Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 4,914,804 G→A E474K (GAG→AAG)  CJ019_04610 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,914,8040GA97.5% 135.5 / ‑3.9 40E474K (GAG→AAG) CJ019_04610hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (23/16);  total (24/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01

GCCAAACAGGAGGGGGTGGGCGACATCGCCTGGGGGCTGTTCAACGCCGTACCGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCGAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGGCGCGGAGTACTTCCACCACCACGAGCCGATTACACCGCTTGACGAAGGCGACCCGGCTGTTGCCG  >  KT2440/4914678‑4914936
                                                                                                                              |                                                                                                                                    
gCCAACCCGGAGGGGGGGGGCGACATCGCCGGGGGGCTGTTCAACGCCGTACCGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAAc                                                                                                                              <  1:156182/135‑1 (MQ=255)
gCCAAACAGGAGGGGGTGGGCGACATCGCCTGGGGGCTGTTCAACGCCGTACCGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAAc                                                                                                                              <  8:157811/135‑1 (MQ=255)
gCCAAACAGGAGGGGGTCGGCGACATCGCCTGCGGGCTGTTCAACGCCGTACCGCTGGAAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACAGTTTTGTCATGCCGCGCAAGGTCAAc                                                                                                                              >  1:41763/1‑135 (MQ=255)
      cAGGAGGGGGTGGGCGACATCGCCTGGGGGCTGTTCAACGCCGTACCGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAg                                                                                                                        >  1:140119/1‑135 (MQ=255)
           ggggggggGCGCCATCGCCGGGGGGCTGTTCAACGCCGTACCGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCgg                                                                                                                   <  1:275540/135‑1 (MQ=255)
                    cGACCTCGCCTGGGGGCTGTTCAACGCCGTACCGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCgg                                                                                                                   <  2:201149/126‑1 (MQ=255)
                    cGACATCGCCTGGGGGCTGTTCAACGCCGTACCGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCgg                                                                                                                   >  1:201149/1‑126 (MQ=255)
                    cGACATCGCCTGGGGGCTGTTCAACGCCGTACCGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGc                                                                                                           >  6:151289/1‑134 (MQ=255)
                    cGACATCGCCTGGGGGCTGTTCAACGCCGTACCGCTAGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGc                                                                                                           >  3:180990/1‑134 (MQ=255)
                          cGCCTGGGGGCGGTTCAACGCCGTACCGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGccc                                                                                                    <  5:244017/135‑1 (MQ=255)
                          cGCCGGGGGGCTGTTCAACGCCGTACCGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGccc                                                                                                    <  4:264804/135‑1 (MQ=255)
                           gCCTGGGGGCTGTTCACCGCCGTACCGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCg                                                                                                   >  7:181680/1‑135 (MQ=255)
                            ccTGGGGGCTGTTCAACGCCGTACCGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGccc                                                                                                    <  2:209505/133‑1 (MQ=255)
                                        tCAACGCCGTACCGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTATGCCCGACCAGTCcaccac                                                                                      >  2:262772/1‑135 (MQ=255)
                                                    cGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCGAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGc                                                                          >  3:22015/1‑135 (MQ=255)
                                                    cGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCACCGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCCCGGCTTCCCGAGc                                                                          >  1:93079/1‑135 (MQ=255)
                                                    cGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAg                                                                           >  7:35006/1‑134 (MQ=255)
                                                          cAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGCCGGTTGTGTCATGCCGCGCAAGGGCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAg                                                                    <  6:240630/135‑1 (MQ=255)
                                                           aaCGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAgg                                                                   >  6:108751/1‑135 (MQ=255)
                                                              ggAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGgcgc                                                                <  8:148864/135‑1 (MQ=255)
                                                                  ggCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGGCGCGGAg                                                            <  6:169977/135‑1 (MQ=255)
                                                                                 aaGGCCCTCTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGCGCCAGAAGGCGCGGAGTACTTccaccacca                                              >  7:103902/1‑134 (MQ=255)
                                                                                  aGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGGCGCGGAGTACTTCCACCACCACg                                            <  2:219090/135‑1 (MQ=255)
                                                                                    gCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGCGCCAGAAGGCGCGGAGTACTTCCACCACCACGAg                                          >  2:271927/1‑135 (MQ=255)
                                                                                    gCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGGCGCGGAGTACTTCCACCACCACGAg                                          >  1:10507/1‑135 (MQ=255)
                                                                                         gTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGGCGCGGAGTACTTCCACCACCACGCGCCGa                                      >  1:267825/1‑134 (MQ=255)
                                                                                         gTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGGCGCGGAGTACTTCCACCACCACGAGCCGa                                      >  3:164817/1‑134 (MQ=255)
                                                                                           tCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCGACCACGGCTTCCCGAGCCAGATGGCGCGGAGTACTTCCACCACCACGAGCCGAtt                                    <  3:253215/134‑1 (MQ=255)
                                                                                           tCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGGCGCGGAGTACTTCCACCACCACGAGCCGAtt                                    >  4:100442/1‑134 (MQ=255)
                                                                                           tCGAGTTCAAAAGCGACGGTTTTGTCATGCCGAGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGGCGCGGAGTACTTCCACCACCACGAGCCGAtt                                    <  8:146299/134‑1 (MQ=255)
                                                                                             gAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGGCGCGGAGTACTTCCACCACCACGAGCCGATTaca                                 >  5:13032/1‑135 (MQ=255)
                                                                                                 tCAAAAGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGATAAGCACGACCAGTCCACCACGGCTTCCCGAGCCAGAAGGCGCGGAGTACTTCCACCACCACGAGCCGAGTACAcccc                             >  3:172152/1‑133 (MQ=255)
                                                                                                     aaGCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGCCACGGAGTACTTCCACCACCACGAGCCGATTACACCGCGTGa                         >  2:134241/1‑135 (MQ=255)
                                                                                                       gCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGccc                                                                                                    <  5:20288/58‑1 (MQ=255)
                                                                                                       gCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGccc                                                                                                    >  4:266019/1‑58 (MQ=255)
                                                                                                       gCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGccc                                                                                                    >  6:20288/1‑58 (MQ=255)
                                                                                                       gCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGccc                                                                                                    <  3:266019/58‑1 (MQ=255)
                                                                                                       gCGACGGTTTTGTCATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGGCGCGGAGTACTTCCACCACCACGAGCCGATTACACCGCTTGAc                        >  8:52543/1‑134 (MQ=255)
                                                                                                                    cATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGccc                                                                                                    <  4:265280/45‑1 (MQ=255)
                                                                                                                    cATGCCGCGCAAGGTCAACGGGCAGCTCGGCTATCCGCTAAGccc                                                                                                    >  3:265280/1‑45 (MQ=255)
                                                                                                                           cgcaAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGGCGCGGAGTACTTCCACCCCCACGAGCCGATTACACCGCTTGACGAAGGCGACCCGGCTGTTGcc   >  8:24216/1‑135 (MQ=255)
                                                                                                                           cgcaAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGGCGCGGAGTACTTCCACCACCACGAGCCGATTACACCGCTTGACGAAGGCGACCCGGCTGTTGcc   >  3:20289/1‑135 (MQ=255)
                                                                                                                           cgcaAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGGCGCGGAGTACTTCCACCACCACGAGCCGATTACACCGCTTGACGAAGGCGACCCGGCTGTTGcc   >  7:35420/1‑135 (MQ=255)
                                                                                                                            gcgAGGTCACCGGGCAGCGCGGCTATCCGCTACGCCCGACCAGTCCCCCACGGCCGCCCGACCCAGAAGGCGCGGAGTACTTCCACCACCACGAGCCGATTACACCGCTTGACGAAGGCGACCCGGCTGTTGCCg  <  4:9772/135‑1 (MQ=255)
                                                                                                                              |                                                                                                                                    
GCCAAACAGGAGGGGGTGGGCGACATCGCCTGGGGGCTGTTCAACGCCGTACCGCTGGCAACGGAGGGCACAGCAGGCATCAAGGCCCTGTTCGAGTTCAAAAGCGACGGTTTTGTCATGCCGCGCGAGGTCAACGGGCAGCTCGGCTATCCGCTAAGCCCGACCAGTCCACCACGGCTTCCCGAGCCAGAAGGCGCGGAGTACTTCCACCACCACGAGCCGATTACACCGCTTGACGAAGGCGACCCGGCTGTTGCCG  >  KT2440/4914678‑4914936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑