Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,528,483 A→T 7.3% intergenic (‑183/‑330) braC_2 ← / → pncB2 Leucine‑, isoleucine‑, valine‑, threonine‑, and alanine‑binding protein/Nicotinate phosphoribosyltransferase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,528,4830AT 7.3% 151.8 / 4.1 55intergenic (‑183/‑330)braC_2/pncB2Leucine‑, isoleucine‑, valine‑, threonine‑, and alanine‑binding protein/Nicotinate phosphoribosyltransferase 2
Reads supporting (aligned to +/- strand):  ref base A (26/25);  new base T (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.82e-01

CGGTACTCAGAGCAAGGGCCGTGCCATCGGTTTTGTCTTCCGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTGTAACAGCAGACGTCACCGAACTGCACACCGCTGGTGCGCGACTGCTACAGGCGGC  >  KT2440/5528350‑5528609
                                                                                                                                     |                                                                                                                              
cGGTACTCAGAGCAAGGGCCGTGCCATCGGTTTTGTCTTCCGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCCCCCCACg                                                                                                                              >  7:62603/1‑136 (MQ=255)
cGGTACTCAGAGCAAGGGCCGTGCCATCGGTTTTGTCTTCCGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCacac                                                                                                                               <  5:272117/135‑1 (MQ=255)
 ggTACTCAGAGCAAGGGCCGTGCCATCGGTTTTGTCTTCCGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCGTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCCCACg                                                                                                                              >  8:251118/1‑135 (MQ=255)
      tCAGAGCAAGGGCCGTGCCATCGGTTTTGTCTTCCGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACCGACAg                                                                                                                         >  1:176692/1‑135 (MQ=255)
           gCAAGGGCCGTGCCATCGGTTTTGTCTTCCGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCCCCACACGTACAGACaa                                                                                                                     >  6:48911/1‑134 (MQ=255)
            cAAGGGCCGTGCCATCGGTTTTGTCTTCCGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACAGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAg                                                                                                                    <  4:163168/134‑1 (MQ=255)
                ggCCGTGCCATCGGTTTTGTCTTCCGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCaa                                                                                                              >  7:30832/1‑136 (MQ=255)
                  ccGTGCCATCGGTTTTGTCTTCCGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCGTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCCCACGTACCGACAAGGCGCAAc                                                                                                             >  6:38040/1‑135 (MQ=255)
                   cGTGCCATCGGTTTTGTCTTCCGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAAcc                                                                                                            <  2:76384/135‑1 (MQ=255)
                   cGTGCCATCGGTTTTGTCTTCCGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAAcc                                                                                                            <  1:87548/135‑1 (MQ=255)
                      gCCATCGGTTTTGACTTCCGGCAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTc                                                                                                         <  5:162674/135‑1 (MQ=255)
                       ccATCGGTTTTGTCTTCCGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGt                                                                                                          >  6:222407/1‑133 (MQ=255)
                          tCGGTTTTGTCTTCCGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGt                                                                                                     <  4:3654/135‑1 (MQ=255)
                             gTTTTGTCTTCCGGGAAAACACTCTGTGAAGGGTGCCGGTCGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGc                                                                                                   <  6:238820/134‑1 (MQ=255)
                                  gTCTTCCGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCcatca                                                                                              <  3:123629/134‑1 (MQ=255)
                                       ccGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCa                                                                                         <  7:262614/134‑1 (MQ=255)
                                        cGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCa                                                                                         <  5:107793/133‑1 (MQ=255)
                                         gggAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCGTTTCTTTATTTAGCGTGCAAACCTCTGCCACCCCGCCTGCGCCACCCCACCTACAGACAAGGAGCAACCGTCCAGTGCCATCATAGCAAcc                                                                                      >  2:201247/1‑135 (MQ=255)
                                           gAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTGTTTATTGAGCGTTCAACAGTCTGACCCGCGGCAGGCGCCACCACACGTACAGACAAGGCGCAACCGGCGAGTGCCATCATTTCAAccca                                                                                    >  5:199078/1‑134 (MQ=255)
                                           gAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCATTTGGTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGAACAGGAGCAACCGTCGAGTGCCATCATTGCAAccca                                                                                    >  2:241739/1‑134 (MQ=255)
                                             aaaCACACTGTGAAGGGGGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTgg                                                                                  <  1:204281/135‑1 (MQ=255)
                                                cacacTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGt                                                                                                          <  3:5054/108‑1 (MQ=255)
                                                cacacTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGt                                                                                                          >  4:5054/1‑108 (MQ=255)
                                                    cTGTGAAGGGTGCCGGGAGGCGACGGGCGGCCTTTTCTTTATTGCGCGGGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAgtgt                                                                           <  4:142108/135‑1 (MQ=255)
                                                        gAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTAc                                                                       >  2:18923/1‑135 (MQ=255)
                                                        gAAGGGTGCCGGTAGGCGACGGGCGGCCGTTTGGTTATTTATCGTGCAACCCTCGGCCACCCCGCCCGCGGCCCCAAACGTACAGACCAGGACCAACCCGCCACTGGCCAAATTGCCACCCGGGCAACTTTTTaa                                                                       >  6:155269/1‑134 (MQ=255)
                                                           gggTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGc                                                                   <  7:224420/136‑1 (MQ=255)
                                                             gTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGAGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGCGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCaa                                                                 >  7:1526/1‑136 (MQ=255)
                                                                ccGGTAGGCGACGGTCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCa                                                                                         <  7:89564/109‑1 (MQ=255)
                                                                ccGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAAcc                                                               <  1:201247/135‑1 (MQ=255)
                                                                ccGGTAGGAGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCa                                                                                         >  8:89564/1‑109 (MQ=255)
                                                                 cGGTAGGCGACGGGCGGCCTTTTGTTTATTGAGCGTGCCACCGTCTGCCCCGCGGCAGGCGCCACCCCACGGACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCg                                                              >  1:71178/1‑135 (MQ=255)
                                                                  ggTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCCCGCGGCAGGCGCCACCACACGTACAGCCAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGcc                                                            >  8:76037/1‑136 (MQ=255)
                                                                  ggTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACTCGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGc                                                             >  3:219049/1‑135 (MQ=255)
                                                                  ggTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACTCGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGc                                                             >  3:215272/1‑135 (MQ=255)
                                                                  ggTAGGCGACGGGCGGCCGTTTCTTTATTGAGCGTGCCACCGTCTGCCCCGCGGCACGCGCCACCCCACGTACAGACCAGGAGCACCCGGCGAGTGCCATCATGGCAACCCTGGCAAGTGTTTAAGTGCAACCGc                                                             >  3:72131/1‑135 (MQ=255)
                                                                    tAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTTCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGccc                                                           <  2:147565/135‑1 (MQ=255)
                                                                       gCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACTCGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTg                                                         <  4:219049/134‑1 (MQ=255)
                                                                       gCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACTCGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTg                                                         <  4:215272/134‑1 (MQ=255)
                                                                         gACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTg                                                                                                    <  1:208665/89‑1 (MQ=255)
                                                                         gACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTg                                                                                                    >  2:208665/1‑89 (MQ=255)
                                                                           cGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTGTAAca                                                    >  3:136429/1‑135 (MQ=255)
                                                                                  ccTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTGTAACAGCAGACg                                             <  6:256887/135‑1 (MQ=255)
                                                                                  ccTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTGTAACAGCAGACg                                             <  1:83258/135‑1 (MQ=255)
                                                                                  ccTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTGTAACAGCAGACg                                             <  5:67574/135‑1 (MQ=255)
                                                                                       tCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTGTAACAGCAGACGTCa                                          <  2:189399/133‑1 (MQ=255)
                                                                                             ttGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTGTAACAGCAGACGTCACCGAACt                                   <  1:18923/134‑1 (MQ=255)
                                                                                                aGCGTGCAACCGTCTGCCCCGCGGCAGGCGCCACCCCACGTTCAGACAAGGAGCAACCGGCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTGTAACAGCAGACGTCACCGAACTGca                                <  6:256431/134‑1 (MQ=255)
                                                                                                   gTGCAACCGTCTGCCACGCGTCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTGTAACAGCAGACGTCACCGAACTGCTCAcc                            >  3:156355/1‑135 (MQ=255)
                                                                                                     gCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCATGTGTTTACGTGCAACCGCCCTGTAACAGCAGACGTCACCGAACTGCACACCGc                          >  6:95196/1‑135 (MQ=255)
                                                                                                     gCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTGTAACAGCAGACGTCACCGAACTGCACACCGc                          >  3:62307/1‑135 (MQ=255)
                                                                                                     gCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTGTAACAGCAGACGTCACCGAACTGCACACCGc                          >  1:152061/1‑135 (MQ=255)
                                                                                                       aaCCGTCTGCCACGCGGCAGGCGCCAACACACGTACAGAAACGTAGCAACCGTCGAGTCCCATCATTGCAACCCTGGCAAGTGTTTAGGTGCAACCGCACTTTAACAGCAGAGGTCACCTAACTCCACACCGCTg                        <  4:231504/135‑1 (MQ=255)
                                                                                                             cTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTGTAACAGCAGACGTCACCGAACTGCACACCGCTGGTgcgc                  >  4:106869/1‑135 (MQ=255)
                                                                                                                   cgcgGCAGGCGCCACCACACGTACAGACACGCCGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTGTAACAGCCGACGTCACCGAACTGCACACCGCTGGTGCGCGACTGc            <  5:38040/135‑1 (MQ=255)
                                                                                                                             gcCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTGTAACAGCAGACGTCACCGAACTGCACACCGCTGGTGCGCGACTGCTACAggcggc  >  4:145370/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                              
CGGTACTCAGAGCAAGGGCCGTGCCATCGGTTTTGTCTTCCGGGAAAACACACTGTGAAGGGTGCCGGTAGGCGACGGGCGGCCTTTTCTTTATTGAGCGTGCAACCGTCTGCCACGCGGCAGGCGCCACCACACGTACAGACAAGGAGCAACCGTCGAGTGCCATCATTGCAACCCTGGCAAGTGTTTACGTGCAACCGCCCTGTAACAGCAGACGTCACCGAACTGCACACCGCTGGTGCGCGACTGCTACAGGCGGC  >  KT2440/5528350‑5528609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑