Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,582,2360CG25.9% 110.0 / 11.3 54R848R (CGC→CGG) CJ019_02433hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (12/28);  new base G (14/0);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 2.98e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTACGAAGAGACCGCCGTATTGGAGCGCTTCCGGGATGAGCTGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGTGAACGATTCTGAATGGCCCCCGGTGATTCCCGTGGGGCCACATTCGGATAACCGAGCCGCATCCCTCC  >  KT2440/2582104‑2582367
                                                                                                                                    |                                                                                                                                   
gTACGAAGAGACCGCCGTATTGGAGCGCTTCCGGGATGAGCTGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCtttt                                                                                                                                 <  3:6197/137‑1 (MQ=255)
             gcctTATTGGAGCGCTTCCGGGAGGAGCTGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATc                                                                                                                    <  6:60241/137‑1 (MQ=255)
             gacggaTTGGAGCGCTTCCGGGATTAGCTTGCCGGACAACTGAGCGACATTGAGGGGGCCAAGCTGGCAGAACTCGCGAAACGCTGCAGCCTGAGGCTGGCATCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATc                                                                                                                    <  8:352487/132‑1 (MQ=255)
                 tATTGGAGCGCTTCCGGGATGAGCTGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGGTTTGCCTGGTGGttt                                                                                                                >  5:470033/1‑136 (MQ=255)
                             tCCGGGATGAGCTGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGaa                                                                                                    <  5:264675/137‑1 (MQ=255)
                              ccGGGATGAGCTGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGGTGTTGGTTTGGCTGCTGGTTCTGATTTAttgtaa                                                                                                   >  3:349744/1‑132 (MQ=255)
                              ccGGGATGAGCTGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGGGTTTTCTTTGCCGGATCGTTGGCATTAATTGaaa                                                                                                   >  3:210454/1‑137 (MQ=255)
                              ccGGGATGAGCTGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTTCCTGTTAGTTATCATTAAAGGaaa                                                                                                   >  7:434028/1‑137 (MQ=255)
                              ccGGGATGAGCTGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGaaa                                                                                                   >  7:122247/1‑137 (MQ=255)
                              ccGGGATGAGCTGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGaaa                                                                                                   >  6:313624/1‑137 (MQ=255)
                                         tGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGCGCTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGGGTTTTGTTTGCCGGATGGGTGGGATAAATAGAAAAAAAcccaaaa                                                                                        >  1:97550/1‑132 (MQ=255)
                                         tGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGGTTTTGGTTTGGCTGATCGTTGTCATTAATGGAAATAAACCATaaa                                                                                        >  6:47163/1‑137 (MQ=255)
                                         tGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGGGTTTTGTTTGGCGGATGGGTCTCATTATATGAAATAAACCAaaaa                                                                                        >  5:279308/1‑137 (MQ=255)
                                         tGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGGGTTTGGTTTGCGGGATCGTTGTGATTTATTGTAATTAAACATaaa                                                                                        >  2:187439/1‑137 (MQ=255)
                                         tGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGGGTTTGGGTTGGCGGATCGTTCTCATTAATGGAAATAAACCATaaa                                                                                        >  8:291656/1‑137 (MQ=255)
                                         tGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGGGTTTGCTGTTGCTGATCGGTGTCAATAATGGAAATAAACCAaaaa                                                                                        >  3:31849/1‑137 (MQ=255)
                                         tGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATaaa                                                                                        <  7:409355/137‑1 (MQ=255)
                                         tGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACACGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGGGGTTGGTTTGGCGGATCGGTGGGATTAATGGGATTAAACCATaaa                                                                                        >  5:58308/1‑137 (MQ=255)
                                          ggCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAg                                                                                       <  6:317975/137‑1 (MQ=255)
                                          ggCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAg                                                                                       <  8:428426/137‑1 (MQ=255)
                                            ccGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGc                                                                                     <  4:177229/137‑1 (MQ=255)
                                            ccGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGc                                                                                     <  5:313624/137‑1 (MQ=255)
                                              gCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGGGTTTGGTTTGCCAGATCGGTGTGATTAATTGAAATAAAACCTAAAGGCaa                                                                                   >  7:268400/1‑137 (MQ=255)
                                              gCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCGTTTGGTTTGGCTGGTCGGTGTCATTTATGGAAATAAACCATAAAGGCaa                                                                                   >  4:9987/1‑137 (MQ=255)
                                                  aaCTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGtt                                                                               <  5:257722/137‑1 (MQ=255)
                                                   aCTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGttt                                                                              <  3:366576/137‑1 (MQ=255)
                                                          gACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGGGTTTTGTTTGGCTGCTCGTTGTCATTAATTGAAATAAACCAAAAAAGCCACCTTTGCATGAg                                                                       >  3:100311/1‑137 (MQ=255)
                                                          gACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCGTTTTCTTTTGCGGATAGGTGTAATTAATTGAAATAAACCAAAAATGCAACGTTTGCATGAg                                                                       >  6:257722/1‑137 (MQ=255)
                                                           aCAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGt                                                                      <  3:9987/137‑1 (MQ=255)
                                                           aCAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGt                                                                      <  2:136682/137‑1 (MQ=255)
                                                           aCAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGt                                                                      <  3:174558/137‑1 (MQ=255)
                                                            cAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCATCCGTAGCAGAGTCTCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGTg                                                                     <  5:269521/137‑1 (MQ=255)
                                                               ggCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCGTTTGCTTTGGCTGATCGGTGTGATTAATTGAAATAAACCATAAAGGAAACGGTTCCATGAGTGaaa                                                                  >  5:162169/1‑136 (MQ=255)
                                                                 cgcgGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGGTTTTGCTTTTGCTGATAGTTCTCATTTATTGAAATAAAACATAAATGCAACGTTTCCATGATTGAAAGa                                                                >  2:116972/1‑137 (MQ=255)
                                                                 cgcgGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCtttt                                                                                                                                 <  3:273948/72‑1 (MQ=255)
                                                                 cgcgGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCtttt                                                                                                                                 >  4:273948/1‑72 (MQ=255)
                                                                 cgcgGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTTCCTGATGGGTGTCATTATTGGTAATAAACCATAAAGGCAACCTTTCCCTGAGTGAACgc                                                                >  8:316282/1‑136 (MQ=255)
                                                                 cgcgGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCGTTTGGTGTGCCGGATCGGTCTCATTAATGGTAATAAACCAAAAAGGCAACGGTTGGAAGAGTggaagc                                                                >  7:56042/1‑132 (MQ=255)
                                                                 cgcgGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCGTTTGCTTTGCCTGAAGGGTGTCATTAATTGAAATAAACCATAAAGGCAACGTATGCATTAGTGCACGa                                                                >  8:457321/1‑137 (MQ=255)
                                                                     ggCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGTGAACGATTCt                                                            <  7:125156/137‑1 (MQ=255)
                                                                     ggCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGTGAACGATTCt                                                            <  6:201110/137‑1 (MQ=255)
                                                                      gCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGTGAACGATTCTg                                                           <  7:291656/137‑1 (MQ=255)
                                                                           gCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGTGAACGATTCTGAATgg                                                      <  1:187439/137‑1 (MQ=255)
                                                                                   aaCTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGGTTTTGGTTTGTCGGATCGTTCTCATTAATGGATATAAACAAAAAATGCCACCTTTACATGAGTGAAAGATTCTTTATTGCCCCCggg                                              >  7:45267/1‑136 (MQ=255)
                                                                                   aaCTCCCGAAACGCGGCAGCCTGAGCCTGGCAGACGTAGCAGAGTCGCGCGTTTTCATTGCCTGATCGTTCTCATTAAATGAAAAAAAACATAAACGCAAACTTTCCATGAATGCACGATTAATAATAGCCACCggg                                              >  8:4233/1‑136 (MQ=255)
                                                                                         cGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGGTTTTGGTTTGGCTGATCGTTGTCATTTATGGTAATAAAACATAAAGGCAAAGGTTCCATTAGTGAAAGATTATGAATGGCCCCCGGCGATTcc                                        >  4:428936/1‑137 (MQ=255)
                                                                                         cGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGTGAACGATTCTGAATGGCCCCCGGTGATTcc                                        <  8:122247/137‑1 (MQ=255)
                                                                                             aCGCGGCAGCCTGATCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGTGAACGATTCTGAATGGCCCCCGGTGATTCCCGTg                                    <  1:5265/137‑1 (MQ=255)
                                                                                                       ctgagcctgGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGTGAACGATTCTGAATGGCCCCCGGTGATTCCCGTGGGGCCACAtt                          <  2:365788/137‑1 (MQ=255)
                                                                                                        tgagcctgGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGTGAACGATTCTGAATGGCCCCCGGTGATTCCCGTGGGGCCACATTc                         <  6:276126/137‑1 (MQ=255)
                                                                                                                      gTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGTGAACGATTCTGAATGGCCCCCGGTGATTCCCGTGGGGCCACATTCGGATAACCGAGCCg           <  6:58308/137‑1 (MQ=255)
                                                                                                                      gTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGTGAACGATTCTGAATGGCCCCCGGTGATTCCCGTGGGGCCACATTCGGATAACCGAGCCg           <  8:21523/137‑1 (MQ=255)
                                                                                                                        aGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGTGAACGATTCTGAATGGCCCCCGGTGATTCCCGTGGGGCCACATTCGGATAACCGAGCCGCa         <  1:32981/137‑1 (MQ=255)
                                                                                                                               gcgcgCTTTTGCTTAGCCTGATCCTTCTCATTAATTGAAATAAACCATAAAGCAAACTTTTCCATGAGTGAAAGATTCTGAATGGCCCCCGGTGATTCCCGTGGGGCCACATTCGGATAACCGAGCCGCATCCCTcc  <  8:345729/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
GTACGAAGAGACCGCCGTATTGGAGCGCTTCCGGGATGAGCTGGCCGCACAACTGAGCGACAAGGCGCGGGCCAAGCTCGCAGAACTCCCGAAACGCGGCAGCCTGAGCCTGGCAGCCGTAGCAGAGTCGCGCTTTTGCTTTGCCTGATCGTTCTCATTAATGGAAATAAACCATAAAGGCAACGTTTCCATGAGTGAACGATTCTGAATGGCCCCCGGTGATTCCCGTGGGGCCACATTCGGATAACCGAGCCGCATCCCTCC  >  KT2440/2582104‑2582367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑