Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,026,5910GC37.8% 47.6 / 10.9 46Q19H (CAG→CAC) ‡CJ019_02841hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (15/13);  new base C (17/0);  total (33/13)
Fisher's exact test for biased strand distribution p-value = 5.79e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGTGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAG  >  KT2440/3026456‑3026726
                                                                                                                                       |                                                                                                                                         
cGTGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAgg                                                                                                                                          >  2:312754/1‑137 (MQ=255)
        cccAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCCGGCCCTGg‑ac                                                                                                                                 >  8:235239/1‑137 (MQ=255)
        cccAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGAGCAACTACGCCGACACCCGGCGCTGCCCCCCCGcccccgcc                                                                                                                                  >  1:288578/1‑132 (MQ=255)
          cAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCCAGCCCAgggccc                                                                                                                                >  6:33484/1‑133 (MQ=255)
          cAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCCCCCGGCCCTGg‑acgc                                                                                                                               >  5:463601/1‑136 (MQ=255)
                    aGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCCCTGCCGCCCCGCCCAGG‑CCGACGTcccgccc                                                                                                                     >  6:376297/1‑135 (MQ=255)
                    aGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATCCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCCGCGCTCCCCCCACGCCCAAG‑AACACGTccagaca                                                                                                                     >  6:124481/1‑134 (MQ=255)
                        ccTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTccagcgccagc                                                                                                                 <  6:341675/137‑1 (MQ=255)
                          tctATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTTCCGCCACGCCCGGG‑GCCCCCTcccgcgccaacgc                                                                                                               >  8:243337/1‑136 (MQ=255)
                          tctATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCCCGCCCAGG‑GCGCCGTCCCGCGCCCGCgg                                                                                                               >  8:270642/1‑137 (MQ=255)
                            tataTCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTCCAGCGCCAGCGGcc                                                                                                             <  8:69109/137‑1 (MQ=255)
                             atatCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTAAGCCGACACCCGGCGCTGCCGCCCCGCCCCCG‑CCCACCTCCCCCGCCACCGGCCg                                                                                                            >  5:8910/1‑137 (MQ=255)
                                    ccgTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTCCAGCGCCAGCGGCCGAT‑CCggg                                                                                                    <  2:10067/137‑1 (MQ=255)
                                       tttCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCACGCCCTGG‑ACCACGTCCCCCCCCACCCGCCGAA‑CCGGgtgt                                                                                                 >  3:265638/1‑137 (MQ=255)
                                           ggCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGAt                                                                                             <  4:53466/137‑1 (MQ=255)
                                                    ttCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCCCGGCCTGG‑AGCACCTCCCCCCCCACCCGCCGCT‑CCGGGTGTTGATGCACAGTgg                                                                                    >  3:253757/1‑137 (MQ=255)
                                                            gATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCCCCACGCCCTGG‑ACGACGTCCAACGCCACCGGCCGAT‑ACGGGTGTTGAAGCCCGGTGGCCGCCAGt                                                                            >  7:140622/1‑137 (MQ=255)
                                                                 aaGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGGACCCCCGCCCAGG‑GCGCCCTGCCCCGCCCGCGGCCGCA‑CCGGGGGGTGTTGCACGGGGGGACCCAGgttgat                                                                       >  2:172563/1‑134 (MQ=255)
                                                                     agagCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTtggtg                                                                   <  7:270642/137‑1 (MQ=255)
                                                                          aGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑TCGACGTCCAGCGCCCGCGGCCGAT‑CCGGGTGTTGGTGCACGGGTGGAGCCAGTTTGTTGGTGGGGaa                                                              >  7:365136/1‑137 (MQ=255)
                                                                           gACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGC‑ACGCCGTCCAGCGCCAGCGGCCGCT‑CCGGGTGTTGAACCACCGGGGCAGCCACTGACATGGTGGGGaaa                                                             >  8:364462/1‑137 (MQ=255)
                                                                               aTGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCCCCCGGCCCCGG‑ACGCCGGCCCCCGCCAGCGGCCGAG‑ACGGGGGTTGATCCCCGGTGGGCGCCCGGTTTTTTGGGGGGGAATCGa                                                         >  8:145784/1‑137 (MQ=255)
                                                                                    aGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGCCGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCt                                                    >  6:219032/1‑137 (MQ=255)
                                                                                    aGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCACGCCCAGG‑ACGCCGTCCAGCGCCACCGGCCGCA‑CCGGGTGTTGATCCACGGTGGCAGCCCGTTTCTTGGTGGGGAAATCGAAAGCt                                                    >  5:315419/1‑137 (MQ=255)
                                                                                         tGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCCGGCCCAGG‑ACGACGGCCCGCCCCAGCGGCCCCT‑GCGGGTGTTGATCCACCGTGGCCGCCACGTTGGTGGTGGGGCAATCGAAAGCGGGgcg                                               >  6:335581/1‑137 (MQ=255)
                                                                                         tGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCCCGCCCAGG‑CCGCCGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCCCGGTGGCAGCCAGTTTCTTTGTGGGGAAATCGCTAGCTTGgcg                                               >  2:36874/1‑137 (MQ=255)
                                                                                              tgtgCAGGGCGCAACTACGCCGACACCCGGCGCTGGCCCCCCGCCCCGG‑GCGCCCGCCCCCCCCCCCGGCCGCTCCCGGGGGTTGTGCCCCGGGGGC‑GCCACGTTGTTGGGGGGGGAAACGCAAGCTGGGggcgcgc                                          >  8:44558/1‑136 (MQ=255)
                                                                                              tgtgCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGcgcgcgc                                          >  7:375225/1‑136 (MQ=255)
                                                                                              tgtgCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGa                                          <  1:312754/137‑1 (MQ=255)
                                                                                              tgtgCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTCCAGCACCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGcgcgcgc                                          >  1:239321/1‑136 (MQ=255)
                                                                                                 gCAGGGCGCAACTACGCCGACACCCGGCGCTGCCCCCCCGCCCAGG‑ACCCCGGCCCCCGCCAGCCGCCGAT‑ACCGGTGGTGATCCCCGGTGGCAGCCAGGTTGATGGGTGGGAAATAGAAAGCAGGgcgcgcccgcc                                       >  8:403852/1‑132 (MQ=255)
                                                                                                   aGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCCCCCCCAGG‑CCGACGGCCAGCGCCAGCCGCCCAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATg                                     >  3:138261/1‑137 (MQ=255)
                                                                                                   aGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑CCGCCGGCCAGCGCCCGCGGGCGCT‑CCGGGGGTTGATCCACGGTGGCAGCCAGTTTCGTGGTGGGGAAATCGGAAGCTGGGCGCGCGAGGATg                                     >  6:195415/1‑137 (MQ=255)
                                                                                                   aGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATg                                     <  7:51305/137‑1 (MQ=255)
                                                                                                   aGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCGGG‑GCGCCGTCCAGCGCCCGCGGCCGAT‑CCGGGGGGTGGTACCCGGTGGGAGCCCGTTTCTTGGGGGGGGAATAGAAAGCTGGGCGCGGGCGCAtt                                     >  4:111347/1‑136 (MQ=255)
                                                                                                    gggCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATgg                                    <  3:381669/137‑1 (MQ=255)
                                                                                                     ggCGCAACTACGCCGACACCCGGCGCTGCCGCCCGGCCCGGG‑GCGCCGTCCCGCGCCCGCCGGCGCT‑CCCGGGGGTGCTCCCCGGTGGCCGGCCGGTTGTTGGGGGGGGAATGGAAAGCGGGGGGCGCGCGCATggg                                   >  8:337382/1‑136 (MQ=255)
                                                                                                      gcgcAACTACGACGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGgcg                                  <  7:364462/137‑1 (MQ=255)
                                                                                                        gcAACTACGCCGACACCCGGCGCTGCCGCCCGGCCCTGG‑GAGACGTCCCGCCCCAGCGGCCGCT‑CCCGGTGTTGGTACACGGTGGCAGCCCGTTTCTTGGTGGGGAAATAGAAAGCTGGGCGCGCGAGCCTTGCGcc                                >  2:379130/1‑137 (MQ=255)
                                                                                                        gcAACTACGCCGACACCCGGCGCTGCCGCCCCGCCCAGG‑GCGCCGGCCCCCGCCCGCGGGCGCT‑CCGGGGGTTTATACCCGGTGGGAGCCAGTTTATTTGGGGGGGAAAAGAAAACAGGGCGCGCGCGCAATGCGcc                                >  6:128360/1‑137 (MQ=255)
                                                                                                        gcAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTCCCGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGcc                                >  7:217605/1‑137 (MQ=255)
                                                                                                        gcAACTACGCCGACACCCGGCGCTGCCCCCAGGCCCAGG‑CCGCCGTCCCGCGCCAGCGGCCGAT‑CCGGGTGTTGCTCCCCGGTGGCAGCCAGGTTCTTGGTGGGGAAATCGAAAGCTGGGCCCGCGCGCAGTGCGcc                                >  8:428879/1‑137 (MQ=255)
                                                                                                          aaCTACGCCGACACCCGGCGCTTCCCCCACGCCCAGGCCCCACG‑CCACCGCCAGCGCCCCCG‑CCGGGGGTTGGTACCCCGTGGCCGCCAGGTTGTTGGTGGGGTAATCGCAATCTGGGCGCGCGCGCGGAGCGCCgc                              >  6:168090/1‑136 (MQ=255)
                                                                                                          aaCTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGa                              <  8:146538/137‑1 (MQ=255)
                                                                                                          aaCTACGCCGACACCCGGCGCGGCCGCCAGGCCCTGG‑ACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGa                              <  4:269760/137‑1 (MQ=255)
                                                                                                                  cGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGc                      <  8:270217/137‑1 (MQ=255)
                                                                                                                                      aGGCCCTGG‑ACGACGTCCAGCGACAGCGGCCGAT‑CGGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTTGTGCGTTACCCGCCAAACTGGCAg  <  2:288578/137‑1 (MQ=255)
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CGTGGTCGCCCAGGCCATGAAGACCCTCTATATCCGCCGTTTCGGCCAGTGGTTCTGAGCGATGCAAGGAGAGCAGACCATGCGAGTACTGATATGTGCAGGGCGCAACTACGCCGACACCCGGCGCTGCCGCCAGGCCCTGG‑ACGACGTCCAGCGCCAGCGGCCGAT‑CCGGGTGTTGATCCACGGTGGCAGCCAGTTTCTTGGTGGGGAAATCGAAAGCTGGGCGCGCGAGCATGGCGCCGATCTGGTGCGTTACCCGCCAAACTGGCAG  >  KT2440/3026456‑3026726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑