Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,749,286 T→G 32.7% S294A (TCG→GCG)  macB → Macrolide export ATP‑binding/permease proteinMacB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,749,2860TG32.7% 30.1 / 6.8 40S294A (TCG→GCG) macBMacrolide export ATP‑binding/permease proteinMacB
Reads supporting (aligned to +/- strand):  ref base T (11/15);  new base G (8/6);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGGCG  >  KT2440/4749155‑4749413
                                                                                                                                   |                                                                                                                               
aCCGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTc                                                                                                                                <  4:80659/133‑1 (MQ=255)
       tgCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCggtggtggg                                                                                                                       >  2:26540/1‑134 (MQ=255)
       tgCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGggggtggtg                                                                                                                      >  7:242100/1‑136 (MQ=255)
                         cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGt                                                                                                       >  4:71324/1‑133 (MQ=255)
                         cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGGGGTGGTGATGTTGGGTGTGGGt                                                                                                       >  1:91349/1‑133 (MQ=255)
                         cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCCGTGGTGGTGATGTTTGCTGTGGGTGa                                                                                                     >  3:49172/1‑135 (MQ=255)
                         cTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAATGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGGGGTGATGTTTGCGGGCGGt                                                                                                       >  3:36702/1‑133 (MQ=255)
                                   gAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGGGGTGATGTTGGCTGTCGGTGAAGGCAGCaaa                                                                                           <  1:24004/135‑1 (MQ=255)
                                    aaCTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACg                                                                                         >  7:72289/1‑136 (MQ=255)
                                          tCGAATCGCGGCAGGCGGCTTGGCGAGTGATGTGGAGCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGGCGGTGAAGGCAGCAAACGCCa                                                                                      <  3:202786/133‑1 (MQ=255)
                                            gAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGGGGTGGTGATGTTGGCTGTCGGTGAAGGCCGCAAACGCCAGGTa                                                                                  >  5:97928/1‑135 (MQ=255)
                                                 gCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCCCTGACCCTGCTCGGAATCATCATCGGAGTCGCGGGGGTGGGGGTGATGTTGTCTGTCGGTGTAGGCAGCCAACGCCAGGGAATGGc                                                                             >  5:175847/1‑135 (MQ=255)
                                                           gcttGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATGGGAGTCGCGGCGGTGGTGGTGAGGTGGGCTGTCGGGGAAGGCAGCAAACGCCAGGTAATGGCACAGATGgcc                                                                   <  2:80039/132‑1 (MQ=255)
                                                               ggCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGGGGGGGGGGTGTTGTTGGGGGGGGGTGGAGGCCGCACACGCCAAGTTATGGCACAGATGGCCGCCt                                                               >  4:143776/1‑135 (MQ=255)
                                                                gCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGgccgccgt                                                              >  3:111527/1‑133 (MQ=255)
                                                                gCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTc                                                             >  8:50647/1‑136 (MQ=255)
                                                                gCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGGGGGGGGGGTGGTGTTGGCTGGGGGTGGAGGCCGGCGCCGCCCGGGAATGGCCCCGATGgccgccgg                                                              >  5:26295/1‑133 (MQ=255)
                                                                   agggaggtgGATCAACCGCTGCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCACCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCgg                                                           <  5:242296/129‑1 (MQ=255)
                                                                      gATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGGCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTc                                                        <  5:239837/135‑1 (MQ=255)
                                                                      gATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGCAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCt                                                         <  1:26540/134‑1 (MQ=255)
                                                                      gATGGGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGAAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTc                                                        <  3:71324/135‑1 (MQ=255)
                                                                        tgtgGATCAACCGCTTCCGCACGGCACTGAACCTGCTCGGAATCATCATCGGAGTCGCGTCCGGGGTGGGTTTGTTGGGTGTGCGGGCAGGCCGCAAACCCCCCGGAAAGGCACCGAAGGCCGCCGGAGGCTccca                                                     >  8:209396/1‑134 (MQ=255)
                                                                         gtgGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGTGGGGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCa                                                      <  1:150168/134‑1 (MQ=255)
                                                                            gATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATc                                                 <  8:198955/136‑1 (MQ=255)
                                                                             aTCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGTGGTGGTGATGTTGGCTGGCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATcc                                                <  8:72289/136‑1 (MQ=255)
                                                                             aTCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGTGGTGGTGATGTTGGCTGGCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATc                                                 <  3:216342/135‑1 (MQ=255)
                                                                                aaCCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGGCGGTGGTGGTGTTGTTGGGTGTGGGTGTAGGCAGCAAACGCCAGGTAATTGCACAGATTGCCGCCTTCGGGGCCAACaaact                                               >  4:216342/1‑130 (MQ=255)
                                                                                         cGCCCGGCACTGACCCTGCTCGGAATCATCATCGGAGGCGCGTCGGGGGGGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAAt                                     <  2:129112/135‑1 (MQ=255)
                                                                                          gCACGCCACTGCCCCTGCGCGGAATCATCATCGGAGTCGCGTCGGTGGTTGGTATGTGGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATg                                    <  6:175847/135‑1 (MQ=255)
                                                                                           cACGGCACTGACCCTGCTCGGAATCATCATCGGAGGCGCGTCGGTGGTGGTGATGTTGGCTGTCGGGGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATgg                                   <  1:243106/135‑1 (MQ=255)
                                                                                                            tCGGAATCATCATCGGAGGCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGCCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCt                    <  2:91349/133‑1 (MQ=255)
                                                                                                             cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGAt                                                                                                                    >  2:205126/1‑36 (MQ=255)
                                                                                                             cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGAt                                                                                                                    <  1:205126/36‑1 (MQ=255)
                                                                                                             cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAAc                                                    >  7:182258/1‑100 (MQ=255)
                                                                                                             cGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAAc                                                    <  8:182258/100‑1 (MQ=255)
                                                                                                             cGGAATCATCATCGGAGTCGCGGCGGTGGTGGTGATGTTGGCTGTCGGTGCAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGGTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTgcgc                >  7:222789/1‑136 (MQ=255)
                                                                                                                  tcatcatcGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCt                                                         >  2:56968/1‑90 (MQ=255)
                                                                                                                  tcatcatcGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCt                                                         <  1:56968/90‑1 (MQ=255)
                                                                                                                      catcGGAGTCGCGGCGGGGGTGGGGATGTTGGCTGGCGGTGAAGGCAGAAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGc        <  4:36702/135‑1 (MQ=255)
                                                                                                                           gAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGgcg  <  7:50647/136‑1 (MQ=255)
                                                                                                                                   |                                                                                                                               
ACCGTGGTGCAACCCAGCACGGTGCCTGGAAAGGTGAACTGCTCGAATCGCTGCAGGCGGCATGGCGAGTGATGTGGATCAACCGCTTCCGCACGGCACTGACCCTGCTCGGAATCATCATCGGAGTCGCGTCGGTGGTGGTGATGTTGGCTGTCGGTGAAGGCAGCAAACGCCAGGTAATGGCACAGATGGCCGCCTTCGGCTCCAACATCCTATACCTCAATGGCAGCCCCCCGACCCTGCGCGAACCGGCTGGGCG  >  KT2440/4749155‑4749413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: