Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,427,1080CA29.7% 53.7 / 14.3 37intergenic (‑19/‑99)CJ019_04200/CJ019_04201hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (23/3);  new base A (0/11);  total (23/14)
Fisher's exact test for biased strand distribution p-value = 4.26e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCTGCC  >  KT2440/4426983‑4427241
                                                                                                                             |                                                                                                                                     
tgCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAgg                                                                                                                            >  7:192425/1‑137 (MQ=255)
tgCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAgg                                                                                                                            >  3:197447/1‑137 (MQ=255)
 gCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGc                                                                                                                           >  3:333761/1‑137 (MQ=255)
        gAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTa                                                                                                                    >  5:171205/1‑137 (MQ=255)
                 aGTCGGCTTGCCGCCCCGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCCCGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTggcc                                                                                                           >  4:444274/1‑136 (MQ=255)
                 aGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACCACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTggca                                                                                                           >  3:74077/1‑137 (MQ=255)
                 aGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCCCGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGCCTCTAGCACTggcc                                                                                                           >  4:277110/1‑136 (MQ=255)
                 aGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTggca                                                                                                           >  7:291743/1‑137 (MQ=255)
                   gggggttGCCGCCACGCGCCTTTTCCTTGGGCGCAGAACCACACCCGGCCAGCACCAGAACCGCACCCAACGCCAGAATCGCACGCATGTTCCTTCCTGGGGGGCGAAAAAAAGACCGCGACTCTAGCACTggcagg                                                                                                         <  4:162265/132‑1 (MQ=255)
                      gCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCCTCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCAt                                                                                                      >  4:362696/1‑137 (MQ=255)
                                            cATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGGCGCAAAAAAGAAGGTGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACt                                                                                <  5:452448/137‑1 (MQ=255)
                                              tGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTg                                                                              >  5:37811/1‑137 (MQ=255)
                                                    cataaccacaCCCGCCCATCATCACCACCCCACCCAACGGCAGAATCGCACGCTTGTTCCACCCGCGGGGGCCAAAAAACCACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTg                                                                        <  2:107632/131‑1 (MQ=255)
                                                    aaGAAGCACAACCGCCCAGCATCAGCACCGCAGCCACCGGAAAAACCCCCCGCATGTCCCATCCTCGGGGTCGAAAAAAAGAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTg                                                                        <  8:194145/136‑1 (MQ=255)
                                                        aGCACACCCGGACAGCAGCAGCCCCCACGCCAACGGCACAATCCCCCGCTTGTTCCATCGTCGGGGCCCAAAAAACCAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGc                                                                    <  5:380836/137‑1 (MQ=255)
                                                        aGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGc                                                                    >  7:85498/1‑137 (MQ=255)
                                                             ccccGCCCAGCAACAGCCCCGCCCCCACCGCCTGACTCCCCCCCTTGTTCCATCCTCGGGGGCCCAAAACAGCTCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATa                                                               <  6:205832/135‑1 (MQ=255)
                                                                    ccgCATCCGCACCCCACCCAACGTCATAATCGCCCGATGGTTCCATCCTGGGGGGCGAAAAAAACAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATg                                                        <  4:342298/135‑1 (MQ=255)
                                                                                cGCAGCCAACGACAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGa                                            >  2:436670/1‑137 (MQ=255)
                                                                                     ccAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCca                                       >  4:444438/1‑137 (MQ=255)
                                                                                     ccAACGGCAGAATCGCACGCATGTTCCATACTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCAGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCca                                       >  6:112970/1‑137 (MQ=255)
                                                                                               actCGCACGCATGTCCCATCCTCGGGGCCCAAAAAAACAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt                             <  3:300247/135‑1 (MQ=255)
                                                                                               aaTCTCACGCTTGTGACATCCTCGGGGTCAAAAAAAAGACGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt                             <  3:362696/137‑1 (MQ=255)
                                                                                               aaTCGCCCGATTGTTCCCTCCTCGGGGCCCAAAAAAACACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt                             <  8:291743/137‑1 (MQ=255)
                                                                                               aaTCGCACTCATGTTCCATCCTGGGGGTCGAAAAAAACAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt                             <  3:43984/137‑1 (MQ=255)
                                                                                                 ccccacgcaTGTCCCAGCCTCGGGCTCCAAAAAAACACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGc                           <  3:3452/134‑1 (MQ=255)
                                                                                                  cgcacgcaTGTTCCATCCTCGGGGTCACAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCa                          >  4:404840/1‑137 (MQ=255)
                                                                                                      cgcaTGTCCCAACCTCGGGGCCCCAAAAAAGCACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGAAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTAt                      <  3:292604/137‑1 (MQ=255)
                                                                                                                 cccgcGGGGTCGAAAAAAACAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgc           <  5:436104/133‑1 (MQ=255)
                                                                                                                    tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc        >  5:260717/1‑137 (MQ=255)
                                                                                                                    tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc        >  5:260529/1‑137 (MQ=255)
                                                                                                                    tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc        >  5:254893/1‑137 (MQ=255)
                                                                                                                    tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc        >  6:383437/1‑137 (MQ=255)
                                                                                                                    tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc        >  2:428565/1‑137 (MQ=255)
                                                                                                                      ggggTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGccc      >  7:378251/1‑137 (MQ=255)
                                                                                                                       ggcgcccAAAAAAGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCt     <  7:381432/131‑1 (MQ=255)
                                                                                                                        ggTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGACTGAATTGCCGCGCACTTGCCCCTGACGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtc    >  1:330842/1‑136 (MQ=255)
                                                                                                                          tCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtgcc  >  6:33232/1‑137 (MQ=255)
                                                                                                                             |                                                                                                                                     
TGCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCTGCC  >  KT2440/4426983‑4427241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑