Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 253,344 T→G 19.0% L137V (TTG→GTG)  ssuC_1 → Putative aliphatic sulfonates transport permeaseprotein SsuC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440253,3440TG19.0% 48.0 / 2.4 32L137V (TTG→GTG) ssuC_1Putative aliphatic sulfonates transport permeaseprotein SsuC
Reads supporting (aligned to +/- strand):  ref base T (8/16);  new base G (4/3);  total (12/20)
Fisher's exact test for biased strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCATCCTGCTCAACACCGTGCATGGCGTCGAGGCCGTCGACCCGCGCTTGGTGGCATCGGCGCGCAGCCTCGGTGCCGGGCGCTGGGCGATCCTGCGCGAGGTGGTATTGCCTGGAGCGTTGCCGAG  >  KT2440/253211‑253477
                                                                                                                                     |                                                                                                                                     
aTGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCgtt                                                                                                                                      <  3:214817/135‑1 (MQ=255)
  gggCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGGCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCgttgt                                                                                                                                    <  6:260796/135‑1 (MQ=255)
  gggCTGAAGATACCCTGCTGCCGACGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCgttgt                                                                                                                                    <  5:128367/135‑1 (MQ=255)
        aaGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTcccc                                                                                                                               <  7:86946/134‑1 (MQ=255)
         aGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTcccc                                                                                                                               <  3:178674/133‑1 (MQ=255)
         aGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTcccc                                                                                                                               <  3:184053/133‑1 (MQ=255)
         aGATACCCAGCTGCAGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGAAGGTGTTCATCACCTTCACCGGCGCGTTGTTcccc                                                                                                                               <  5:21256/133‑1 (MQ=255)
          gATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCATc                                                                                                                            >  2:46691/1‑135 (MQ=255)
                ctgctgCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGGTGTTCCCCATCCTGCTCa                                                                                                                     <  8:47399/136‑1 (MQ=255)
                 tgctgcCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCATCCTGCTCa                                                                                                                     <  4:262382/135‑1 (MQ=255)
                  gctgcCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTcccc                                                                                                                               <  7:221783/124‑1 (MQ=255)
                  gctgcCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTcccc                                                                                                                               >  8:221783/1‑124 (MQ=255)
                       cgccgcTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCATCCTGCTCAACACCGt                                                                                                              <  8:114451/136‑1 (MQ=255)
                                                   ggCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGGTGTTCCCCATCCTGCTCAACACCGTGCATGGCGTCGAGGCCGTCGACCCgcgc                                                                                  <  8:150913/136‑1 (MQ=255)
                                                       gtgGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGACCTGGCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCCTCCTGCTCCACACCGTGCATGGCGTCGCGGCCCGCGACCCCCGCGtgg                                                                              >  8:87238/1‑136 (MQ=255)
                                                           cGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGGTCCTCACCTTCACCGGCGCGGTGTTGCCCCTGCGGGGCCACACCGTGGATGGGGGGGCGGGCGGCGGCCCCCGCGtggtgg                                                                           >  5:172174/1‑135 (MQ=255)
                                                                  cccGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCATCCTGCTCAACACCGTGCATGGCGTCGAGGCCGTCGACCCGCGCTTGGTGGCATCGgc                                                                    <  3:253547/135‑1 (MQ=255)
                                                                  cccGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGGTTTTCCCCCGCCAGGGAACCACCCGGCATGGCGGCGAGGCCGGCGGCCCCCCCGTGGTGGGATCGgcg                                                                   >  7:258729/1‑136 (MQ=255)
                                                                       tGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCCTCCTGCTCAACCCCGTGCATGGCGTCGAGGCCGTCGCCCCGCGCGTGGTGGCATCGgcgcgccg                                                              >  8:81986/1‑134 (MQ=255)
                                                                             tCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCATCCTGCTCAACACCGTGCATGGCGTCGAGGCCGTCGACCCGCGCTTGGTGGCATCGGCGCGCAGCCTCg                                                         >  6:93606/1‑135 (MQ=255)
                                                                               cTGATGTTCCCGTCGTCGGAACTGTCGCGGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCATCCTGCTCCACACCGTGCATGGCGGCGAGGCCGTCGACCCGCGCTTGGGGGCATCGGCGCGCAGCCTCGGt                                                       <  3:266754/135‑1 (MQ=255)
                                                                               cTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCATCCTGCTCAACACCGTGCATGGCGTCGAGGCCGTCGACCCGCGCTTGGTGGCATCGGCGCGCAGCCTCGGt                                                       <  7:81986/135‑1 (MQ=255)
                                                                                   tGTTCCCGTCGGCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCATCCTGCTCAACACCGTGCATGGCGGCGAGGCCGTCGACCCGCGCTTGGTGGCATCGGCGCGCAGCCTCGGTGCCgg                                                  <  8:229555/136‑1 (MQ=255)
                                                                                    gTTCCCGTCGTCGGAACTGTCGATGCGGGTCATCACCTTCACCGGCGCGATGTTCCCCATCCTGCTCAACCCCGTGCATGGCGTCGAGGCCGTCGACCCGCGCTTGGTGGCATCGGCGCGCAGCCTCGGTGCCgg                                                  <  5:82164/135‑1 (MQ=255)
                                                                                        ccGTCGTCGGAACTGTCGATGGTGTGCATCACCTTCACCGGCGCGTTGTTCCCCATCCTGCTCAACACCGTGCATGGCGGCGAGGCCGTCGACCCGCGCTTGGTGGCATCGGCGCGCAGCCTCGGTGCCGGGCGCt                                             <  8:151060/136‑1 (MQ=255)
                                                                                          gtcgtcGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGGTGTTTCCCCTCCTGCTCAACCCCGTGCATGGCGTCGAGGCCCTCGCCCCCCGCTGGGTGGCATCGGCGCCCCGCCGCGGTGCCGGGCGCTg                                            >  5:119737/1‑135 (MQ=255)
                                                                                                 gAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCATCCTGCTCAACACCGTGCCTGGCGTCGAGGCCGTCGACCCGCGCTTGGTGGCATCGGCGCGCAGCCTCGGTGCCGGGCGCTGGGCGAt                                      <  6:207492/134‑1 (MQ=255)
                                                                                                     tGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCATCCTGCTCAACACCGTGCATGGCGTCGAGGCCGTCGACCCGCGCTTGGTGGCATCGGCGCGCAGCCTCGGTGCCGGGCGCTGGGCGATCCTgc                                 <  3:160011/135‑1 (MQ=255)
                                                                                                        cGATGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCATCCTGCTCAACACCGTGCATGGCGTCGAGGCCGTCGACCCGCGCTTGGTGGCATCGGCGCGCAGCCTCGGTGCCGGGCGCTGGGCGATCCTGcgcg                              <  3:197648/135‑1 (MQ=255)
                                                                                                          aTGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCATCCTGCTCAACACCGTGCATGGCGTCGAGGCCGTCGACCCGCGCTTGGTGGCATCGGCGCGCAGCCTCGGTGCCGGGCGCTGGGCGATCCTGCGCGAgg                           >  7:28245/1‑136 (MQ=255)
                                                                                                             gtgtTCATCACCTTCACCGGCGCGTTGTTCCCCATCCTGCTCAACACCGTGCATGGCGTCGAGGCCGTCGACCCGCGCTTGGTGGCATCGGCGCGCAGCCTCGGTGCCGGGCGCTGGGCGATCCTGCGCGAggtgg                        <  8:44743/136‑1 (MQ=255)
                                                                                                                   atcaCCTTCACCGGCGCGTTGTTCCCCATCCTGCTCAACACCGTGCATGGCGTCGAGGCCGTCGACCCGCGCTTGGTGGCATCGGCGCGCAGCCGCGGTGCCGGGCGCTGGGCCATCCTGCGCGAGGTGGTATTGc                  >  8:41013/1‑136 (MQ=255)
                                                                                                                   atcaCCTTCACCGGCGCGGTGTTGCCCCTCCGGGTCAACAACCTGGATGGCGTCGCGGGCCTCGCCCCCCCCTTGGTGGCATACGCGCGCACCCCCCGTGCCCGGCGCCGGGCGCGCCGGCGCGACGGGGttttt                   >  4:160011/1‑134 (MQ=255)
                                                                                                                    tcaCCTTCACCGGCGCGGTGTTCGCCATCCTGCTCAAAACCGTGCATGGCGTCGAGGCCGTCGAGCCGCGCTTGGTGGCATCGGCGCGCAGCCTCGGTGCCGGGCGCTGGGCGATCCTGCTCGAGGTGGTATTGc                  <  3:217553/135‑1 (MQ=255)
                                                                                                                             ccGGCGCGTTGTTACCCATCCAGCTCAACACCGAGCATGGCCTCGCGGCCGTCGACCCGCGCTTGGTGGCATCGGCGCGCAGCCTCGGGTCCGGGCGCGTGGCGCTACTGCGCCACGTGGGATTGGCTGGAGCGt         >  4:219526/1‑135 (MQ=255)
                                                                                                                                    gttgttCCCCATCCTGCTCAACACCGTGCATGGCGTCGAGGCCGTCGACCCGCGCTTGGTGGCATCGGCGCGCAGCCTCGGTGCCGGGCGCTGGGCGATCCTGCGCGAGGTGGTATTGCCTGGAGCGTTGCCGAg  <  5:93606/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
ATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACCTTCACCGGCGCGTTGTTCCCCATCCTGCTCAACACCGTGCATGGCGTCGAGGCCGTCGACCCGCGCTTGGTGGCATCGGCGCGCAGCCTCGGTGCCGGGCGCTGGGCGATCCTGCGCGAGGTGGTATTGCCTGGAGCGTTGCCGAG  >  KT2440/253211‑253477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑