Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 345,224 C→T 6.4% A220A (GCC→GCT)  hisA → 1‑(5‑phosphoribosyl)‑5‑[(5‑phosphoribosylamino)m ethylideneamino] imidazole‑4‑carboxamide isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440345,2240CT 6.4% 202.7 / 2.6 62A220A (GCC→GCT) hisA1‑(5‑phosphoribosyl)‑5‑[(5‑phosphoribosylamino)m ethylideneamino] imidazole‑4‑carboxamide isomerase
Reads supporting (aligned to +/- strand):  ref base C (21/37);  new base T (2/2);  total (23/39)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGGCTGCAACGTGCCCTTCACCAAGGCACTGGCTGAAGCCACCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGCCAAGCGCATTATCCCTTGCCTG  >  KT2440/345091‑345345
                                                                                                                                     |                                                                                                                         
aGGGCTGCAACGTGCCCTTCACCAAGGCACTGGCTGAAGCCACCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCa                                                                                                                          >  5:238271/1‑135 (MQ=255)
     tGCAACGTGCCCTTCACCAAGGCACTGGCTGAAGCCACCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACTCCAAGGCCCCGGGCAGAATCGGCGCCATCAcc                                                                                                                     >  2:224832/1‑135 (MQ=255)
     tGCAACGTGCCCTTCACCAAGGCACTGGCTGAAGCCACCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCAcc                                                                                                                     >  2:39938/1‑135 (MQ=255)
          cGTGCCCTTCACCAAGGCACTGGCTGAAGCCACCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCg                                                                                                                >  6:72710/1‑135 (MQ=255)
          cGTGCCCTTCACCAAGGCACTGGCTGAAGCAACACGTATCCCGGTGATCGCCTCTGGCGGCATTCACAACCTGGGTGACATCAAGTACCTGATGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGTcct                                                                                                                >  5:32071/1‑134 (MQ=255)
           gTGCCCTTCACCAAGGCACTGGCTGAAGCCACCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGt                                                                                                               <  1:39938/135‑1 (MQ=255)
               ccTTCACCAAGGCACTGGCTGAAGCCACCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGcc                                                                                                            <  1:77451/134‑1 (MQ=255)
                     ccAAGGCACTGGCTGAAGCCACCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACg                                                                                                     <  1:125549/135‑1 (MQ=255)
                     ccAAGGCACTGGCTGAAGCCACCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACg                                                                                                     >  7:89773/1‑135 (MQ=255)
                     ccAAGGCACTGGCTGAAGCCACCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGa                                                                                                    <  8:59521/136‑1 (MQ=255)
                         ggCACTGGCTGAAGCCACCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAgg                                                                                                 >  3:108315/1‑135 (MQ=255)
                            aCTGGCTGAAGCCACACGTATCCCGGTGATCGCCTCGGGAGGCAGTCACAACCTGGGTGACATCAAGGCCCTGCTTGACGCAAAGGCCCAGGGCATCATCGGCGCCATCACCGGCCGTGCCAAATAAGAAGGCAc                                                                                              >  1:121930/1‑135 (MQ=255)
                                   gAAGCCACCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCCTCTACGCAGGCCCCCGGGAt                                                                                       >  2:140212/1‑135 (MQ=255)
                                      gCCACCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTc                                                                                    <  4:77320/135‑1 (MQ=255)
                                      gCCACCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCg                                                                                   <  7:205366/136‑1 (MQ=255)
                                       cctcccGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCAGCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCg                                                                                   <  6:250340/132‑1 (MQ=255)
                                         aCCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGc                                                                                  >  4:198913/1‑134 (MQ=255)
                                            cGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAg                                                                              <  2:8172/135‑1 (MQ=255)
                                            cGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAg                                                                              <  2:33393/135‑1 (MQ=255)
                                                tCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGccc                                                                          <  8:138347/135‑1 (MQ=255)
                                                tCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGccc                                                                          <  7:213579/135‑1 (MQ=255)
                                                 cccGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACAGCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGccc                                                                          <  6:8579/134‑1 (MQ=255)
                                                      tGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCt                                                                    <  4:166235/135‑1 (MQ=255)
                                                       gATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTc                                                                  <  7:108547/136‑1 (MQ=255)
                                                        aTCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCTATCACCGGCCGTGCCATCTACGACGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCt                                                                 >  8:127899/1‑136 (MQ=255)
                                                        aTCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCTATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCt                                                                 >  8:131832/1‑136 (MQ=255)
                                                            ccTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCtgtg                                                              <  5:109301/135‑1 (MQ=255)
                                                            ccTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCtgtg                                                              <  3:198913/135‑1 (MQ=255)
                                                            ccTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCtgtg                                                              <  1:58861/135‑1 (MQ=255)
                                                                   cggcATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTa                                                       <  1:54096/135‑1 (MQ=255)
                                                                       aTTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAg                                                  <  7:273920/136‑1 (MQ=255)
                                                                        ttCACATCCTGGGTGACATCTAGGCCCTGCTGGACGCCAAGGCCCCGGGCTTCTTCTGCGCCATCTCCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAg                                                  <  4:164546/135‑1 (MQ=255)
                                                                        ttCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAg                                                  <  1:140212/135‑1 (MQ=255)
                                                                        ttCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAg                                                  <  3:26267/135‑1 (MQ=255)
                                                                        ttCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAg                                                  <  1:1711/135‑1 (MQ=255)
                                                                             aaCCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCGAGGCCTTCTGTGACAACTACCAAGGCTGa                                             <  6:36658/135‑1 (MQ=255)
                                                                               ccTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGa                                          <  8:69229/136‑1 (MQ=255)
                                                                                      gACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCATGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAAc                                    >  8:26926/1‑135 (MQ=255)
                                                                                         aTCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATg                                <  8:247714/136‑1 (MQ=255)
                                                                                         aTCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCATGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATg                                <  7:26926/136‑1 (MQ=255)
                                                                                            aaGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCa                             >  7:92184/1‑136 (MQ=255)
                                                                                                 ccTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCTATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGc                        <  7:131832/136‑1 (MQ=255)
                                                                                                 ccTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCTATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGc                        <  7:127899/136‑1 (MQ=255)
                                                                                                 ccTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGc                        <  7:19441/136‑1 (MQ=255)
                                                                                                  ctgctgGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGc                        <  4:226046/135‑1 (MQ=255)
                                                                                                   tgctgGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGcc                       <  3:165024/135‑1 (MQ=255)
                                                                                                      tgGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTa                                                       >  7:60692/1‑100 (MQ=255)
                                                                                                      tgGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTa                                                       <  8:60692/100‑1 (MQ=255)
                                                                                                      tgGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGa                                          >  8:211643/1‑113 (MQ=255)
                                                                                                      tgGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGa                                          <  7:211643/113‑1 (MQ=255)
                                                                                                      tgGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGc                        <  4:93998/131‑1 (MQ=255)
                                                                                                      tgGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGc                        >  3:93998/1‑131 (MQ=255)
                                                                                                      tgGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACCCAGGCACCCTGGATGTCGCTGAGGCCCACGCCTGCTGTGACAACTACCAAGGCTGCGGACTGAACCATGGCACTGGCCAAgc                   >  8:19441/1‑136 (MQ=255)
                                                                                                             cAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCCTCTACGGAGGCCCCCTGGATGTCGCTGAGGCCCCCGCCGTAGGTGGCAACAACCCAGGCGGAGGGCCGCACCCTGGCCCTGGCCAAGCGCCTTa             >  2:127174/1‑135 (MQ=255)
                                                                                                             cAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGc                        >  6:152337/1‑124 (MQ=255)
                                                                                                             cAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGc                        <  5:152337/124‑1 (MQ=255)
                                                                                                                ggCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCCCCCTGGATGTCGCTGAGGCCCCGGCCTTCTGTGGCAACTACCAAGGCTGAGGCCTGAACCATGGCACTGGCCCAGCGCATTATccc         >  7:52998/1‑136 (MQ=255)
                                                                                                                ggCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGc                        <  6:275942/121‑1 (MQ=255)
                                                                                                                ggCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGc                        >  5:275942/1‑121 (MQ=255)
                                                                                                                   cccGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGCCAAGCGCATTATCCCtt       <  3:108484/135‑1 (MQ=255)
                                                                                                                       ggCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGCCAAGCGCATTATCCCTTGCCTg  <  7:132570/136‑1 (MQ=255)
                                                                                                                        gCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGCCAAGCGCATTATCACTTGCCTg  >  1:145805/1‑135 (MQ=255)
                                                                                                                                    ccATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGc                        <  7:73990/101‑1 (MQ=255)
                                                                                                                                    ccATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGc                        >  8:73990/1‑101 (MQ=255)
                                                                                                                                     |                                                                                                                         
AGGGCTGCAACGTGCCCTTCACCAAGGCACTGGCTGAAGCCACCCGTATCCCGGTGATCGCCTCGGGCGGCATTCACAACCTGGGTGACATCAAGGCCCTGCTGGACGCCAAGGCCCCGGGCATCATCGGCGCCATCACCGGCCGTGCCATCTACGAAGGCACCCTGGATGTCGCTGAGGCCCAGGCCTTCTGTGACAACTACCAAGGCTGAGGACTGAACCATGGCACTGGCCAAGCGCATTATCCCTTGCCTG  >  KT2440/345091‑345345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑