Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,436,027 T→A 5.3% Q128L (CAG→CTG)  aidB ← Putative acyl‑CoA dehydrogenase AidB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,436,0270TA 5.3% 210.0 / 3.1 76Q128L (CAG→CTG) aidBPutative acyl‑CoA dehydrogenase AidB
Reads supporting (aligned to +/- strand):  ref base T (22/50);  new base A (2/2);  total (24/52)
Fisher's exact test for biased strand distribution p-value = 5.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-01

GCCAACGTTGCGCGGGTCATATTCGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACGCATCAGCTCATGGTAGGCCGGGTGGAACTCGACCAGG  >  KT2440/5435894‑5436161
                                                                                                                                     |                                                                                                                                      
gCCAACGTTGCGCGGGTCATATTCGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTg                                                                                                                                       >  4:302037/1‑135 (MQ=255)
 ccAACGTTGCGCGGGTCATATTCGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTgg                                                                                                                                      <  3:141338/135‑1 (MQ=255)
 ccAACGTTGCGCGGGTCATATTCGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTgg                                                                                                                                      <  3:130779/135‑1 (MQ=255)
  cAACGTTGCGCGGGTCATATTCGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGc                                                                                                                                     <  4:198483/135‑1 (MQ=255)
   aaCGTTGCGCGGGTCATATTCGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCGGCAGGCGCCATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCt                                                                                                                                    <  1:326334/135‑1 (MQ=255)
     cGTTGCGCGGGTCATATTCGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCtgt                                                                                                                                  <  1:178362/135‑1 (MQ=255)
      gTTGCGCGGGTCATATTCGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGc                                                                                                                                <  8:370410/136‑1 (MQ=255)
       ttGCGCGGGTCATATTCGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGc                                                                                                                                <  5:352912/135‑1 (MQ=255)
        tGCGCGGGTCATATTCGCAGGCAAGGATTTTCGGCAGCCTGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCa                                                                                                                               <  6:174556/135‑1 (MQ=255)
           gcgGGTCATATTCGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCaggt                                                                                                                            <  1:20586/135‑1 (MQ=255)
           gcgGGTCATATTCGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCaggt                                                                                                                            <  6:271983/135‑1 (MQ=255)
           gcgGGTCATATTCGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCaggt                                                                                                                            <  3:93323/135‑1 (MQ=255)
                  atatTCGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATc                                                                                                                    >  8:94598/1‑136 (MQ=255)
                       cGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCa                                                                                                                               <  2:178993/120‑1 (MQ=255)
                       cGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCa                                                                                                                               >  1:178993/1‑120 (MQ=255)
                        gccggCAAGTATTTTCGGCCGCCAGTATTCACCCCGCGCCGGCGGCAGGCGCAAGGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGc                                                                                                               <  6:301401/132‑1 (MQ=255)
                        gCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCGGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGc                                                                                                               <  1:423781/135‑1 (MQ=255)
                            gCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGt                                                                                                           >  5:432898/1‑135 (MQ=255)
                            gCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGt                                                                                                           >  5:388440/1‑135 (MQ=255)
                                      tCGGCAGCCAGTATTCAGCCAGCGCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATgg                                                                                                  <  2:350115/134‑1 (MQ=255)
                                       cGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATggg                                                                                                 <  3:141131/134‑1 (MQ=255)
                                           aGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCa                                                                                            <  5:117317/135‑1 (MQ=255)
                                           aGCCAGTATTCAGCAAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCa                                                                                            <  2:402302/135‑1 (MQ=255)
                                             ccAGTATTCCGCCAGCGCCGGCTGCCGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCCGCGGGCAGCCGGTGCCGGCCGCGGCCTGGCTGTGCATGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGc                                                                                          <  3:298576/135‑1 (MQ=255)
                                             ccAGGATGCAGCCCGCCCAGGCGGCCGGCGCAATGCCGGCACGGCGGCAAACGTCATGGGCAGCGGGCATCAGGTGCCGGCCTCGGCCTGGCGGTGCAGGTAGGGCATCGACGCCCGTGCAACATGGGCGCCAGc                                                                                          <  5:29154/135‑1 (MQ=255)
                                                  aTTCAGCCAGCTCAGGCTGCAGGCGAAATGCCGGCACGGCGGCAAAGGTCATGGTCAGCGGGCAGCCGGTGCCGGCGTCGGCCTGGCTGTGCAGGTAGGTCATGGACGCCCGTGCAACATGGGCGCCAGCACggg                                                                                     <  5:377674/135‑1 (MQ=255)
                                                    tCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGt                                                                                   >  3:332555/1‑135 (MQ=255)
                                                       gccagcTCAGGCTGCAGGCGCAATGCCGGAACTGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCg                                                                                <  6:430450/135‑1 (MQ=255)
                                                         cagcCCAGGCTGCTGGCGCAATGCGGGCACGGCGGATAACGTCTTGGGCAGGGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGGCAGCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCg                                                                                <  1:371189/133‑1 (MQ=255)
                                                              cAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCAGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATgg                                                                        >  7:252522/1‑136 (MQ=255)
                                                              cAGGCTGCAGGCGAAATTCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCAGGCTGTGCAGGTAGGTCATCGACTCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATgg                                                                        >  7:252526/1‑136 (MQ=255)
                                                                ggCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGt                                                                       <  5:72587/135‑1 (MQ=255)
                                                                     cAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAaaga                                                                  <  3:302037/135‑1 (MQ=255)
                                                                      aGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAaagaa                                                                 <  4:138189/135‑1 (MQ=255)
                                                                      aGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAaaga                                                                  <  6:432898/134‑1 (MQ=255)
                                                                         cgcAATGCCGGCACGGCGGCTAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAaggc                                                              <  1:35137/135‑1 (MQ=255)
                                                                         cgcAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGccc                                                                            >  4:339966/1‑121 (MQ=255)
                                                                         cgcAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGccc                                                                            <  3:339966/121‑1 (MQ=255)
                                                                         cgcAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAaagaag                                                                >  6:76094/1‑133 (MQ=255)
                                                                         cgcAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAaagaag                                                                >  3:398701/1‑133 (MQ=255)
                                                                           cAATGCCGGCACGGCGGCAAACGTCATGGGCCGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCGGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAaggcag                                                            <  2:71757/135‑1 (MQ=255)
                                                                            aaTGACGGCACGGCGGCAACCTTCATGTGAAGAGGGCAGCCGTTGCCGGCCGAGTCCTGGCTGTGCAGTTAGGTCATCTAAGACAGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGaaggaagg                                                           <  6:285935/135‑1 (MQ=255)
                                                                              tGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGcc                                                         >  1:312145/1‑135 (MQ=255)
                                                                                     aCGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCg                                                  >  4:376779/1‑135 (MQ=255)
                                                                                     aCGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGa                                                 <  7:94598/136‑1 (MQ=255)
                                                                                     aCGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCAGCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGa                                                 <  8:283241/136‑1 (MQ=255)
                                                                                      cggcggCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCGCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGGTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGa                                                 <  5:134900/135‑1 (MQ=255)
                                                                                           gcAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGCCGCCCGAGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGACGGCAGGCCATGTTCGAcggcg                                            >  1:159919/1‑135 (MQ=255)
                                                                                           gcAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGt                                                                                                           <  4:404269/72‑1 (MQ=255)
                                                                                           gcAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGt                                                                                                           >  3:404269/1‑72 (MQ=255)
                                                                                           gcAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTc                                                                                 <  1:426287/98‑1 (MQ=255)
                                                                                           gcAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTc                                                                                 >  2:426287/1‑98 (MQ=255)
                                                                                           gcAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGAcggcg                                            >  5:389834/1‑135 (MQ=255)
                                                                                           gcAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGAcggcg                                            >  1:288344/1‑135 (MQ=255)
                                                                                           gcAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGAcggcg                                            <  3:15273/135‑1 (MQ=255)
                                                                                           gcAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGAcggcg                                            >  3:225778/1‑135 (MQ=255)
                                                                                           gcAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGAcggcg                                            >  4:433195/1‑135 (MQ=255)
                                                                                           gcAAACGTCATGGGCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGAcggcg                                            <  3:19003/135‑1 (MQ=255)
                                                                                           gcAAACGGCATGGGCAGCGGGCAGCCGGTGCCGGCGTCGGCCTGGCTGTGCAGGTCGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGAcggcg                                            <  4:270034/135‑1 (MQ=255)
                                                                                            cAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGAcggcg                                            >  4:19003/1‑134 (MQ=255)
                                                                                               aCGTCCTGGGCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACg                                       <  7:301248/136‑1 (MQ=255)
                                                                                               aCGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACg                                       <  7:314440/136‑1 (MQ=255)
                                                                                                cGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACg                                       <  4:164367/135‑1 (MQ=255)
                                                                                                cGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACg                                       <  4:24992/135‑1 (MQ=255)
                                                                                                    aTGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCAGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACGCATCa                                  <  8:252526/136‑1 (MQ=255)
                                                                                                    aTGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCAGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACGCATCa                                  <  8:252522/136‑1 (MQ=255)
                                                                                                     tGGTCAGCGGGCAGCCGGTGCCGGCCTAGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACGCATCa                                  <  6:157853/135‑1 (MQ=255)
                                                                                                      ggTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGCCGGCGGTACGCATCAGc                                >  7:115314/1‑136 (MQ=255)
                                                                                                        tccgCGGCCAGCCGGGGCCGGCGTCGGCCTGGCTGTGCAGGTAGGGCATCGCCGCCCGTGCAACAGGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGACGGCAGGCCATGTTCGACGGCGGTACGCATCAGCt                               <  3:23318/132‑1 (MQ=255)
                                                                                                        gcAGCGGGCCGCCGGGGCCGGCCGCGGCCTGGCTGTGCAGGTCGGGCATCGACGCCCGTGCAACATGGGCGCCAGCCCGGGGTTCGGCCCATGGTAAAGACGGCAGGCCATGTTCGACGGCGGTACGCATCAGCt                               <  3:28882/134‑1 (MQ=255)
                                                                                                           gCGGGCAGCCGGGGCCGGCCTCGGGCTGGCGGTGCAGGTAGGTCATCGTCGCCCGTGCAACATGGGCGCCAGCACGGTGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACGCATCAGCTCAt                            <  6:389834/135‑1 (MQ=255)
                                                                                                             gggCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGAcggcg                                            >  4:441358/1‑117 (MQ=255)
                                                                                                             gggCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGAcggcg                                            <  3:441358/117‑1 (MQ=255)
                                                                                                             gggCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACGCATCAGCTCATg                           >  2:336504/1‑134 (MQ=255)
                                                                                                              ggCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACGCATCAGCTCATGGTa                        <  7:242645/136‑1 (MQ=255)
                                                                                                                   ccGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACGCATCAGCTCATGGTAGGcc                    <  2:402021/135‑1 (MQ=255)
                                                                                                                                    ctGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACGCATCAGCTCATGGTAGGCCGGGTGGAACTCGACCAgg  >  7:224319/1‑136 (MQ=255)
                                                                                                                                    ctGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACGCATCAGCTCATGGTAGGCCGGGTGGAACTCGACCAgg  >  8:373515/1‑136 (MQ=255)
                                                                                                                                    ctGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACGCATCAGCTCATGGTAGGCCGGGTGGAACTCGACCAg   >  1:319762/1‑135 (MQ=255)
                                                                                                                                    ctGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACGCATCAGCTCATGGTAGGCCGGGTGGAACTCGACCAg   >  1:76136/1‑135 (MQ=255)
                                                                                                                                    ctGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGAGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGAGGTACGCTTCAGCACAGGGTAGGCCGGGTGGAACTCGCCCAg   >  6:262457/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                      
GCCAACGTTGCGCGGGTCATATTCGCAGGCAAGGATTTTCGGCAGCCAGTATTCAGCCAGCTCAGGCTGCAGGCGCAATGCCGGCACGGCGGCAAACGTCATGGTCAGCGGGCAGCCGGTGCCGGCCTCGGCCTGGCTGTGCAGGTAGGTCATCGACGCCCGTGCAACATGGGCGCCAGCACGGGGTTCGGCCCATGGTAAAGAAGGCAGGCCATGTTCGACGGCGGTACGCATCAGCTCATGGTAGGCCGGGTGGAACTCGACCAGG  >  KT2440/5435894‑5436161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑