Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440151,0100CG30.0% 55.5 / 13.6 41D44H (GAC→CAC) CJ019_00157hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (8/20);  new base G (12/0);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 4.51e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGCCAGCAGCAGCATGCCGAGGGAAGCGGCAATCAGCGGTTTGCGCATGGAGCA  >  KT2440/150885‑151145
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gcgcAGGGTATTGAAGGCCGCCTCGAGTTGTACGAATCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGGTTCGCGCACCACTTTGGAGGCGCGGTTGGAGGGTGTTGTGTCGGAGGGGGAGt                                                                                                                               >  8:405486/1‑137 (MQ=255)
    aGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGGTGGAGGGGGTGGGGGGTGCGGGGGAGtcgat                                                                                                                           >  8:360408/1‑137 (MQ=255)
     gggTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACGGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGtcgatc                                                                                                                          <  6:378261/137‑1 (MQ=255)
     gggTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGtcgatc                                                                                                                          <  7:287800/137‑1 (MQ=255)
           tGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCCCTTTGGAGTCGCGGGTCGCGGGGGTGGGGTGTGTGGGGTAGTGGATCGAA‑Cgg                                                                                                                   >  6:140492/1‑137 (MQ=255)
                 ccgccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAg                                                                                                             <  8:85103/137‑1 (MQ=255)
                   gccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGgc                                                                                                           <  6:365095/137‑1 (MQ=255)
                   gccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGgc                                                                                                           >  7:75494/1‑137 (MQ=255)
                   gccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACGTTGGAGTCGAGGATCGAGGTGGGGGTGTCTGAGGTGTAGTAGATGGAA‑CGGGCAAATgc                                                                                                           >  7:383304/1‑137 (MQ=255)
                   gccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTAGCGCCCCAATTTGGAGTAGCGGTTAGTGGTGGTGGTGGGTGAGGTGTATTTGGTCGAA‑CGGGAAAAggg                                                                                                           >  8:157427/1‑136 (MQ=255)
                   gccTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCGTAGCGCACCCCTTTTGAGTCGCGGGTGGAGGTGGTGGGGTGTGTTGGGGAGTGGAATGAACCGGCCAAAgc                                                                                                            >  3:417023/1‑136 (MQ=255)
                     cTCGAGTTGTACGCCGCGGATGTCCCCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTTGGCTTCGCGCACCACTTTGGAGTGGCGGCTGGCTGTGGGGGTGTTTGAGGTTAACTATATAGAA‑AGGCCCAATgccc                                                                                                         >  7:253323/1‑135 (MQ=255)
                     cTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCCCCCCTTTGGAGTCGCGGGTAGCTGTGGTGGGGGGGGAGGGGAAGTCGATAGCA‑CCGCCAAACgccc                                                                                                         >  2:391662/1‑135 (MQ=255)
                     cTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCCCCACTTTGGAGGCGCGGGTGGAGGTGGGGGGGGGTGGGGGGAAGTCGCTCGCA‑ACGCCCAAGgccc                                                                                                         >  8:427148/1‑135 (MQ=255)
                     cTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTTGAGTCGCGGCTCGCGGGGGTGGGGTGTGGGGTGAAGTGGTTAGCA‑CCGCCAAAAgccc                                                                                                         >  3:184275/1‑135 (MQ=255)
                     cTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATAGAGGTGGTGGTGTGGGATGTGAAGTCGATCTAA‑CGGCCAAAGgccc                                                                                                         >  7:139922/1‑135 (MQ=255)
                     cTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCGTAGCGCACCACATTGGATTCGCCGCTAGAGGTGGGGGTTGGTGAGGTGAAGTAGAAAGAA‑CCGGCAAAAgccc                                                                                                         >  5:258381/1‑135 (MQ=255)
                     cTCGAGTTGTACGCCGCGGATGTCACCGTTGCAGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTTTCGCACCACTTTTGAGGAGCGGTTGGAGGGGGTGGTGTGTGTTGTGGAGTAGATCGGA‑CCGGCAAAGgcac                                                                                                         >  5:339561/1‑137 (MQ=255)
                     cTCGAGTTGTACGCCGCGGATGTCAACGTTGCTGGCGACGAAGATTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGCTCCAGGTGGTGGGGTCGTTGGTTAAGTCGATAGCA‑CGGCCAAATgccc                                                                                                         >  6:333099/1‑135 (MQ=255)
                      gcGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGgcacg                                                                                                        <  3:35528/136‑1 (MQ=255)
                                      ggATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACGTTGGAGTCGCGGGTCGCGGGGGGGGTGGGTGGGGTGGAGTAGATCGAA‑CCGCCACACGCCCGCCCCCTGGTGTTTCtg                                                                                        >  5:102851/1‑137 (MQ=255)
                                       gATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCtgg                                                                                       <  4:175741/137‑1 (MQ=255)
                                            caacGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCTGGTGGcc                                                                                  <  8:337364/134‑1 (MQ=255)
                                               cGTTGCGGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCTGGTGGccacc                                                                               <  5:192131/137‑1 (MQ=255)
                                                gTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGTTGGCGGGGGTGGTGTGGGTGGTGGAGTGGATCGAA‑CCGCCCAACGCACGCCCGATGATTTTTCTGGTGGgcaccc                                                                              >  7:356069/1‑136 (MQ=255)
                                                   gCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAggg                                                                           <  5:104660/137‑1 (MQ=255)
                                                       gCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCtg                                                                       <  7:154926/137‑1 (MQ=255)
                                                                         gcgtcgtGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGc                                                     <  1:391662/136‑1 (MQ=255)
                                                                         gcgtcgtGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGGGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGGCTGTGCCTGGGCCAGGTCGGc                                                     <  5:302356/136‑1 (MQ=255)
                                                                               ggggCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGAGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGCcagcag                                               <  5:140492/136‑1 (MQ=255)
                                                                                     gcgcgCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGCCAGCAGCAGCAt                                         <  2:408444/136‑1 (MQ=255)
                                                                                         gccccaaTTTGGAGTCGCGGATAGGGGTGGGTGTGTCTGAGGTGAAGTCGATCGAA‑CTGCCACACGCACGCAAGATGATGTTGGTTGTGGGCACCACGGTCTGTGCATGGGGCAGGGCGGCCCGCAGCCGCATGCCg                                     >  2:28067/4‑137 (MQ=255)
                                                                                          acagacTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGCCAGCAGCAGCATGCCGa                                    >  7:223932/5‑137 (MQ=255)
                                                                                               cTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGCCAGCAGCAGCATGCCGAGGGaa                               <  2:113563/137‑1 (MQ=255)
                                                                                                      ggcgcgGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGCCAGCAGCAGCATGCCGAGGGAAGCGGCaa                        <  7:360408/135‑1 (MQ=255)
                                                                                                      gTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCt                                                                                         <  4:8773/72‑1 (MQ=255)
                                                                                                      gTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCt                                                                                         >  3:8773/1‑72 (MQ=255)
                                                                                                             aTCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCCCGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGCCAGCAGCAGCATGCCGAGGGAAGCGGCAATCAGCgg                 >  5:443379/1‑137 (MQ=255)
                                                                                                                  ggttgtggtGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATTTCGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGCCAGCAGCAGCATGCCTAGGGAAGCGGCAATCAGAGGTTTgc            <  8:106601/137‑1 (MQ=255)
                                                                                                                  ggtggtggtGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGCCAGCAGCAGCATGCCGAGGGAAGCGGCAATCAGCGGTTTgc            <  1:380285/137‑1 (MQ=255)
                                                                                                                            tCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGCCAGCAGCAGCATGCCGAGGGAAGCGACAATCAGCGGTTTGCGCATGGAGCa  <  2:7491/137‑1 (MQ=255)
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GCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTCACCGTTGCTGGCGACGAAGCTTGCAGCGTCGTCGTGGGCTTCGCGCACCACTTTGGAGTCGCGGATCGAGGTGGTGGTGTCTGAGGTGAAGTCGATCGAA‑CGGCCAAAGGCACGCACGATGATGTTGCTGGTGGCCACCAGGGTCTGTGCCTGGGCCAGGTCGGCCAGCAGCAGCATGCCGAGGGAAGCGGCAATCAGCGGTTTGCGCATGGAGCA  >  KT2440/150885‑151145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑