTCCCCCGCGCCGCGACCGATCAACAACAGGTCGTTCATtgc                                                                                            >  5:129610/1‑135 (MQ=255)                                                     tGTCGTCGCCCTTGTCGAGCACGAAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGTc                                                                                                                                   >  7:219009/1‑82 (MQ=255)                                                     tGTCGTCGCCCTTGTCGAGCACGAAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGTc                                                                                                                                   <  8:219009/82‑1 (MQ=255)                                                          tcgCCCTTGTCGAGCACGAAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAAcc                                                                         >  3:201769/1‑135 (MQ=255)                                                          tcgCCCTTGTCGAGCACGAAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGGCCCCCCCCCCGCTAACCCTCAACAACACGGCTTTGATTTCCCAGCCCGTTGCGGGGAcc                                                                         >  5:13850/1‑135 (MQ=255)                                                               attGCCGAGCACTACCAACACGCGGGCACTGCGCTGCTCTTCCACCGCTTCATAGTGCACCTGCCGGGGGGCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGa                                                                    <  2:57952/134‑1 (MQ=255)                                                                    cGAGCACGAAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCAtt                                                               >  4:269537/1‑135 (MQ=255)                                                                           gaagaaCACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGc                                                        >  1:3220/1‑135 (MQ=255)                                                                           gaagaaCACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCg                                                       >  8:157991/1‑136 (MQ=255)                                                                              ctccaggCTGGCCCGGCGCAGCGCATCGACCGCTCCTTCTGTCACATGGCGGGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCTGGCGGt                                                     <  4:218768/129‑1 (MQ=255)                                                                                acacGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGTCCCCCGCGCGGATACCGCTCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGCCCATTGTCGGGCGGTTa                                                   >  3:192265/1‑135 (MQ=255)                                                                                        gCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGTCCCCCGCGCCGCTACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGCACCCGCGCCCAGTGTCGGGCGGTTACCGGGAtt                                           >  3:274364/1‑135 (MQ=255)                                                                                         cACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATCGCCCTGCCCTTTGCGGGCACCCGCGCCCATTGTCGGGCGGTTACCGGGATTTg                                         >  8:217319/1‑136 (MQ=255)                                                                                               gcagcgcaTCGACCGCTTCATAGGTGACCTGGCGCGGGTCACCCGCGCCGCTACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCCCGACCATTGTCGGGCGGTTACCGGTATTTGGCaaa                                    >  3:120894/1‑135 (MQ=255)                                                                                                aacgcaaTCCACCGCTTCATAGGGCCCCGGGCGCGGGCCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGTATTTGGCAAag                                   <  4:164699/129‑1 (MQ=255)                                                                                                 agcgcaTCGACCGCTTCATAGGTGACCTGGCGCGGGTCACCCGCGCCGCGACCGAGCACCCACAGGTCTTTCCTTGCCCTGCCCGTTGCGGGAACCCGCGCCCATTGGCGGGCGGTGACCGGTATTTGGCaaa                                    >  1:418/1‑133 (MQ=255)                                                                                                 agcgcaTCGACCGCTTCATAGGTGACCTGGCGCGGGTCACCCGCGCCGATACCGATCAACAACTGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGTATTTGGCaaa                                    >  3:241343/1‑133 (MQ=255)                                                                                                 agcgcaTCGACCGCTTCATAGGTGACCTGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGTATTTGGCaaa                                    >  4:94417/1‑133 (MQ=255)                                                                                                 agcgcaTCGACCGCTTCATAGGTGACCTGGCGCGGGGCACCCGCGCCCGTACCGCTGAAAAACAGGTCTTTAATTTCCCTTCCCGTTTCGGGAACCCCCCCCCCTTTTCGGGCGGGTTGCGGTATTTTGCaaa                                    >  6:179877/1‑133 (MQ=255)                                                                                                      aTCGACCGCTTCATAGGTGACCTGGCGCGGGTCACCCGCGCCGATACCGATCAACCACAGGGCTTTCCTTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGGTACCGGGATTTGGCAAAGCAgctg                             >  4:29555/1‑135 (MQ=255)                                                                                                      aTCGACCGCTTCATAGGTGACCTGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGTATTTGGCAAAGCAgctg                             >  2:37317/1‑135 (MQ=255)                                                                                                           ccGCTTCATAGGTGACCTGGCGCGGGTCACCCGCGCCGCTCCCGAACAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGTATTTGGCAAAGCAGCTGCTTa                         >  4:74689/1‑134 (MQ=255)                                                                                                           ccGCTTCATAGGTGACCTGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTTCGGGAACCCGCGACCATTGGCGGGCGGTTACCGGTATTTGGCAAAGCAGCGGCGTag                        >  7:271376/1‑135 (MQ=255)                                                                                                                tCATAGGTGACCTGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGGATTTGGCAAAGCAGCTGCTTag                        >  8:255813/1‑130 (MQ=255)                                                                                                                  atgggTGCCCGGGGGGGGGGCACCGGGGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGTATTTGGCAAAGCAGCTGCTTAGAAGAt                   <  3:257250/130‑1 (MQ=255)                                                                                                                  aTAGGTGACCTGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCCACCATTGTCGGGCGGTTACCGGTATTTGGCAAAGCAGCTGCTTAGAAGAt                   >  6:273197/1‑133 (MQ=255)                                                                                                                           gtGGCGGGGGTCCCCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGTATTTGGCAAAGCAGCTGCTTAGAAGATCGAGAAGTTGt        <  4:61363/134‑1 (MQ=255)                                                                                                                            tGGCGCGGGTCACCCGCGCCGATACCGATCAACAACTGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGTATTTGGCAAAGCAGCTGCTTAGTAGATCGAGAAGTTGTa       <  4:241343/135‑1 (MQ=255)                                                                                                                            tGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGTATTTGGCAAAGCAGCTGCTTAGAAGATCGAGAAGTTGTa       >  4:116003/1‑135 (MQ=255)                                                                                                                            tGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGTATTTGGCAAAGCAGCTGCTTAGAAGATCGAGAAGTTGTAg      >  7:43560/1‑136 (MQ=255)                                                                                                                            tGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGGATTTGGCAAAGCAGCTGCTTAGAAGATCGAGAAGTTGTa       >  4:246882/1‑135 (MQ=255)                                                                                                                                gcgGGGCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGTATTTGGCAAAGCAGCTGCTTAGAAGATCGAGAAGTTGTAGCTg   <  4:214111/135‑1 (MQ=255)                                                                                                                                 cgGGGCCCCCGCGCCGATAACGATCAACAAGAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGTATTTGGCAAAGCAGCTGCTTAGAAGATCGAGAAGTTGTAGCTGa  <  4:166718/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   TCGGGGCGGTAGCGCTGCAGAATGGCCTTGCGCAGGCGCACCAGTTCATCTTTGTCGTCGCCCTTGTCGAGCACGAAGAACACGCTGGCACTGCGCAGCGCATCGACCGCTTCATAGGTGACCTGGCGCGGGTCACCCGCGCCGATACCGATCAACAACAGGTCTTTCATTGCCCTGCCCTTTGCGGGAACCCGCGACCATTGTCGGGCGGTTACCGGTATTTGGCAAAGCAGCTGCTTAGAAGATCGAGAAGTTGTAGCTGA  >  KT2440/4287479‑4287741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑