| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,802,244 | T→A | 14.8% | intergenic (‑706/‑25) | CJ019_04509 ← / → dsbD_2 | hypothetical protein/Thiol:disulfide interchange protein DsbD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,802,244 | 0 | T | A | 14.8% | 59.3 / 5.8 | 27 | intergenic (‑706/‑25) | CJ019_04509/dsbD_2 | hypothetical protein/Thiol:disulfide interchange protein DsbD |
| Reads supporting (aligned to +/- strand): ref base T (15/8); new base A (2/2); total (17/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-01 | |||||||||||
GCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAACTCTTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACTGGCCGGCCCGTTGCAAGCCAACCCGTTCGATGTGAAACCAGACTTCCTGCCGGTTGACCAAGCCTTCGT > KT2440/4802121‑4802372 | gccTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAACTCTTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTg < 5:175697/135‑1 (MQ=255) aacaacaaAGACCCTGCAAACCCAGAACAAACTCTTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATgc < 6:65210/134‑1 (MQ=255) ccAGAACAAACTCTTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTcac < 4:258807/135‑1 (MQ=255) aGAACAAACTCTTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACt > 8:45433/1‑136 (MQ=255) aaCAAACTCTTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTc > 4:25355/1‑135 (MQ=255) cAAACTCTTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCAATCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTc < 8:173679/127‑1 (MQ=255) cAAACTCTTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCAATCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTc < 8:169366/127‑1 (MQ=255) cAAACTCTTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCAATCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTc > 7:173679/1‑127 (MQ=255) cAAACTCTTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCAATCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTc > 7:169366/1‑127 (MQ=255) cAAACTCTTACTACTCCTGACCAACCCCCAACACCCGCCTTAAACTTCATTAAATCAAACCCAGCCAACATGGCCACATTCACAGATCTGCCAAGGCGAACACATTCTCGTTCACCTGCTCATCCAAACACTCCt > 6:101768/1‑135 (MQ=255) ctTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACTggc > 1:94838/1‑135 (MQ=255) gACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACTGGCCGGCCCGTTGc > 7:199905/1‑134 (MQ=255) cacccCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGcgcg > 6:54522/1‑88 (MQ=255) cacccCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGcgcg < 5:54522/88‑1 (MQ=255) ccAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACTGCCCGGCCCGTTGCAAGCCAACCCg > 7:95558/1‑136 (MQ=255) cacCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACTGGCCGGCCCGTTGCAAGCCAAccc < 7:105710/131‑1 (MQ=255) cacCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACTGGCCGGCCCGTTGCAAGCCAAccc > 8:105710/1‑131 (MQ=255) acCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTc < 2:162093/95‑1 (MQ=255) acCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTc > 1:162093/1‑95 (MQ=255) acCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACTGGCCGGCCCGTTGCAAGCCAACCCGTTCg > 6:216176/1‑135 (MQ=255) ttAATCAACCCCAGCCAGCATGGCCCCATTCCCAGAACTGCCAAGGAGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCAACAGTCCGGCCCGTTGCAAGCCAACCCGTTAGATGTTAAACCAGCCTTCCt > 7:257976/1‑136 (MQ=255) aCCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACTGGCCGGCCCGTTGCAAGCCAACCCGTTCGATGTGAAACCAGACTTCCTGCCGGt > 5:77574/1‑135 (MQ=255) ccagcctgCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCAGACTCCTACTGGGCGGCCCGTTGCTAGCCATCCCGTTCGATGTGAAACGAGACTTCCTGCCGGTTGa < 5:101768/135‑1 (MQ=255) cagcATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACTGGCCGGCCCGTTGCAAGCCAACCCGTTCGATGTGAAACCAGACTTCCTGCCGGTTGACCAAg < 3:25355/135‑1 (MQ=255) agcATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACTGGCCGGCCCGTTGCAAGCCAACCCGTTCGATGTGAAACCAGACTTCCTGCCGGTTGACCAAGcc > 8:34374/1‑136 (MQ=255) ggCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACTGGCCGGCCCGTTGCAAGCCACCCCGTTCGATGTGAAACCAGACTTCCTGCCGGTTGACCAAGCCTTCGt > 8:277863/1‑136 (MQ=255) ggCCCCATTCCCAGATCAGCCAAGCCGAAGACATGCGCGTTCTCCTGCTCTTCCACACACTCCTACTGGCCTCCCCGATGCACGCCAACCCGTACGATGTGAAACCAGACTTCCTGCCGGTTGACCAAGccaacg > 4:229175/1‑131 (MQ=255) | GCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAACTCTTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACTGGCCGGCCCGTTGCAAGCCAACCCGTTCGATGTGAAACCAGACTTCCTGCCGGTTGACCAAGCCTTCGT > KT2440/4802121‑4802372 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |