Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 6,021,216 A→G 8.2% F159S (TTC→TCC)  ycaD_4 ← putative MFS‑type transporter YcaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24406,021,2160AG 8.2% 143.2 / 3.2 49F159S (TTC→TCC) ycaD_4putative MFS‑type transporter YcaD
Reads supporting (aligned to +/- strand):  ref base A (14/30);  new base G (2/2);  total (17/32)
Fisher's exact test for biased strand distribution p-value = 5.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTT  >  KT2440/6021084‑6021340
                                                                                                                                    |                                                                                                                            
gCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAg                                                                                                                            >  3:102611/1‑135 (MQ=255)
     tACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGaa                                                                                                                       <  3:46021/135‑1 (MQ=255)
     tACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGaa                                                                                                                       <  5:150856/135‑1 (MQ=255)
     tACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGaa                                                                                                                       <  3:123992/135‑1 (MQ=255)
        cGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAgtcg                                                                                                                   >  7:237604/1‑136 (MQ=255)
          gCAAGCGTCGGCAGAAGGCGAACAGGGCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAgtcgt                                                                                                                  <  3:81393/135‑1 (MQ=255)
             aGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGtca                                                                                                                >  6:115954/1‑134 (MQ=255)
             aGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCAGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGtca                                                                                                                >  1:235455/1‑134 (MQ=255)
               cggcGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGtcatca                                                                                                             <  2:97598/132‑1 (MQ=255)
                      aGAAGGCTACCAGGGCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGAAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGCCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGa                                                                                                      <  1:202339/135‑1 (MQ=255)
                                       ccAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAAcg                                                                                    <  7:85393/136‑1 (MQ=255)
                                        cAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGATGAAACCCAGCACCAAcg                                                                                    >  4:222162/1‑135 (MQ=255)
                                               gACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAAcggcccggc                                                                             <  5:115954/135‑1 (MQ=255)
                                               gACACTGCAGGCTGCGGGGCGGGGGGCTCCACCACGCCCCAGCACAACCCGCGGCGCGGCCAGCTGCAGGCCGGGCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCTAGGAAACCCAGCACCAAcggcccggc                                                                             <  5:24103/135‑1 (MQ=255)
                                                ccacTGCAGGCTTCCGTGCGCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAAcggcccggcc                                                                            <  4:252535/134‑1 (MQ=255)
                                                     gccggCTTCCGTGCTCGGCGCGCCACCACCCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGGACCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTg                                                                       <  1:112447/132‑1 (MQ=255)
                                                     gCAGGCTTCCGGGCGCGGCGCGCCACCCCGCCCCAGCAGAACCACGGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGGAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTg                                                                       <  1:108953/135‑1 (MQ=255)
                                                      cAGGCTTCCGTGCGCGGCGCTCCACCACGCCCCAGCAGAACCCGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTgg                                                                      <  4:235330/135‑1 (MQ=255)
                                                      cAGGCTGCCGTGCGCGGCGCTCCACCACGCCCCAGCAGAACCCGCGGCGCGGCCAGCAGCAGGCCGGGCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTg                                                                       <  7:243428/134‑1 (MQ=255)
                                                       aGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTg                                                                       <  4:102328/133‑1 (MQ=255)
                                                       aGGCGTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGACCCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTg                                                                       <  5:223585/133‑1 (MQ=255)
                                                         gCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGGAGCCGAAGTCGTCATCAGAGCCGAGGCAACCCAGCCCCCACCGCCCCGCCAACTGGcac                                                                   >  2:108953/1‑133 (MQ=255)
                                                         gCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGGAGCCGAACTCGTCATCAGAGCCGAGGAAACCCACCACCAACCGCCCGGCCAACAGGCTc                                                                   >  2:112447/1‑135 (MQ=255)
                                                               gTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGt                                                            <  8:254219/136‑1 (MQ=255)
                                                                tGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGt                                                            <  4:102611/135‑1 (MQ=255)
                                                                tGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGt                                                            <  1:4332/135‑1 (MQ=255)
                                                                tGCTCAGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGt                                                            <  2:235455/135‑1 (MQ=255)
                                                                 gCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGCAGCCGAAGTCGTCCTCAGAGCCGAGGGAACCCAGCACCCACGGCCCGGCCAACTGGCTCCGGGCGTa                                                           >  5:215205/1‑135 (MQ=255)
                                                                  cgcGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAg                                                          <  6:4468/133‑1 (MQ=255)
                                                                  cTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAg                                                          <  3:68836/135‑1 (MQ=255)
                                                                   tCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAg                                                          <  6:182633/134‑1 (MQ=255)
                                                                      gcgcTCCACCACGCCCCAGCAGAACCCGCGGCGCGGCCAGCAGCCGGCCGGTCGCCGCCCAGAAGCCGCAGTCGTCATCAGAGCCGAGGCCACCCAGCCCCAACGGCCCGGCCAACTGGCTCCGGGCGTAgctgc                                                      >  4:41237/1‑135 (MQ=255)
                                                                      gcgcTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGaa                                                                                                                       >  4:213350/1‑70 (MQ=255)
                                                                      gcgcTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGaa                                                                                                                       <  3:213350/70‑1 (MQ=255)
                                                                       cgcTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGaa                                                                                                  <  5:247047/90‑1 (MQ=255)
                                                                       cgcTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGaa                                                                                                  >  6:247047/1‑90 (MQ=255)
                                                                       cgcTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGctgctg                                                    <  7:180281/136‑1 (MQ=255)
                                                                       cgcTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGctgctg                                                    <  7:74945/136‑1 (MQ=255)
                                                                            caccacGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCAt                                                <  1:62264/135‑1 (MQ=255)
                                                                                acGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAgc                                           <  8:237604/136‑1 (MQ=255)
                                                                                 cGCCCCAGCAGAACCAGCGGCGCGGCCTGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGATGAAACCCAGCACCAACTGCCCGGCCATCTGGCTCAGGGCGTAGCTGCTGCCATACAgc                                           <  3:222162/135‑1 (MQ=255)
                                                                                  gCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAgcg                                          >  4:122961/1‑135 (MQ=255)
                                                                                    cccAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGcc                                        >  4:8053/1‑135 (MQ=255)
                                                                                    cccAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGcc                                        >  1:79535/1‑135 (MQ=255)
                                                                                                     cgcggcCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCCCCACCCGgccccgcca                       >  2:186285/1‑135 (MQ=255)
                                                                                                         gcCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCCACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGt                   >  3:73931/1‑135 (MQ=255)
                                                                                                           cagcagcagGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCg                <  8:277350/136‑1 (MQ=255)
                                                                                                                       ggTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAgttg     >  1:107127/1‑135 (MQ=255)
                                                                                                                         tggccgccCTCATGCCGATGTCGCCATCAGAGCGGTGTAAACCCCGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAgttggtt  <  7:236463/134‑1 (MQ=255)
                                                                                                                                    |                                                                                                                            
GCAATTACCGGCAAGCGTCGGCAGAAGGCGAACAGGTCGCCAAAGGTGACACTGCAGGCTTCCGTGCTCGGCGCTCCACCACGCCCCAGCAGAACCAGCGGCGCGGCCAGCAGCAGGCCGGTCGCCGCCCAGAAGCCGAAGTCGTCATCAGAGCCGAGGAAACCCAGCACCAACGGCCCGGCCAACTGGCTCAGGGCGTAGCTGCTGCCATACAGCGCCACCAACCGGCCACGCCACTGTTCGACCACCAGTTGGTT  >  KT2440/6021084‑6021340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑