Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 359,946 T→A 8.6% E140V (GAG→GTG)  CJ019_00333 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440359,9460TA 8.6% 136.8 / 2.5 46E140V (GAG→GTG) CJ019_00333hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/20);  new base A (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

CGGGGCGAACCCCGCTGCATTGAAGCGTGCACCGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGG  >  KT2440/359816‑360077
                                                                                                                                  |                                                                                                                                   
cGGGGCGAACCCCGCTGCATTGAAGCGTGCACCGAGGTGAGCTAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATg                                                                                                                                 <  3:206351/135‑1 (MQ=255)
   ggCGAACCCCGCTGCATTGAAGCGTGCACCGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCACATGGTc                                                                                                                              >  7:123221/1‑135 (MQ=255)
   ggCGAACCCCGCTGCATTGAAGCGTGCACCGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCACATGGTc                                                                                                                              >  7:126607/1‑135 (MQ=255)
    gCGAACCCCGCTGCATTGACGCGTGCACCGAGGGGATCTAATATCTGGTTCAACTTGCTGGTGGGCTCGACAGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCa                                                                                                                            <  7:109634/136‑1 (MQ=255)
      gAACCCCGCTGCATTGAAGCGTGCACCGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGACCAtt                                                                                                                          <  7:32929/136‑1 (MQ=255)
            cGCTGCATTGAAGCGTGCACCGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGAt                                                                                                                     <  1:20761/135‑1 (MQ=255)
              agccATTGAAGCGTGGTCCGAGGTGTGCTAATATCAGGTTCAACTTTCTGGTGGGCTCGTCGGGGTCGGTAATGCAGAAGGCGTAAAACAGGATCGCAACCGGGACGTGGTCCCCCTCATGGTCCATTTCGATACt                                                                                                                  <  8:170781/133‑1 (MQ=255)
               tGCATTGTAGAGTGCACCGTGTTGATCGAATATCAGTTTCAACTTTCTGTTGGGCTAGACCGGGTCGGTATTGGAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACt                                                                                                                  <  4:161384/135‑1 (MQ=255)
               tGCATTGAAGCGTGCACCGAGGTGATCTAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACt                                                                                                                  <  5:94701/135‑1 (MQ=255)
                      aaGCGTGCACCGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCCACCGGGAGCTGCTCCCCCTCATGGTCGATTTCGATACTGAGAtg                                                                                                            >  6:255729/1‑134 (MQ=255)
                        gCGTGCACCGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGaa                                                                                                        <  7:195121/136‑1 (MQ=255)
                        gCGTGCACCGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGaa                                                                                                        <  7:131709/136‑1 (MQ=255)
                         cGTGCACCGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGAtgtg                                                                                                          >  7:82229/1‑133 (MQ=255)
                              aCCGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCGATTTCGATACTGAGATGAGAAGAGGct                                                                                                  >  7:170781/1‑135 (MQ=255)
                              aCCGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGc                                                                                                   >  4:218569/1‑135 (MQ=255)
                              aCCGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGc                                                                                                   >  1:128297/1‑135 (MQ=255)
                              aCCGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGc                                                                                                   >  1:9281/1‑135 (MQ=255)
                              aCCGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCg                                                                                                  >  7:251260/1‑136 (MQ=255)
                                cGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGt                                                                                                 >  5:134630/1‑135 (MQ=255)
                                           aaTATCAGGTTCAACTTGCTGGTGGGCGCGACCGGGGCGGTAATGCAGTAGTCTTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTg                                                                                      <  4:180220/135‑1 (MQ=255)
                                            atatCAGGTTCAACTTTCGGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTg                                                                                      <  7:103507/134‑1 (MQ=255)
                                                 aGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCAGGGTCCATTTCGATACTGAGATGTGAAGCGGCGTGCATGACGGTGAAgg                                                                                  >  5:42994/1‑133 (MQ=255)
                                                         cTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTAcc                                                                        >  4:146250/1‑135 (MQ=255)
                                                          tttCGGGTGGGCTCGACCGGTTCGGTAATGCCGAAGCCGTAAAACAGGAGCCCACCCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCt                                                                       <  2:267257/135‑1 (MQ=255)
                                                                        gACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGTAGTTCATGACGGTGCAGGGACGCGTACCTGCCGACTGCAgcgc                                                         >  3:177194/1‑135 (MQ=255)
                                                                          cggggTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCACATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGc                                                      <  8:123221/134‑1 (MQ=255)
                                                                          ccGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCACATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGc                                                      <  8:126607/136‑1 (MQ=255)
                                                                                cGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGa                                                  >  4:157263/1‑134 (MQ=255)
                                                                                        ccgTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTtcgg                                        <  8:129090/134‑2 (MQ=255)
                                                                                         aGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTtcgt                                        <  2:150581/135‑1 (MQ=255)
                                                                                           tAGGCGTAAACCAGTTTCGAAACCGGGACCTGGTCCCCCTGATGGTCCATTTCGATACTGAGATGTGATGTGGCGTTCATGACGGTGAAGGTACGCGTACCTGCGGACTGCAGAGCGGCTTGAACATTTtcgtcg                                      <  2:146859/135‑1 (MQ=255)
                                                                                           tAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTtcgt                                        >  1:173690/1‑133 (MQ=255)
                                                                                              gCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGCGCGGCTTGAACATTTTCGTCGAGGc                                  >  7:173711/1‑136 (MQ=255)
                                                                                                     aCAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCGCGTGag                           >  7:104392/1‑135 (MQ=255)
                                                                                                      cAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACt                                                                                                                  >  8:4172/1‑48 (MQ=255)
                                                                                                      cAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACt                                                                                                                  <  7:4172/48‑1 (MQ=255)
                                                                                                      cAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGaa                           >  2:57634/1‑135 (MQ=255)
                                                                                                         gATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCa                        <  2:128297/135‑1 (MQ=255)
                                                                                                            cGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACt                                                                                                                  <  8:69595/42‑1 (MQ=255)
                                                                                                            cGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACt                                                                                                                  >  7:69595/1‑42 (MQ=255)
                                                                                                             gCACCCGGGACCTGGTCCCCCGCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGcc                   <  8:1947/136‑1 (MQ=255)
                                                                                                                      aCCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTcg           >  6:110298/1‑135 (MQ=255)
                                                                                                                       ccTGGACCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACAGTTTCGTAGCGGCTCTTGAACCAAGGCCCTTGTTctc          >  6:274371/1‑133 (MQ=255)
                                                                                                                           ggcccacTCATGGTCCCTTTCGATCCTGAGATGGGCCGCGGCGGTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAgg      <  2:239017/133‑1 (MQ=255)
                                                                                                                              ccccTGATGGTCCATTTCGATACTGAGATGTGAAGAGGCGGTCATGACGGTGAAGGTACGCGTTCCTGCCGACTGCAGAGCGGCTTGAAGATTTTCGTGGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCgg   <  3:68827/135‑1 (MQ=255)
                                                                                                                              ccccTCTTGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCAGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCACGGCCCGTTTTAGAGAGGCggg  >  8:57217/1‑136 (MQ=255)
                                                                                                                                  |                                                                                                                                   
CGGGGCGAACCCCGCTGCATTGAAGCGTGCACCGAGGTGATCGAATATCAGGTTCAACTTTCTGGTGGGCTCGACCGGGTCGGTAATGCAGTAGGCGTAAAACAGGATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGG  >  KT2440/359816‑360077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑