Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,350,3600TG38.5% 56.0 / 11.7 53R28R (CGT→CGG) CJ019_04090hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (7/25);  new base G (20/0);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 7.47e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCAGCGCGCGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGCCACC  >  KT2440/4350226‑4350495
                                                                                                                                      |                                                                                                                                       
tCAGCGCGCGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGttt                                                                                                                                       <  1:121934/137‑1 (MQ=255)
tCAGCGCGCGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGttt                                                                                                                                       <  2:137211/137‑1 (MQ=255)
      cgcgTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGGGCGCCCGGTTGATCTa                                                                                                                                 >  8:259046/1‑137 (MQ=255)
         gTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCt                                                                                                                              <  1:91158/137‑1 (MQ=255)
           aTTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGCCCCGTCGGCGCGGGCGCGGCCGGTTGATATACCTgg                                                                                                                            >  2:91158/1‑137 (MQ=255)
           aTTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTTGATCTaacagg                                                                                                                            >  1:279937/1‑132 (MQ=255)
           aTTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTgg                                                                                                                            >  5:236023/1‑137 (MQ=255)
           aTTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGCCCCTTTGATATACCTgg                                                                                                                            >  5:215195/1‑137 (MQ=255)
           aTTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCCCTGGCGCCACCCGTGCATCGACCTgg                                                                                                                            >  8:88825/1‑137 (MQ=255)
               tCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCTCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGc                                                                                                                        <  4:176426/137‑1 (MQ=255)
               tCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGc                                                                                                                        <  7:259046/137‑1 (MQ=255)
               gccATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGc                                                                                                                        <  3:131903/136‑1 (MQ=255)
                 cATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGGGCGACCGGTTCATCGACCTGGCTGccc                                                                                                                      >  6:425158/1‑136 (MQ=255)
                 cATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGGCCGGTTGATCTAACAGGaatccc                                                                                                                      >  4:190675/1‑132 (MQ=255)
                 cATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGGGCGCCCCGTTAAGCTACCTGGATGccc                                                                                                                      >  2:63008/1‑136 (MQ=255)
                    tGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTTGCGCGCCCGGTTCATCTACCTGGATGCCGATg                                                                                                                   >  6:69938/1‑137 (MQ=255)
                      aTCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTTTGATCTAACTGGATGGCGCTGGc                                                                                                                 >  4:447505/1‑137 (MQ=255)
                      aTCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTTTCATCTACCTGGATGCCGATGGc                                                                                                                 >  6:357428/1‑137 (MQ=255)
                      aTCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGATTAATGTACCTGGATGCCCCTGGc                                                                                                                 >  6:457865/1‑137 (MQ=255)
                            gACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGGTGGCGCGGCCGGGTGATAGACCAGGGTGACGATGGCCAAccg                                                                                                           >  3:374393/1‑135 (MQ=255)
                            gACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTTGATGGACCAGGATGCCGCTGGCCAAccg                                                                                                           >  7:15822/1‑135 (MQ=255)
                            gACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGGGCGCCCGGTTAATCGAACAGGATGCCCCTGGCCAAccg                                                                                                           >  7:76491/1‑135 (MQ=255)
                                   tCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTTTCATCTAACTGGCTGCCGAAGGCCCACCGGTAagcg                                                                                                    >  2:258512/1‑137 (MQ=255)
                                    ggtggCCCCCCGCCAATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACCTTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTTGATGCCGATGGCCAACGGGTACGCGa                                                                                                   <  2:229568/134‑1 (MQ=255)
                                     gAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTTGATCTACCTGGCTGCCGATGGCCAACGGGGTCGCGAc                                                                                                  >  7:457371/1‑137 (MQ=255)
                                      aGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACa                                                                                                 <  6:184789/137‑1 (MQ=255)
                                               gccCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGGCCCGTTCATCTACCAGGATGGCGCTGGCCAAACGGGACGCGACCCCGAAAccc                                                                                        >  8:123294/1‑137 (MQ=255)
                                               gccCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGGGCGGCCGGTTTTTCTTCCATGATGACGCTGGGCCACCGGTTAGCGCCCGCGAAAccc                                                                                        >  3:274434/1‑137 (MQ=255)
                                                 ccATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCtt                                                                                      <  7:123294/137‑1 (MQ=255)
                                                      cTTGACTGCCCCCTGCCGCGGAGCCTGCACTACGTTGTCGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCGAACGGGTACGCGACAGCGAAACCCTTgcgcg                                                                                 <  1:295599/137‑1 (MQ=255)
                                                        tGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCa                                                                               <  8:20218/137‑1 (MQ=255)
                                                           cTGCCCCGTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATcg                                                                            <  1:283452/137‑1 (MQ=255)
                                                               ccccTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATcgccgc                                                                        <  4:436044/137‑1 (MQ=255)
                                                                  cTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCAtt                                                                     <  6:236023/137‑1 (MQ=255)
                                                                         gcACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATc                                                              <  6:138930/137‑1 (MQ=255)
                                                                              cTGCACTACTTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACTCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATcccccc                                                         <  8:310210/137‑1 (MQ=255)
                                                                              cTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATcccccc                                                         <  8:388757/137‑1 (MQ=255)
                                                                                   cTACGTTGCCGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCt                                                    <  4:276076/137‑1 (MQ=255)
                                                                                   cTACGTTGACGAGAGTCAGCCGGGCCTGACCGGTCGGCGCTGGGGCGACCGTTTCATCTACCTGGATGACGATGGCCAACGGGAACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCt                                                    <  8:1381/137‑1 (MQ=255)
                                                                                   cTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCt                                                    <  1:178592/137‑1 (MQ=255)
                                                                                   atACGTTGACGACAGTCAGCCGGGCCTGACGCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGTCAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCt                                                    <  7:6124/136‑1 (MQ=255)
                                                                                    taggTTGACGACAGTCAGCCGGGCGTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACGTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTa                                                   <  1:396433/134‑1 (MQ=255)
                                                                                    tACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTTGATCTACCGGGATGCCCATGGCCCACGGGGAAGCGACAGCGCAACCCATGCGCGCCTCGCCGCATTGGTGCTCCCCCCCGCCTa                                                   >  7:117965/1‑137 (MQ=255)
                                                                                      cGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTaca                                                 <  7:368328/137‑1 (MQ=255)
                                                                                      cGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTaca                                                 <  1:52911/137‑1 (MQ=255)
                                                                                        ttGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGACCGGTTCATCTACCTGGATGCCGATGGCCCACCGGTACGCGCCAGCGAAACCCTTGCGCGCCTCGCCGCATTGGTGATCCCCCCGGCCTacccc                                               >  7:227537/1‑134 (MQ=255)
                                                                                          gacgacAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTTTGATCTACCGGGATGCCGAATGCCCACCGGGACGCGCCACCGCAACCCATGCGCGCCTCGCCGCATTGGTGGTCCCCCCCGCCTacaccgc                                             >  6:425213/1‑134 (MQ=255)
                                                                                           acgacAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGAt                                            <  3:347992/137‑1 (MQ=255)
                                                                                              acAGTCAGCCGGGCCTGACCCGTCGGCGCGGGGGCGGCCGGTTGATCTTCCTGGATGGCGCTGGGCAACGGGGTAGCGGCAGCGAAACCCATGCGCGCATCGCCGCCTTTGTGATGCCCCCCGGCTACACGGCtgtg                                         >  4:117244/1‑137 (MQ=255)
                                                                                                aGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGAtgtgtg                                       <  3:447505/137‑1 (MQ=255)
                                                                                                               aCCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATcc                        <  7:463976/137‑1 (MQ=255)
                                                                                                                       gcgcTGGCGCGACCGGTTGATCTAACAGGATTCCGATGGCCAACGGGTACGCGCCAGCGAAACCCATGCGCGCAAAGCCGCAATGGTGATCCCCCCGGCCTACACGGATGTGTGGCTATTCGCCGCTCCGCAAGGcc                >  8:218360/1‑137 (MQ=255)
                                                                                                                       gcgcGGGGGCGGCCGGTTGATCTACCAGGATGGCGCTGGCCCACGGGGTCGCGCCCGCGCAACCCATGCGCCCAACGCCCCATTTGTGGTCCCCCCCGCCAACACGGATGTGTGGATCTGCGCCGCTCCGCAAGGcc                >  6:71557/1‑137 (MQ=255)
                                                                                                                       gcgcGGGCGCGCCCGGTTGATCTACCTGGATGCCGCTGGCCCACCGGTACGCGACCGCGAAACCCATGCGCGCATAGCCGCAATGGTGATACCCCCCGCCTACACCGATGTGTGGATCTGCGCCGATCCCCAACGcc                >  8:463976/1‑137 (MQ=255)
                                                                                                                                     gTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGCCAcc  >  8:130510/1‑137 (MQ=255)
                                                                                                                                      |                                                                                                                                       
TCAGCGCGCGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTTCATCTACCTGGATGCCGATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGCCACC  >  KT2440/4350226‑4350495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑