Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	5,182,326	A→C	16.5%	L133V (TTG→GTG)	CJ019_04876 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,182,326	0	A	C	16.5%	81.4 / 2.7	56	L133V (TTG→GTG)	CJ019_04876	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (28/17);  new base C (2/9);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGTCGGTAATGCGCTTCTGCAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAGCGCCAACAGATA  >  KT2440/5182192‑5182458                                                                                                                                       |                                                                                                                                     gTTGTCGGTAATGCGCTTCTGCAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCaa                                                                                                                                      <  3:322410/135‑1 (MQ=255) gTTGTCGGTAATGCGCTTCTGAAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCGCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCaa                                                                                                                                      <  1:208895/135‑1 (MQ=255)  ttGTCGGTAATGCGCTTCTGCAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAAc                                                                                                                                     <  6:382849/135‑1 (MQ=255)    gTCGGTAATGCGCTTCTGCAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAAcc                                                                                                                                    >  6:364506/1‑134 (MQ=255)      cGGTAATTCGCTTCTGCAGGATCGAGCCAAAGGTCATGCTGCCCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGAGACCCCGCCCAGGGTGATGCCCTGCTAGACCACCATGATCACGCCGCCCACCCCCa                                                                                                                                 >  2:424255/1‑135 (MQ=255)       ggTAATGCGCTTCTGCAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCACCCCCaa                                                                                                                                >  5:432433/1‑135 (MQ=255)                    tGCTGGATCTAGCATAAGGTCATGCTGACAAACGCCAGCTGGGCGAACAGCAGGCCGGCCAGGTATACCCCGCCCAGGTTGATGCCCTGTTAGACCACCTTGATCAAGCCCCCCAACCCAAAACCCTGGCCGaa                                                                                                                    <  2:317928/134‑1 (MQ=255)                    tGCTGGATCGCGCAAATGGTCATGCTGACCAACGCCAGCCGGGCTAACAGCATGCCCGCCAGCGATACCCCGCCCTGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAACCCCCGGCCGaa                                                                                                                    <  4:137229/134‑1 (MQ=255)                    tGCAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGaa                                                                                                                    >  2:412418/1‑134 (MQ=255)                              aGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCCGCGATACCCCGCCCAGGTTGCTGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTc                                                                                                          >  8:248365/1‑134 (MQ=255)                              aGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCt                                                                                                           >  3:289277/1‑133 (MQ=255)                                      gTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAgcgc                                                                                                 >  1:207204/1‑135 (MQ=255)                                        caggCTGACCAACGCCAGCAGGGCGAACAGCAGGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTgg                                                                                              <  7:354476/133‑1 (MQ=255)                                         aTGCTGACCAACGCCAGCAGGGCGAACAGCAGGCCCGCCAGCGATCCCCCGCCCAGGTTGATGCCCTGGTAGCCCACCATGATCAAGCCGCCCAACCCCAAACCCAGCCCGAACACACGGCTCCAGGAGCGCTgg                                                                                              <  3:426267/135‑1 (MQ=255)                                            cTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGt                                                                                           >  1:340713/1‑135 (MQ=255)                                            cTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCCAACAAACGGCTCCAGGAGCGCTGGCGt                                                                                           >  6:421113/1‑135 (MQ=255)                                              gACCAACGCCAGCGGGGCGAACAGCATGCCCGCCAGCTAAACCCCGCCCTGGTTGAGCCCGTGGTAGCCCACCATGCCCACGCCCCCCACCCCAAACCCCCGCCCCAAAAAACGGCTCCAGGAGCGCTGGCGTTc                                                                                         <  6:324555/135‑1 (MQ=255)                                                ccACCGCCCGCGGGGCTAACCGCAGCCCCGCCCGCGAACCCCCGCCCGGTTGGAGGCCGTGTTACACCACAATCAACACCCCCCCCACCCCCAACCCCCGCCCCAACACACGGCTCCAGGAGCGCTGGCGTTCCa                                                                                       <  2:207204/135‑1 (MQ=255)                                                  aaCGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGCTGCCCTGGTAGCCCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCa                                                                                       >  1:376665/1‑133 (MQ=255)                                                  aaCGCCAGCAGGGCGAACAGCATGCACGCCAGCGATACCCCGCCCAGGTAGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCa                                                                                    >  7:429722/1‑136 (MQ=255)                                                   accccAGCTGGGCGAACAGCAGCCCCGCCAGCCAAACCCCGCCCAGGTGGAGGCCCTGGTATACCACCATGAACAACCCCCCCAACCCCAAACCCCGGCCTAAAAAACGGCTCCAGGAGCGCTGGCGTTCCATCa                                                                                    <  2:129690/132‑1 (MQ=255)                                                       cagcagGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGcacc                                                                               <  8:20373/136‑1 (MQ=255)                                                       cagcagGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGcac                                                                                >  2:205377/1‑135 (MQ=255)                                                          ctgggCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGTTAGACCACCATGATCAAGCCGCCCAACCCCAACCCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACg                                                                            <  7:220186/134‑1 (MQ=255)                                                               cgtagaGCTTGCCCGCCTGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAAGCCCAAACCCAGGCCGAACAAACGGGTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAg                                                                       <  8:237113/131‑1 (MQ=255)                                                               cGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAg                                                                       <  8:66957/136‑1 (MQ=255)                                                                taaCCGCATGCCCGCCTCCTATACCCCGCCCTGGTTGATGCCCTGGTAGACCACCATTATCAACCCCCCAAACCCCAAACCCAGGCCCAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAg                                                                       <  4:49043/134‑1 (MQ=255)                                                                taaCAGCATGCCCGCCCGCGATCCCCCGCCCTGGTGGAGGCCGGGGTAGACCACCATGAACACGCCGCCCACCCCCAACCCCAGGCCCAACACACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAg                                                                       <  2:340713/134‑1 (MQ=255)                                                                gcccAGCAAGCCCGCCCGCGACCCCCCGCCCAGCTTGCCGCCCTGGTCGCCCACAATCATCACGCCGCCCACCCCCAACCCCAGGCCCAACACACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAg                                                                       <  1:362874/132‑1 (MQ=255)                                                                caaCAGCCTGCCCGCCAGCGAACCCCCGCCCCGGTTTCGGCCGTGGTAGACCACCATCAGCAAGCCGCCCACCCCAAAACCCCGGCCGAAAACACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAg                                                                       <  1:412418/134‑1 (MQ=255)                                                                  aCAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGa                                                                     >  4:408586/1‑135 (MQ=255)                                                                       aTGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGt                                                                >  3:242448/1‑135 (MQ=255)                                                                          cccGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGt                                                             >  4:292449/1‑135 (MQ=255)                                                                             gCCCGCGAGACCCCGCCCCGGTTGTGGCCCTGGTCGACCCCCATGATCACGCCGCCCACCCCCAAACCCAGGCCGAACAAACGGCGCCAGGAGCGCTGGCGTTCCATCAGCACCACGGGGAGGATCGGTTGTAccc                                                         <  8:163588/136‑1 (MQ=255)                                                                                   gATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCCCGGGGCGGATCGGTTGTACCCCCATc                                                    >  6:242252/1‑135 (MQ=255)                                                                                   gATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATc                                                    >  6:150205/1‑135 (MQ=255)                                                                                          cGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTgg                                             >  2:87500/1‑135 (MQ=255)                                                                                               aGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAACCCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCCTGGCCATg                                        >  1:124389/1‑135 (MQ=255)                                                                                                      tcccgtGGTAGACCACACTCACCACCCCGCCCAACCCCAACCCCCGCCCCAACACACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCg                                  <  6:340804/129‑1 (MQ=255)                                                                                                         ccTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGGGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCggggg                              >  4:403574/1‑135 (MQ=255)                                                                                                         ccTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCAGGACGCCggggg                              >  4:234551/1‑135 (MQ=255)                                                                                                          cTGGTAGACCACCTTGACAACCCCGCACACCCCCAACCCCAGGCCCACCAACCGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTg                            <  8:235440/136‑1 (MQ=255)                                                                                                             gTAGACCACCATGATCACGCCGCCCACCCCCAAACCCCGCCCCAACACACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACa                         <  7:36932/136‑1 (MQ=255)                                                                                                              tAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACa                         >  6:192921/1‑135 (MQ=255)                                                                                                               aGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAg                                                                       <  6:139809/88‑1 (MQ=255)                                                                                                               aGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAg                                                                       >  5:139809/1‑88 (MQ=255)                                                                                                                  ccaccaTGACCAAGCCGCCCACCCCCAACCCCCGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATggg                     <  2:295331/135‑1 (MQ=255)                                                                                                                      catcatCACGCCGCCCAACCCCAAACCCAGGCCGAACAACCGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATc                 <  5:364506/135‑1 (MQ=255)                                                                                                                      caTGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTAt                  >  5:236268/1‑134 (MQ=255)                                                                                                                      caTGATCAAGCCGACCAACCCCAAACCCAGGCCGAACAAACGGCTCCACGAGCGCTGGCTGTCCAACACCACCACCCTGAGGATCGGTTGTCCCACCCTCACCGTCGACAAGACCCAGGGGGTGACATGGGTAt                  >  6:29548/1‑134 (MQ=255)                                                                                                                      attcaccAACCCGCCCCACCCAAACCCCAGCCCCAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCCCCGTGGCCATGACGCCGGGGGTGACATGGGTATc                 <  3:150204/129‑1 (MQ=255)                                                                                                                        tGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCaa               >  5:377419/1‑135 (MQ=255)                                                                                                                        tGATCAAGCCGCCCAACCCCAAACCCAGGCAGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTAACCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAg              >  7:362506/1‑136 (MQ=255)                                                                                                                         gATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAg              >  3:338928/1‑135 (MQ=255)                                                                                                                            cAAGCCCCCCAACCCCAAACCCAGGCGCAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGTTATCAAGCGcc          <  7:247481/136‑1 (MQ=255)                                                                                                                                 cgccCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGACCATGACGCCGGGGGTGACATGGGTATCAAGCGCCa         >  2:276364/1‑132 (MQ=255)                                                                                                                                  gccCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTg                                                              <  2:312494/78‑1 (MQ=255)                                                                                                                                  gccCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTg                                                              >  1:312494/1‑78 (MQ=255)                                                                                                                                      aaCCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAGCGCCAACAGATa  >  2:325379/1‑134 (MQ=255)                                                                                                                                       |                                                                                                                                     GTTGTCGGTAATGCGCTTCTGCAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAACCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAGCGCCAACAGATA  >  KT2440/5182192‑5182458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑