Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,400,445	G→A	9.7%	I348I (ATC→ATT)	CJ019_04157 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,400,445	0	G	A	9.7%	120.7 / 4.0	41	I348I (ATC→ATT)	CJ019_04157	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (17/20);  new base A (2/2);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

TCCGATCAAGCCAGGCGTGCCACTTCTCCAGCGCCAACTTGCGCTGCTCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGCCCCAGCCGGGTGAACACCATGCTCGCCTGGGTCTCACTCAGGCAGTGGCCGCGACGGTTCG  >  KT2440/4400318‑4400572                                                                                                                                |                                                                                                                                tccgatcAAGCCAGGCGTGCCACTTCTCCAGCGCCAACTTGCGCTGCTCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGa                                                                                                                          <  1:153523/135‑1 (MQ=255) tccgatcAAGCCAGGCGTGCCACTTCTCCAGCGCCAACTTGCGCTGCTCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGa                                                                                                                          <  3:252283/135‑1 (MQ=255)      tcAAGCCAGGCGTGCCACTTCTCCAGCGCCAACTTGCGCTGCTCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGgtccg                                                                                                                    <  8:209685/136‑1 (MQ=255)          gCCAGGCGTGCCACTTCTCCAGCGCCAACTTGCGCTGCTCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGgtccgtcc                                                                                                                 <  6:60973/135‑1 (MQ=255)                  tGCCACTTCTCCAGCGCCAACTTGCGCTGCTCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCAAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGTTGCCGAGGTCCGTCCAGGtgctgc                                                                                                        >  8:132680/1‑136 (MQ=255)                       cTTCTCCAGCGCCAACTTGCGCTGCTCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGa                                                                                                    >  2:12847/1‑135 (MQ=255)                       cTTCTCCAGCGCCAACTTGCGCTGCTCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCAATGCCGAGGTCCGTCCAGGTGCTGCGGGa                                                                                                    >  1:162710/1‑135 (MQ=255)                       cTTCTCCAGCGCCAACTTGCGCTGCTCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCAATGCCGAGGTCCGTCCAGGTGCTGCGGGa                                                                                                    >  1:167015/1‑135 (MQ=255)                       cTTCTCCAGCGCCAACTTGCGCTGCTCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGGGTGGTTCCGCAGCATCTCGCCGATGTGGCCGGCGATGCCGATGTCCGTCCAGGTGCGGCgggcc                                                                                                   >  8:194802/1‑134 (MQ=255)                              aGCGCCAACTTGCGCTGCTCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCt                                                                                               <  3:258567/133‑1 (MQ=255)                                cgcCAACTTGCGCTGCTCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTgcg                                                                                           >  5:147129/1‑135 (MQ=255)                                  ccAAATTGCGCTGCTCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCa                                                                                         <  4:52783/135‑1 (MQ=255)                                     aCTTGCGCTGCTCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGt                                                                                      >  4:185846/1‑135 (MQ=255)                                           gctgctCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGc                                                                                <  5:64608/135‑1 (MQ=255)                                                  cATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGAt                                                                                                                               >  3:38067/1‑81 (MQ=255)                                                  cATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGAt                                                                                                                               <  4:38067/81‑1 (MQ=255)                                                  cATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCa                                                                         >  2:61064/1‑135 (MQ=255)                                                     ggCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTc                                                                      <  5:58215/135‑1 (MQ=255)                                                       ccTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCCCTCGc                                                                    >  6:146155/1‑135 (MQ=255)                                                       ccTGGGTGTGGAGGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTc                                                                      <  1:33618/133‑1 (MQ=255)                                                            gtgtGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCATCATCTCGCCGATGAGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGc                                                               >  2:82758/1‑135 (MQ=255)                                                            gtgtGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCtt                                                                                              <  8:264841/104‑1 (MQ=255)                                                            gtgtGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCtt                                                                                              >  7:264841/1‑104 (MQ=255)                                                               tgGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGcccc                                                            <  7:76506/135‑1 (MQ=255)                                                                    gTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCCCTCGCCCTGCCCCAGCCg                                                       >  4:103437/1‑135 (MQ=255)                                                                    gTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGCCCCAGCCg                                                       >  2:68706/1‑135 (MQ=255)                                                                    gTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCAATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGCCCCAGCCg                                                       <  2:167015/135‑1 (MQ=255)                                                                    gTAGGTGCTGGCGATCTTGCCCAGCGTGTGGGTCAGCAGCATCTCGCCGATGTGGCCGTCAATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGCCCCAGCCg                                                       <  2:162710/135‑1 (MQ=255)                                                                          gCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACCCCTTGCGCAGGTCGTGGCGGGGGCCCGCGCCCGGCCCCCGCCgggggcc                                                 >  5:226883/1‑131 (MQ=255)                                                                                gATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGCCCCAGCCGGGTGAACACCAt                                           >  1:13444/1‑135 (MQ=255)                                                                                      gCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGCCCCAGCCGGGTGAACACCATGCTCg                                      <  8:9588/134‑1 (MQ=255)                                                                                      gCCCAGCGGGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGCCCCAGCCGGGTGAACACCATGCTCGcc                                    >  8:86575/1‑136 (MQ=255)                                                                                       cccAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGCCCCAGCCGGGTGAACACCATGCTCg                                      <  6:58493/133‑1 (MQ=255)                                                                                       cccAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGCCCCAGCCGGGTGAACACCATGCTCg                                      <  1:12847/133‑1 (MQ=255)                                                                                        ccAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGCCCCAGCCGGGTGAACACCATGCTCGCCTg                                  <  7:70197/136‑1 (MQ=255)                                                                                         cAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGCCCCAGCCGGGTGAACACCATGCTCGCCTg                                  <  1:79214/135‑1 (MQ=255)                                                                                                             tctcGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGGCGTGGCTGGTCCACTCGCCCTGCCCCAGCCGGGTGAACACCATGCTCGCCTGGGTCTCACTCAGGCAGTGGc              <  4:18836/135‑1 (MQ=255)                                                                                                                cGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTGCCCTCGCCCTGCCCCAGCCGGGTGAACACCATGCTCGCCTGGGTCTCACGCAGGCAGTGGCcgc           >  5:128818/1‑135 (MQ=255)                                                                                                                    gATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGCCCCAGCCGGGTGAACACCATTCTCGCTTGGGTCTCACTCAGTCAGTGGCCGCGACg       <  5:146155/135‑1 (MQ=255)                                                                                                                        tgGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGCCCCAGCCGGGTGCACACCATGCTCGCCTGGGTCTCACTCAGGCAGTGGCCGCGACGGTTc   >  6:179023/1‑135 (MQ=255)                                                                                                                         ggCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGCCCCAGCCGGGTGAACACCATGCTCGCCTGGGTCTCACTCAGGCAGTGGCCGCGACGGTTCg  <  3:103437/135‑1 (MQ=255)                                                                                                                                |                                                                                                                                TCCGATCAAGCCAGGCGTGCCACTTCTCCAGCGCCAACTTGCGCTGCTCCATGGCCTGGGTGTGGATGTAGGTGCTGGCGATCTTGCCCAGCGTGTGGTTCAGCAGCATCTCGCCGATGTGGCCGTCGATGCCGAGGTCCGTCCAGGTGCTGCGGGACACCTTGCGCAGGTCGTGGCTGGTCCACTCGCCCTGCCCCAGCCGGGTGAACACCATGCTCGCCTGGGTCTCACTCAGGCAGTGGCCGCGACGGTTCG  >  KT2440/4400318‑4400572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑