Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,582,884 G→A 12.5% T31T (ACC→ACT)  parE ← DNA topoisomerase 4 subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,582,8840GA12.5% 88.9 / 4.7 32T31T (ACC→ACT) parEDNA topoisomerase 4 subunit B
Reads supporting (aligned to +/- strand):  ref base G (12/16);  new base A (2/2);  total (14/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

CGGCATGCAGGATGACCTGCACCGAACGCGCGTGGCCGGCCAGTGCTTCGTCGACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTGGGATTGGCCATAGGGTCTCGTCGTCAGTCTGGTGAATGCAAAAAAGTCA  >  KT2440/5582781‑5583014
                                                                                                       |                                                                                                                                  
cGGCATGCAGGATGACCTGCACCGAACGCGCGTGGCCGGCCAGTGCTTCGTCGACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGAt                                                                                                     <  1:97766/135‑1 (MQ=255)
            tGACCTGCACCGAACGCGCGTGGCCGGCCAGTGCTTCGTCGACACTGTTGTCGATGACTTCCTGGGCCAGGTGGGTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCgaga                                                                                         <  3:237114/135‑1 (MQ=255)
                  gCACCGAACGCGCGTGGCCGGCCAGTGCTTCGTCGACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACtt                                                                                   >  5:251847/1‑135 (MQ=255)
                  gCACCGAACGCGCGTGGCCGGCCAGTGCTTCGTCGACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCACGAGAGGACtt                                                                                   >  4:127850/1‑135 (MQ=255)
                   cACCGAACGCGCGTGGCCGGCCAGTGCTTCGTCGACACTGTTGTTGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTc                                                                                  <  2:169181/135‑1 (MQ=255)
                   cACCGAACGCGCGTGGCCGGCCAGTGCTTCGTCGACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTc                                                                                  <  7:65180/135‑1 (MQ=255)
                       gAACGCGCGTGGCCGGCCAGTGCTTCGTCGACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATg                                                                              >  4:127337/1‑135 (MQ=255)
                        aaCGCGCGTGGCCGGCCAGTGCTTCGTCGACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGc                                                                            <  8:11392/136‑1 (MQ=255)
                           gcgcgTGGCCGGCCAGTGCTTCGTCGACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGCCCGGATCAAGGCCCGAGAGGCCTTCGATGGCGTc                                                                         >  8:203911/1‑136 (MQ=255)
                           gcgcgTGGCCGGCCAGTGCTTCGTCGACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGt                                                                          <  1:153986/135‑1 (MQ=255)
                           gcgcgTGGCCGGCCAGTGCTTCGTCGACACTGTTGTCGATGACTCCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTc                                                                         >  8:139412/1‑136 (MQ=255)
                                tGGCCGGCCAGTGCTTCGTCGACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCAGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGt                                                                    >  8:66884/1‑136 (MQ=255)
                                tGGCCGGCCAGTGCTTCGTCGACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCAGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGt                                                                    >  8:63093/1‑136 (MQ=255)
                                  gccgggcAGTGCTTCGTCGACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGtt                                                                   <  5:123349/135‑1 (MQ=255)
                                            gCTTCGTCGACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTa                                                         <  2:54165/135‑1 (MQ=255)
                                                 gtcgACACTGTTGTCGATGACTCCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTg                                                   <  7:139412/136‑1 (MQ=255)
                                                  tcgACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTGGGGGCGGCTGGGATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTg                                                   <  1:231715/135‑1 (MQ=255)
                                                   cgACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGGGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGGTTTAGGGGCTAgcggggg                                                  >  1:134566/1‑131 (MQ=255)
                                                   cgACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTgg                                                  <  1:293/135‑1 (MQ=255)
                                                   cgACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTgg                                                  <  4:30186/135‑1 (MQ=255)
                                                   cgACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCGTGCGGACCGGCTAAAGGCCCGAGCGGACTTCGATGGCGGCTGCGGTATAGGGGCTAGCGCggg                                                  >  1:199510/1‑135 (MQ=255)
                                                        cTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTGGGATTgg                                            >  7:165076/1‑136 (MQ=255)
                                                         tgttgtCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTGGGATTgg                                            <  3:127337/135‑1 (MQ=255)
                                                                    aCTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCAGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTGGGATTGGCCATAGGGtct                                 <  7:63093/135‑1 (MQ=255)
                                                                    aCTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCAGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTGGGATTGGCCATAGGGtct                                 <  7:66884/135‑1 (MQ=255)
                                                                      ttCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTGGGATTGGCCATAGGGTCtcgt                              >  7:275827/1‑136 (MQ=255)
                                                                         cTGGGCCAGGTGGTTGGGGCGGCTGGGATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTGGGATTGGCCATAGGGTCtcgt                              <  4:96656/133‑1 (MQ=255)
                                                                                     gtTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTGGGATTGGCCATAGGGTCTCGTCGTCAGTCTGGTGaa               >  7:195529/1‑136 (MQ=255)
                                                                                         gggCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTGGGATTGGCCATAGGGTCTCGTCGTCAGTCTGGTGAATg             >  5:60072/1‑134 (MQ=255)
                                                                                                  gTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTGGGATTGGCCATAGGGTCTCGTCGTCAGTCTGGTGAATGCAAAAAAGt    >  2:189413/1‑134 (MQ=255)
                                                                                                  gTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTGGGATTGGCCATAGGGTCTCGTCGTCAGTCTGGTGAATGCAAAAAAGTCa  <  8:195529/136‑1 (MQ=255)
                                                                                                  gTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTGGGATTGGCCATAGGGTCTCGTCGTCAGTCTGGTGAATGCAAAAAAGTCa  <  8:49444/136‑1 (MQ=255)
                                                                                                       |                                                                                                                                  
CGGCATGCAGGATGACCTGCACCGAACGCGCGTGGCCGGCCAGTGCTTCGTCGACACTGTTGTCGATGACTTCCTGGGCCAGGTGGTTGGGGCGGCTGGTATCGGTGTACATGCCCGGCCGCTTGCGGACCGGATCAAGGCCCGAGAGGACTTCGATGGCGTCTGCGTTATAGGCGCTAGCGCTGGGATTGGCCATAGGGTCTCGTCGTCAGTCTGGTGAATGCAAAAAAGTCA  >  KT2440/5582781‑5583014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑