| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,791,128 | G→A | 19.8% | P203S (CCA→TCA) | gltB ← | Glutamate synthase [NADPH] large chain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,791,128 | 0 | G | A | 19.8% | 40.1 / 4.2 | 24 | P203S (CCA→TCA) | gltB | Glutamate synthase [NADPH] large chain |
| Reads supporting (aligned to +/- strand): ref base G (7/10); new base A (2/2); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
GTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGG > KT2440/5791012‑5791246 | gTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATc > 3:11201/1‑135 (MQ=255) tgGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGGTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATcgcgc < 8:32655/135‑1 (MQ=255) aGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGcatc > 6:129524/1‑135 (MQ=255) gAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGAATAAAACGCCGCGAGATCGCGCGGcatca > 4:82318/1‑134 (MQ=255) gAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGAATAAAACGCCGCGAGATCGCGCGGcatca > 4:78384/1‑134 (MQ=255) aGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAg > 8:64679/1‑136 (MQ=255) gcgcCTGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGAGCGGGGTTTGCAGGCGCTCGTCACCCTGGTCTGTATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAga < 5:266185/135‑1 (MQ=255) gcCAGGGGCCATTTCGGCAGGGTGTGGGTGGAGAAGCGCGGGTGGAACACGCAGATCGCGGTTTGCAGGCGCGCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAga < 2:14809/133‑1 (MQ=255) aGCGGCAATTTCGGCAGGGTGTGGGTGGAGAAGCGCTGGTGTAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGatgatg < 4:97305/135‑1 (MQ=255) aGCGGCAATTGCGGCTGGGTGTGGGTGGACACGGGGTGGTGCAACACGCAGAGCGGGGTTTGCAGGCGCGCGCCCCCCTGGTCTGCAAAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGatgatg < 2:271411/135‑1 (MQ=255) tgcGGCTGGGGGTTGGGGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCCGGCGCTCGTCACCCAGGTCTGGAAAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGc < 3:28453/133‑1 (MQ=255) cGGCTGGGGGTTGGGGGAGAAGGGGTGGTGGAACACGCTGATCGGGGTTTGCAGGCGCTCGTCACCCTGGTCTGTAAAAACCGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCg < 5:268145/134‑1 (MQ=255) gCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCgaga < 4:178611/135‑1 (MQ=255) aGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGAGCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAg < 4:218342/135‑1 (MQ=255) aGGGTGTTGGGGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCGCGTCACCCAGGGCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAg < 2:26095/135‑1 (MQ=255) gtggtggtgGAGAAGCGCTGGGGGAACACGCAGATCGCGGTTTGCAGGCGCTCGGCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGc < 2:163163/133‑1 (MQ=255) tggAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATg > 8:154320/1‑134 (MQ=255) gaAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAACACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTg < 3:206880/135‑1 (MQ=255) gcgcTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGGCTTGTGCGAGACGCTGCAGATGTAGTGGTc > 6:209448/1‑135 (MQ=255) ggtggAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGAATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGcgt < 3:82318/135‑1 (MQ=255) ggtggAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGAATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGcgt < 3:78384/135‑1 (MQ=255) cTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTCCGAGACGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGACCGCCGACgg > 7:22214/1‑136 (MQ=255) gTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCg < 7:167903/74‑1 (MQ=255) gTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCg > 8:167903/1‑74 (MQ=255) | GTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGG > KT2440/5791012‑5791246 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |