Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,115,4440TG26.0% 88.8 / 10.2 50L242R (CTT→CGT) lptGLipopolysaccharide export system permeaseprotein LptG
Reads supporting (aligned to +/- strand):  ref base T (8/29);  new base G (13/0);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 5.73e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACTGGCTGCTCGCCGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAACGGCGGCCCTGGTGCTGA  >  KT2440/1115309‑1115569
                                                                                                                                       |                                                                                                                             
aCTGGCTGCTCGCCGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCtt                                                                                                                              >  2:217848/1‑137 (MQ=255)
aCTGGCTGCTCGCCGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCtt                                                                                                                              >  7:190408/1‑137 (MQ=255)
 cTGGCTGCTCGCCGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCttt                                                                                                                             <  5:417670/137‑1 (MQ=255)
         tCGCCGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTAcc                                                                                                                     <  7:336965/137‑1 (MQ=255)
             cGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGt                                                                                                                 <  4:322397/137‑1 (MQ=255)
                tgtgCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTtgt                                                                                                              <  7:348941/137‑1 (MQ=255)
                  tgCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTgg                                                                                                            <  3:211718/137‑1 (MQ=255)
                  ggCGTACCACGTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACTCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTgg                                                                                                            <  4:33652/136‑1 (MQ=255)
                    ggTACCACCTACTTCCGCGGCGACCCCACCGAGGTGTTCAACGCGCCGGAGGAGCGCTGGTATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGa                                                                                                          <  3:262162/136‑1 (MQ=255)
                      tACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGAtt                                                                                                        <  7:6982/137‑1 (MQ=255)
                      aaccaccTACTTCCGCGCCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGAtt                                                                                                        <  1:202632/136‑1 (MQ=255)
                        ccaccTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTAc                                                                                                      <  4:135/137‑1 (MQ=255)
                             aaCTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCa                                                                                                 <  5:462281/136‑1 (MQ=255)
                                   cgcgGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTTGGTTACCCGGTTGTGGGGTTACATCCACGACtt                                                                                           >  3:136298/1‑137 (MQ=255)
                                      ggcgACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTGGGTTTACGGGTTGTGGGGTTACATACACTACgtgtg                                                                                        >  3:444930/1‑136 (MQ=255)
                                        cgACCAGACGGTGGTGGTCATCGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTGAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTg                                                                                      <  4:437644/137‑1 (MQ=255)
                                        cgACCACCCCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTg                                                                                      <  3:17656/137‑1 (MQ=255)
                                        cgACCACCCCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTg                                                                                      <  3:17653/137‑1 (MQ=255)
                                        cgACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGGTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTg                                                                                      <  7:448647/137‑1 (MQ=255)
                                             acacCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAg                                                                                 <  4:37838/137‑1 (MQ=255)
                                             acacCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAg                                                                                 <  6:165231/137‑1 (MQ=255)
                                                ccGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGc                                                                              <  5:239672/137‑1 (MQ=255)
                                                  gAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCt                                                                            <  3:361057/137‑1 (MQ=255)
                                                           aaCGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCggg                                                                                                                  <  7:132467/90‑1 (MQ=255)
                                                           aaCGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCggg                                                                                                                  >  8:132467/1‑90 (MQ=255)
                                                           aaCGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTc                                                                   <  5:316412/137‑1 (MQ=255)
                                                            aCGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTTCCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTcc                                                                  >  7:381361/1‑137 (MQ=255)
                                                                     aggagCGCTGGGATGTTTCGCTCACGCCATAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTActgg                                                         <  5:46819/137‑1 (MQ=255)
                                                                     aggagCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACAGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTActgg                                                         <  5:41181/137‑1 (MQ=255)
                                                                           gcTGGGATGTTTCGCGCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCg                                                   <  2:164623/137‑1 (MQ=255)
                                                                               ggATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTCGATTACCGGGTTGTGGGATTACATAAACTACTTGTGTGATAAGGGCCTGAACAAATACCGCTACTGGCTGGCGTTCt                                               >  6:104999/1‑137 (MQ=255)
                                                                                  tGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGa                                            <  5:229475/137‑1 (MQ=255)
                                                                                       cGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCGTTAGATTTACGGGTTTTTGGGTTTCATCCACTACTTTTATTTTGAAGGCCTGAAAAACTCCCCCTACTGGGTGGGGTTGTGGACCaaa                                       >  4:24811/1‑136 (MQ=255)
                                                                                       cGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCACCGGAGTCGCTTTAGATTACAGGGTTGTGGGCTTACATCCACGACATGTCTGTTAAGGGCCTGAACCACACCCACTACTGGCGGGCGTTCTGGGCCacc                                       >  8:246459/1‑135 (MQ=255)
                                                                                                          aaaTACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTaa                    <  7:188479/136‑1 (MQ=255)
                                                                                                          aaaTACCGTCCTCCTCGCGCCGGAGTCGCTTTCGATTCCCGGGTTGGGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTaa                    <  4:29615/136‑1 (MQ=255)
                                                                                                           aaTACCGTGATCCTCGCGCCGGAGTCGCTTTGGCTTACCGGGTTGTGGGATTACATCCACTACGTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTGTGGAACAAGGTGGTGCAGCCGATGGTaaa                   >  4:112839/1‑136 (MQ=255)
                                                                                                           aaTACCGTGATCCTCGCGCCGGAGTCGCTTTGGATTAACGGGTTGTGGGTTTACATCCACTACTTGTGGGTTCAGGGGCTGAACACCTCCCGCGACTGGCTGGCGTTGTGGGCCAACGTGCGGCAGCCGCTGGTaag                   >  4:277537/1‑136 (MQ=255)
                                                                                                           aaTACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTTAATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGTCCAAGGTGCTGCAGCCGATGGTaaa                   >  7:61387/1‑136 (MQ=255)
                                                                                                           aaTACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAAc                   <  4:223612/137‑1 (MQ=255)
                                                                                                           aaTACCGTGATCCTCGCGCCGGAGTCGCGTTGGTTTAACGGGGTTTTGGGTTAAATACAACAAGTGTGTTATAAGGGGCTGAACAAAAACCGCGACTGGGTGGCGTTGTGGTCAAAGGTGGTGGAGCCGAAgggata                   >  2:445466/1‑133 (MQ=255)
                                                                                                           aaTACCGTGATCCTCGCGCCGGAGTCGCGTTGGATTACCCGGGTGTGGGTTTACAAACACAAATTGTGTGATAAGGGCCAGAAAAACACCCGCTAATTGCTGGGGGTGTGGACCAAGGTGCTGCAGCCGCTGGTTAc                   >  8:419000/1‑137 (MQ=255)
                                                                                                           aaTACCGTGATCCTCGCGCCGGAGTCGCGTTCGCTTAACGGGTTGTGGGATTTCATCCCCTACTTGTGTGATCAGGGGCTGAACAACACCCGCTACTGGCTGGCGTTGTGGTCCAAGGGGCGGGAGCCGATGGTAAc                   >  4:138838/1‑137 (MQ=255)
                                                                                                           aaTACCGTGATCCTCGCGCCGGAGTCGCGTTCGATTTACGGGTTTTGGGGTTTAAAACACTACTTGTGTGATCAGGGCCTGAAAAACACCCCCTACTGGCTGGGGTTCTGGACCAAGGTGCTGCAGCCGCTGGttaa                   >  2:309043/1‑134 (MQ=255)
                                                                                                           aaTACCGTGATCCTCGCGCCGGAGTCGCGTTAGTTTAACCGGTTGTGGGATTACAACCACAACTTTGATGAAAAGGGCCTGACCAAATCCCGCTACTGGGGGGCGTTCTGGACACAGGTGCTGCAGCCGCTGGTAAc                   >  8:432215/1‑137 (MQ=255)
                                                                                                           aaTACCGTGATCCTCGCGCCGGAGTCGCGTGGGATTAACGGGTTGTGGGGGTTCATACACAACATTTGGTGTCAGGGCCGGGACAAACCCCGCGACTCGGTGGCGGTAGGGGCCACAGTGGTGCAGCCGCTgggtaa                   >  2:237162/1‑133 (MQ=255)
                                                                                                                ggTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGTATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAAcggcgg              <  6:359546/136‑1 (MQ=255)
                                                                                                                   gATCCTCGCGCCGGAGTCGCGTTGTATTAGCGCGTTTTGGGTTTTAAACCACTACAATTATGTTAAGGGCCAGAACAACAACCCCAACTGGGTGGGGGTGTGGTCCAAGGTGCTGCCGCCCATGGTAACGGCGGccc           >  4:62796/1‑137 (MQ=255)
                                                                                                                     tCCTCGCGCCGGAGTCGCGTTGGGGTAACGGGGTGTGGGGTTTAATACACGACGTGTGTGATGAGGGGCGGAAAAAAACCCGCTACTGGGTGGGGGTTTGGTCCAAAGTGCTGGCGCCGCTGGTTACGGCGGgccgg         >  2:324604/1‑135 (MQ=255)
                                                                                                                     tCCTCGCGCCGGAGTCGCGTTAGATTAACGGGTTGTGGGGTTTCATACACTACTTGTGTGATCAGGGGCTGAACAACTCCCGCTACTTGGGGGCGTTGTGGACCAAGGTGGTGCAGCCCCTGGTTACGGCGGgccgg         >  8:233238/1‑135 (MQ=255)
                                                                                                                          gcgcCGGAGTCGCTTTGGATTACCGGGTTGTGGGATTTCATCCACTACTTGTGTGATCAGGGCCTGAACAACTCCCGCGACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGCTGGTAAAGGCGGCCCGGGTGcg    >  6:189077/1‑136 (MQ=255)
                                                                                                                          gcgcCGGAGTCGCTTTCGATTAGCCGGGTGTGGGTTTACATCCACAAATTGTGTGATCACGGGCGGAACAACACCCGCGACTGGGTGGCGTTGTGGACCAAGGTGGTGCCCCCGATGGTAAAGGCCGCCCGGGTggg    >  2:270157/1‑135 (MQ=255)
                                                                                                                            gcCGGAGGCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTTATCTGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGCCCAAGGTGCTGCAGCCGATGGTAACGGCGGCCCTGGTGCTGa  <  7:150217/137‑1 (MQ=255)
                                                                                                                                       |                                                                                                                             
ACTGGCTGCTCGCCGATGTGCGTACCACCTACTTCCGCGGCGACCACACCGAGGTGGTCAACGCGCCGGAGGAGCGCTGGGATGTTTCGCTCACGCCAGAACTGCTCAATACCGTGATCCTCGCGCCGGAGTCGCTTTCGATTACCGGGTTGTGGGATTACATCCACTACTTGTCTGATCAGGGCCTGAACAACTCCCGCTACTGGCTGGCGTTCTGGACCAAGGTGCTGCAGCCGATGGTAACGGCGGCCCTGGTGCTGA  >  KT2440/1115309‑1115569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑