Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	6,174,824	T→G	31.5%	N153T (AAC→ACC)	atpB ←	ATP synthase subunit a

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	6,174,824	0	T	G	31.5%	39.9 / 2.8	38	N153T (AAC→ACC)	atpB	ATP synthase subunit a
Reads supporting (aligned to +/- strand):  ref base T (6/19);  new base G (11/2);  total (17/21)
Fisher's exact test for biased strand distribution p-value = 5.42e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCACGAAGATATTCTTGCTGCCAAACGGGTGCAGGGTCAGCTCACCGATGAAGCCGCCCAGTCCCTTGACCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCGGGTCGGTGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAAATCAGGATGGCCAGCTGAGGAATCCAGTCGACCGGTATCAGGTCGACGGCGTTCATCAGGAATACCCAGACGAAAATGGT  >  KT2440/6174694‑6174954                                                                                                                                   |                                                                                                                                   cTGCACGAAGATATTCTTGCTGCCAAACGGGTGCAGGGTCAGCTCACCGATGAAGCCGCCCAGTCCCTTGACCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCgg                                                                                                                                >  2:62030/1‑135 (MQ=255)    cACGAAGATATTCTTGCTGCCAAACGGGTGCAGGGTCAGCTCACCGATGAAGCCGCCCAGTCCCTTGACCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCg                                                                                                                                 <  7:746/131‑1 (MQ=255)    cACGAAGATATTCTTGCTGCCAAACGGGGGCAGGGTCAGCTCACCGATGAAGCCGCCCAGTCCCTTGACCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCg                                                                                                                                 <  7:163740/131‑1 (MQ=255)      cGAAGATATTCTTGCTGCCAAACGGGTGCAGGGTCAGCTCACCGATGAAGCCGCCCAGTCCCTTGACCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCGGGTCggt                                                                                                                          >  7:146577/1‑136 (MQ=255)      cGAAGATATTCTTGCTGCCAAACGGGTGCAGGGTCAGCTCACCGATGAAGCCGCCCAGTCCCTTGACCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGGTGGGGTCggg                                                                                                                          >  8:128531/1‑135 (MQ=255)      cGAAGATATTCTTGCTGCCAAACGGGTGCAGGGTCAGCTCACCGATGAAGCCGCCCAGTCCCTTGACCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGGTGGGGGCgg                                                                                                                           >  5:12825/1‑135 (MQ=255)       gAAGATATTCTTGCTGCCAAACGGGTGCAGGGTCAGCTCACCGCTGAAGCCGCCCAGTCCCTTGACCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCGGGTCggt                                                                                                                          <  5:43737/135‑1 (MQ=255)           atatTCTTGCTGCCAAACCGGGGCAGGGTCAGCTCACCTATGAAGCCGCCCAGTCCCTTGACCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGGCGCGTGCGGGTCGGTGGTCg                                                                                                                     <  7:212122/136‑1 (MQ=255)             atactggctgcCAAACGGGGGCAGGGGCAGCGCACCGATGAAGCCGCCCAGTCCCTTGACCTAGATGCTGTAGAAGAAGAGCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCGGGTCGGTGGGCg                                                                                                                     <  1:214122/128‑1 (MQ=255)                               gTGCAGGGTCAGCTCACCGATGAAGCCGCCCCGTCCCTTTACCTGGATGCTGTAGAAGCTGAGCAGGGCGAAGCCGCAGAAGGCCATGGCCAGGGTCGCGGTCGGGGCGGTGGTCGACACGGCGCGGAACGGAAt                                                                                                  <  1:74120/135‑1 (MQ=255)                                  cAGGGTCAGCTCACCGATGAAGCCGCCCAGTCCCTTGCCCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTAGGGGCGGGGGTCGGGACTGCGCGGGCCGGAATGTGc                                                                                              >  7:71777/1‑136 (MQ=255)                                              aCCGATGAAGCCGCCCAGTCCCTTGACCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCGGGTCGGTGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACCgg                                                                                    <  8:21923/134‑1 (MQ=255)                                                       gccgcaCAGTCCCTTGACGTTGATGCTGTAGAAGATGAGCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGGTCGGGGCGGTGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAAATCAGGAt                                                                          <  4:161137/135‑1 (MQ=255)                                                        ccgccCAGTCCCTTGACCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGGTGGGGTCGGTGGGCGACACCGCGCGGGACGGAATGTGCGGGTGACCCGAAATCAGGATgg                                                                        >  7:262726/1‑136 (MQ=255)                                                        ccgccCAGTCCCTTGACCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGGTCGGGGCGGGGGTCGCCACGGCGCGGCACGGAATGTGCGGGTCCCCGGAAATCAGGATgg                                                                        >  8:90173/1‑136 (MQ=255)                                                            ccAGTCCCTTGACCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCGGGTCGGTGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAAATCAGGATGGCCAg                                                                    <  7:128531/136‑1 (MQ=255)                                                              aGTCCCTTGACCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGAGGTAGGGTCGGTTGTCGACACGGCACGGAACGGAATGTTCGTGTCCCCGGAAATCATGATGGCCaaa                                                                   >  6:125786/1‑133 (MQ=255)                                                                        ccttgaTGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGGTGGGGTGGGTGGTCGACACCGCGCGGCACCGAATGTTCGGGTGACCGGAAATCACGATGGCCACCAGAGGAATcc                                                         >  8:52940/1‑135 (MQ=255)                                                                            gaTGCTGTAGACGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCGGGGCGGGGGGCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAAATCAGGATGGCCAGCTGAGGAATCCAGTc                                                     <  7:52940/135‑1 (MQ=255)                                                                            gaTGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCGGGTCGGTGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAAATCAGGATGGCCAGCTGAGGAATCCAGTc                                                     <  7:233861/135‑1 (MQ=255)                                                                             aTGCTGTAGAAGATGATCAGGGCGAAGACGCAGACGGCCATGGCCAGGGTCGCGTTCGGGTCGGTGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAAATCAGGATGGCCAGCTGAGGAATCCAGTc                                                     <  4:230390/134‑1 (MQ=255)                                                                             aTGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCGGGTCGGTGGGCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAAATCAGGATGGCCAGCTGAGGAATCCAGTc                                                     <  1:62030/134‑1 (MQ=255)                                                                             aTGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCGGGGCGGTGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAAATCAGGATGGCCAGCTGAGGAATCCAGTc                                                     <  5:222380/134‑1 (MQ=255)                                                                               gCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCGGGTCGGTGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAAATCAGGATGGGCCGCTGAGGCATCCAGTCGAcc                                                 >  8:233861/1‑136 (MQ=255)                                                                               gCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGGTGGGGGCGGTGGTGGACACGGCGCGGAACGGAATTTTCGGGTCACCGGAAATCAGGAAGGCCAGCTGCGGCATCCCGTGGAc                                                  >  6:222380/1‑135 (MQ=255)                                                                                              cAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGGTAGGGTCGGTGGTGGCCACGGCGCGGCACGGGATGTGGGGGTCACCCGTAATACGGCTTGCCCGCTGTGGTATACACTCGCCCGGGATGAggggggc                                   >  3:266164/1‑130 (MQ=255)                                                                                                  gCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCGGGTCGGTGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAAATCAGGATGGCCAGCTGAGGAATCCAGTCGACCGGTATCAGGTCGACGGCGt                              >  7:205342/1‑136 (MQ=255)                                                                                                   cgaccaCGCAGAAGGCCATGGCCAGGGTCGCGTGCGGGTCGGTGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAAATCAGGATGGCCAGCTGAGGAATCCAGTCGACCGGTATCAGGTCGACGGCGtt                             <  7:220200/131‑1 (MQ=255)                                                                                                    gacgccGCAGAAGGCCATGGCCAGGGTCGCGTTCGGGTCGGGGGGCGACACGGCGCGGAACGGAATGTGCGGGCCACCGGAAATCAGGATGGCCAGCTGAGGAATCCAGTCGACCGGTATCAGGTCGACGGCGtt                             <  4:69870/130‑1 (MQ=255)                                                                                                    gAAGCCGCAGACGGCCATGGCCAGGGGCGCGTTCGGGGCGGGGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAAATCAGGATGGCCATCTGAGGAATCCAGTCGACCGGTATCAGGTCGACGGCGtt                             <  5:125786/135‑1 (MQ=255)                                                                                                        aCGCCGAAGGCCCTGGCCTGGGTCGCTTTCGGGTCGGGGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAATTCAGGATGGCCATCTGAGGAATCCAGTCGACCGGTATCCGGTCGACGGCGTtcatca                        <  8:146577/136‑1 (MQ=255)                                                                                                              aaGGCCATGGCCAGGGTCGCGGTCGGGTCGGTGGTCGCCACGGCGCGGCACGGAATGTGCGGGGCACCGGAAATCAGGATGGCCAGCTGAGGAATCCAGTCGACCGGGATCAGGTCGACGGCGGTGATCAGGAAt                   >  3:256338/1‑135 (MQ=255)                                                                                                              aaGGCCATGGCCAGGGTCGCGGTCGGGTCGGTGGTCGACACGGCGCGGCACGGAATGTGCGGGTCACCGGAAATCAGGATGGCCAGCTGAGGAATCCAGTCGCCCGGGATCAGGTCGACGGCGGTCATCAGGAAt                   >  4:111205/1‑135 (MQ=255)                                                                                                                 gCCATGGCCAGGGTCGCGTTCGGGTCGGGGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAAATCAGGATGGCCAGCTGAGGAATCCAGTCGACCGGTATCAGGTCGACGGCGTTCATCAGGAATAcac               <  7:90173/136‑3 (MQ=255)                                                                                                                       gCCAGGGTCGCGGTCGGGTCGGTGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACAGGAAATCAGGATTGGCAGCTGACGAATACAGTCGAACGGTATCAGGTCGACGGCGGTCATCAGGAATACCCacac           >  6:83192/1‑134 (MQ=255)                                                                                                                         cAGGGTCGCGTTAGGGTCGGTGGTCGACACGGGGCGGAACGGAAAGTGCGGGTGACCGGAAATCAGCATGGCCACCTGAGGAATCCAGTAGCCCCGTTTCAGGGCGGCGGCGTTGATCAGGAATAACCagaaga         >  4:126668/1‑134 (MQ=255)                                                                                                                             gTCGCTTTGGGGGGGGTGGTCGACCGGGCGGTTAAAGTAATGGGCGGGGCACCGTTAATACTGATGCCCAGCTGTGGAATCCAGTCGACCGGTATCAGGGCGAGGGCGTTCATCAGGAATACCCAGACGAAAATg    <  2:63308/135‑1 (MQ=255)                                                                                                                              tCGCGTTCGGGTCGGTGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAAATCAGGATGGCCAGCTGAGGAATCCAGTCGACCGGTATCAGGTCGACGGCGTTCATCAGGAATACCCAGACGAAAATGGt  <  8:205342/136‑1 (MQ=255)                                                                                                                                   |                                                                                                                                   CTGCACGAAGATATTCTTGCTGCCAAACGGGTGCAGGGTCAGCTCACCGATGAAGCCGCCCAGTCCCTTGACCTTGATGCTGTAGAAGATGATCAGGGCGAAGACGCAGAAGGCCATGGCCAGGGTCGCGTTCGGGTCGGTGGTCGACACGGCGCGGAACGGAATGTGCGGGTCACCGGAAATCAGGATGGCCAGCTGAGGAATCCAGTCGACCGGTATCAGGTCGACGGCGTTCATCAGGAATACCCAGACGAAAATGGT  >  KT2440/6174694‑6174954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑