Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,293,337	A→T	6.4%	Q108L (CAG→CTG)	CJ019_04028 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,293,337	0	A	T	6.4%	229.8 / 4.1	72	Q108L (CAG→CTG)	CJ019_04028	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (27/39);  new base T (2/3);  total (29/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

CGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCCGGCCTGGTCGGTGACGAAGTGT  >  KT2440/4293206‑4293471                                                                                                                                    |                                                                                                                                       cGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCTCACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGAt                                                                                                                                     >  2:210440/1‑135 (MQ=255)  gTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATg                                                                                                                                    >  1:106284/1‑135 (MQ=255)      cccGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTgg                                                                                                                                >  4:122311/1‑135 (MQ=255)       ccgccgCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAAGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGcc                                                                                                                              <  7:160746/136‑1 (MQ=255)       ccgccgCATCGAGGGCATCGAAGGCTGGCCACGGGGCTGGTTCGACGCGCTGGCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGcc                                                                                                                              <  7:53057/136‑1 (MQ=255)       ccgccgCATCGAGGGCAGCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGcc                                                                                                                              <  7:240822/136‑1 (MQ=255)             cATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAg                                                                                                                         >  4:61529/1‑135 (MQ=255)             cATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGc                                                                                                                        >  8:77648/1‑136 (MQ=255)              ctCGAGGGCATCGAAGGCTGGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCTAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGACGc                                                                                                                        <  3:141650/134‑1 (MQ=255)                  aGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGc                                                                                                                     <  2:200774/134‑1 (MQ=255)                     gCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAgg                                                                                                                >  7:140580/1‑136 (MQ=255)                       aTCGAAGGCTTGCCACGGGGCTGGTTCGCCGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACACGCTCTCTGCAGCCGCCCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGt                                                                                                               >  4:57631/1‑135 (MQ=255)                        tCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTa                                                                                                              >  5:40422/1‑135 (MQ=255)                        tCGAAGGCTTGCCACGGGGCTGGTGCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTa                                                                                                              <  1:183919/135‑1 (MQ=255)                           aaGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTAtctc                                                                                                          >  8:68298/1‑136 (MQ=255)                                    cACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGaaa                                                                                                  >  1:233682/1‑135 (MQ=255)                                     aCGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAAc                                                                                                 >  5:146149/1‑135 (MQ=255)                                        gggCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCgg                                                                                             <  7:64631/136‑1 (MQ=255)                                         ggCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCgg                                                                                             <  5:123611/135‑1 (MQ=255)                                         ggCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCGCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCgg                                                                                             <  3:63430/135‑1 (MQ=255)                                           cTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACTGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGc                                                                                            >  5:208116/1‑134 (MQ=255)                                           cTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACTGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGc                                                                                            >  5:204641/1‑134 (MQ=255)                                           cTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGc                                                                                            >  5:266207/1‑134 (MQ=255)                                               ttCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGgc                                                                                      <  8:50916/136‑1 (MQ=255)                                                      cgcTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCGAAGGCTGCTgg                                                                                <  4:8920/135‑1 (MQ=255)                                                      cgcTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTgg                                                                                <  3:43680/135‑1 (MQ=255)                                                      cgcTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTgg                                                                                <  5:46426/135‑1 (MQ=255)                                                      cgcTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTgg                                                                                <  6:190006/135‑1 (MQ=255)                                                      cgcTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTgg                                                                                <  1:62524/135‑1 (MQ=255)                                                      cgcTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTgg                                                                                >  1:69011/1‑135 (MQ=255)                                                             gCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAgcggcg                                                                        <  8:18166/136‑1 (MQ=255)                                                             gCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAgcggc                                                                         <  8:212295/135‑1 (MQ=255)                                                              ccAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACCAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAgcggc                                                                         <  1:19670/134‑1 (MQ=255)                                                              ccAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAgcggcg                                                                        <  2:233682/135‑1 (MQ=255)                                                              ccAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAgcggc                                                                         <  3:122311/134‑1 (MQ=255)                                                                  gctaaCACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAag                                                                    <  6:234262/132‑1 (MQ=255)                                                                     aaCACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGc                                                                 >  2:178024/1‑135 (MQ=255)                                                                      acacGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCt                                                               <  7:228918/136‑1 (MQ=255)                                                                        acGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCt                                                               >  4:95631/1‑134 (MQ=255)                                                                         cGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGc                                                             <  6:58612/135‑1 (MQ=255)                                                                             caAGCGGCTGTCGAGCCAGGGGACAACAAGCGCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATg                                                        <  8:21159/136‑1 (MQ=255)                                                                              aaGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATg                                                        <  5:276334/135‑1 (MQ=255)                                                                              aaGCGGCTGGCGAGCCAGGTGACAACAAGCGCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATg                                                        <  3:120098/135‑1 (MQ=255)                                                                               aGCGGCTGTAGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCGGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAAACCGGCACAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGcc                                                      <  7:197357/136‑1 (MQ=255)                                                                                     tggcGAGCCAGGTGACAACAAGCGCTCTGCAGCCGACCTGGTCAAACCGATGCTCGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACgct                                                 <  2:184660/132‑1 (MQ=255)                                                                                        cGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACgctg                                                <  6:211397/133‑1 (MQ=255)                                                                                         gAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAGGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACgctgct                                              >  2:172984/1‑134 (MQ=255)                                                                                         gAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTgg                                                                                <  8:154478/100‑1 (MQ=255)                                                                                         gAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTgg                                                                                >  7:154478/1‑100 (MQ=255)                                                                                              aGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGCTGCTGGCCCGAAGCGGCAAAGGTCTCTCCGAAGAAACCCGGCAAAGCCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGcc                                       <  8:85590/136‑1 (MQ=255)                                                                                               ggTGACAACAAGCTCTCTGCAGCCGACCTGGGCAACCAGATGCGGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAACGCTGCTGGCAGCGGCGCAAGAGCCTGCAGGTGCCACGCTGCTGGAGGcc                                       <  6:225129/135‑1 (MQ=255)                                                                                                 tGACAACAAGCTCTCTGCAGCCGACCTGGCCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCAGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAg                                    >  7:8441/1‑136 (MQ=255)                                                                                                   acaacaAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGcc                                  <  8:206915/136‑1 (MQ=255)                                                                                                    caacaaGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGcc                                  >  4:96695/1‑135 (MQ=255)                                                                                                        aaGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTc                             <  8:174301/136‑1 (MQ=255)                                                                                                         aGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTc                             <  1:15947/135‑1 (MQ=255)                                                                                                             ctctGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGcccc                        >  8:146133/1‑136 (MQ=255)                                                                                                                 gCAGCCGCCCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCCGGcc                    >  7:266148/1‑136 (MQ=255)                                                                                                                 gCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGcccc                        <  3:56385/132‑1 (MQ=255)                                                                                                                 gCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCCg                       <  1:52029/133‑1 (MQ=255)                                                                                                                 gCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCCGGc                     >  6:75159/1‑135 (MQ=255)                                                                                                                  cAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCTGGcc                    >  5:112999/1‑135 (MQ=255)                                                                                                                   aGCCGACCTGGTCAAACTGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCCGGCCt                   <  6:204641/135‑1 (MQ=255)                                                                                                                   aGCCGACCTGGTCAAACTGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCCGGCCt                   <  6:208116/135‑1 (MQ=255)                                                                                                                   aGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCCGGCCt                   <  3:227411/135‑1 (MQ=255)                                                                                                                     ccGAACTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTCTCTCCGAAGACACCCGGCTACGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCCGGCCTgg                 <  6:227703/135‑1 (MQ=255)                                                                                                                        aCCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCCGGCCTGGTCg              >  6:252665/1‑135 (MQ=255)                                                                                                                         ccTGGTCAAACTGATGCTGGCCGGAAGCTGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGAGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCCGGCCTGGTCGGt            <  8:245405/136‑1 (MQ=255)                                                                                                                           tGGTAAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCCGGCCTGGGCgggg           >  1:242322/1‑133 (MQ=255)                                                                                                                               cAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGACTGCAGATGCCACGCTGCTGGAGGCCGCGCCGCTTAGCCCCGGCCAGGTAGGTGACGa       >  1:117836/1‑135 (MQ=255)                                                                                                                                aaaCAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCCGGCCTGGTCGGGGACGaa      >  2:139252/1‑135 (MQ=255)                                                                                                                                   cAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCCGGCCTGGTCGGTGACGAAgtgt  <  8:140580/136‑1 (MQ=255)                                                                                                                                    aGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCCGGCCTGGTCGGTGACGAAgtgt  <  5:259161/135‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       CGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGCGGCAAAGGTATCTCCGAAGAAACCCGGCAAAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCCGGCCTGGTCGGTGACGAAGTGT  >  KT2440/4293206‑4293471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑