Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 6,014,104 C→T 5.9% G127D (GGC→GAC)  dadA1_4 ← D‑amino acid dehydrogenase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,014,1040CT 5.9% 221.5 / 3.2 68G127D (GGC→GAC) dadA1_4D‑amino acid dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base C (31/33);  new base T (2/2);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

CGAGCAGCTCGTAGGGCACACCGGACTGTTCGAGTACGGCAATGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCTGTGCCATCCACA  >  KT2440/6013982‑6014224
                                                                                                                          |                                                                                                                        
cGAGCAGCTCGTAGGGCACACCGGACTGTTCGAGTACGGCAATGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGgcgcg                                                                                                             <  7:172872/136‑1 (MQ=255)
          gTAGGGCACACCGGACTCTTCGAGTACGGCAATGTCATTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTCGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCCGCtcgtc                                                                                                    >  7:11298/1‑135 (MQ=255)
            aGGGCACACCGGACTGTGCGAGTACGGCAATGGCCTTGGCCGCAGCATCGACGGGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGa                                                                                                  <  5:26882/135‑1 (MQ=255)
               gCACACCGGACTTTTAGAGTAGGGCAATGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGGGCGGAACAGCTGGGTGGTGCCCTGGCTGCGGTTTTCGTAGTTGATGCCGGTTGCGGCGCGCAGCTCGTCGAGGc                                                                                               <  2:46590/135‑1 (MQ=255)
                    ccGGACTGTTCGAGTACGGCAATGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCAc                                                                                         >  7:240707/1‑136 (MQ=255)
                     cGGACTGTTCGAGTACGGCAATGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGGTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACg                                                                                        <  8:130991/136‑1 (MQ=255)
                     cGGACTGTTCGAGTACGGCAATGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCAc                                                                                         <  1:169583/135‑1 (MQ=255)
                     cGGACTGTTCGAGTACGGCAATGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACg                                                                                        <  8:219826/136‑1 (MQ=255)
                      ggACTGTTCGAGTACGGCAATGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACg                                                                                        >  5:256449/1‑135 (MQ=255)
                           gTTCGAGTACGGCAATGTCCTTGGCCGCAGCATCGCCCTGGGCCTGGGTGCGGAACAGCTGGGGGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCCGCTCGTCGAGGCAGTCACGGCTGt                                                                                   >  6:86592/1‑135 (MQ=255)
                           gTTCGAGTACGGCAATGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGTCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCa                                                                                          <  4:171710/128‑1 (MQ=255)
                           gTTCGAGTACGGCAATGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGTCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCa                                                                                          >  3:171710/1‑128 (MQ=255)
                           gTTCGAGTACGGCAATGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGt                                                                                   >  2:253535/1‑135 (MQ=255)
                             tCGAGTACGGCAATGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTAc                                                                                 <  5:102551/135‑1 (MQ=255)
                               gAGTACGGCAATGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTc                                                                               >  6:7169/1‑135 (MQ=255)
                               gAGTACGGCAATGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCg                                                                              <  8:266417/136‑1 (MQ=255)
                                      gCAATGTCCTTGGCCGCACCCTCGACCGGGGCCTGGGTGCGGAACAGCGGGGTGGGGCCCAGGCGGCGGTTGTCGTAGTTGATGCCGGTGTCGGCGCGCAGCGCGGCGAGGCAGTCACGGCTGTACTCGGACAgg                                                                        <  5:5707/135‑1 (MQ=255)
                                         aTGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCTGACAGgcgc                                                                     >  6:160561/1‑135 (MQ=255)
                                         aTGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGgcgc                                                                     >  6:258062/1‑135 (MQ=255)
                                          tGTCCTTGGCCGCAGCATCGCCCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCa                                                                    >  6:249382/1‑135 (MQ=255)
                                            tCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCa                                                                 <  8:255959/136‑1 (MQ=255)
                                               ttGGCCGCAGCATCGACCTGGGCCGGGGTGAGGAACAGCTGGGGGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATg                                                               <  5:55394/135‑1 (MQ=255)
                                                  gCCGCAGCATCGCCCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATgcgc                                                            <  4:198347/135‑1 (MQ=255)
                                                  gCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATgcgc                                                            >  1:201099/1‑135 (MQ=255)
                                                    cGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTc                                                          >  5:274399/1‑135 (MQ=255)
                                                     gcagcaTCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCt                                                        >  8:38179/1‑136 (MQ=255)
                                                         caTCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCTGTCACGGCTGTACTCGGCCAGGCGCACCAGGCGGTCCTTGt                                                     >  5:32275/1‑135 (MQ=255)
                                                         caTCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGt                                                     >  5:108023/1‑135 (MQ=255)
                                                         caTCGACCTGGGCCTGGGTGAGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGtt                                                    >  7:260504/1‑136 (MQ=255)
                                                          aTCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTc                                                   >  7:53980/1‑136 (MQ=255)
                                                            cGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACCGGCGCACCATGCGCTCCTTGTTCa                                                  >  4:142017/1‑135 (MQ=255)
                                                                      ctgggTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGgc                                                                                                                <  4:115160/63‑1 (MQ=255)
                                                                      ctgggTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGgc                                                                                                                >  3:115160/1‑63 (MQ=255)
                                                                      ctgggTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCgg                                       >  8:123653/1‑136 (MQ=255)
                                                                      ctgggTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCgg                                       >  7:232532/1‑136 (MQ=255)
                                                                      ctgggTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATCCGATCCTTGTTCACCGCGTAGCg                                        >  2:77521/1‑135 (MQ=255)
                                                                       tgggTGCGGAACAGCTGGGTGGTGCCCAGGCGGCGGTTTTCGTAGTTGATGCCGGTTGCGGCGCGCAGCTCGGCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGc                                      <  8:118006/136‑1 (MQ=255)
                                                                            gCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGTCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTg                                    >  6:83434/1‑133 (MQ=255)
                                                                            gCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGgca                                 <  7:123653/136‑1 (MQ=255)
                                                                             cGGAACAGCTGGGTGGTGCCCAGGCTGGGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGAAGCGCGGCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGgca                                 <  5:258062/135‑1 (MQ=255)
                                                                                aaCAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGgcagtg                              <  6:108023/135‑1 (MQ=255)
                                                                                aaCAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGgcagtg                              <  6:274399/135‑1 (MQ=255)
                                                                                 aCAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGgcagtg                              >  8:181770/1‑134 (MQ=255)
                                                                                      tgggtgggGCCCTGGCGGCGGTTTTCGTAGTTGAGGCCGGTTTCGGCGCGCAGCGCGGCGAGGCAGGCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTg                        <  5:7169/135‑1 (MQ=255)
                                                                                      tGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTgc                       >  8:18907/1‑136 (MQ=255)
                                                                                        ggtggtGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCtcgtc                                                                                                    >  1:260665/1‑57 (MQ=255)
                                                                                        ggtggtGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCtcgtc                                                                                                    <  2:260665/57‑1 (MQ=255)
                                                                                        ggtggtGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTgcg                      >  8:98187/1‑135 (MQ=255)
                                                                                            gtgCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCgcagca                 <  7:98187/136‑1 (MQ=255)
                                                                                             tgCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCgcagca                 >  1:146808/1‑135 (MQ=255)
                                                                                               cccAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCt              <  8:260504/136‑1 (MQ=255)
                                                                                                ccAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGt                                                     >  1:2886/1‑96 (MQ=255)
                                                                                                ccAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGt                                                     <  2:2886/96‑1 (MQ=255)
                                                                                                ccAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCtg             <  8:226065/136‑1 (MQ=255)
                                                                                                ccAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCtg             >  8:200314/1‑136 (MQ=255)
                                                                                                ccAGGCTGCGGTTTTCGTAGTTGATGCCGGTTGCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCt              <  5:209314/135‑1 (MQ=255)
                                                                                                ccAGGCTGCGGTTTGCGTAGTGGCTGCCGGTGTCGGCGCGCTGCTCGGCGAGGCAGTCACGGCTGTACGCGGACAGGCGCACCATGCGCGCCTTGTTCCCCGCGTCGCGGCTGGCAGTGCAGTTGCGCAGCATCt              <  5:86592/135‑1 (MQ=255)
                                                                                                 cAGGCTGCGGTTTTCGTAGTTGATGCCGGTTGCGGCGCGCAGCGCGGCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCtg             <  6:251714/135‑1 (MQ=255)
                                                                                                  aGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCtgt            >  6:225536/1‑135 (MQ=255)
                                                                                                   ggcggcGGTTTTGGTTGTTGAGGCCGTTTGCGGCGCGCAGGTGGTGGTGGCAGCCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCtgtg           <  2:132396/135‑1 (MQ=255)
                                                                                                   ggcggcGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCtgtg           <  6:16911/135‑1 (MQ=255)
                                                                                                   ggCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCtgtg           <  3:248248/135‑1 (MQ=255)
                                                                                                   ggCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCtgtg           <  6:256449/135‑1 (MQ=255)
                                                                                                       gCGGTTTTCGTAGTTGATGTCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCTGTGCCAt       <  5:83434/135‑1 (MQ=255)
                                                                                                       gCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCTGTGCCAt       <  1:253535/135‑1 (MQ=255)
                                                                                                           ttttCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCTGTGCCATcc     >  4:140796/1‑133 (MQ=255)
                                                                                                           ttttCGTAGTTGATGCCGGTTGCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCTGTGCCATCcac   <  3:144228/135‑1 (MQ=255)
                                                                                                            tttCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCTGTGCCATCcaca  >  2:146560/1‑135 (MQ=255)
                                                                                                                          |                                                                                                                        
CGAGCAGCTCGTAGGGCACACCGGACTGTTCGAGTACGGCAATGTCCTTGGCCGCAGCATCGACCTGGGCCTGGGTGCGGAACAGCTGGGTGGTGCCCAGGCTGCGGTTTTCGTAGTTGATGCCGGTTTCGGCGCGCAGCTCGTCGAGGCAGTCACGGCTGTACTCGGACAGGCGCACCATGCGCTCCTTGTTCACCGCGTAGCGGCTGGCAGTGCAGTTGCGCAGCATCTGTGCCATCCACA  >  KT2440/6013982‑6014224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑