Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	5,652,716	G→T	5.1%	Q314H (CAG→CAT)	COQ3_2 →	Ubiquinone biosynthesis O‑methyltransferase, mitochondrial

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,652,716	0	G	T	5.1%	264.1 / 4.0	78	Q314H (CAG→CAT)	COQ3_2	Ubiquinone biosynthesis O‑methyltransferase, mitochondrial
Reads supporting (aligned to +/- strand):  ref base G (54/20);  new base T (2/2);  total (56/22)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01

AGGGGTGGGCGCGCGAAGCCTGCGGCGACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCATCCGGACAAGATCGCCG  >  KT2440/5652586‑5652849                                                                                                                                   |                                                                                                                                      aGGGGTGGGCGCGCGAAGCCTGCGGCGACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGAt                                                                                                                                     >  2:11031/1‑133 (MQ=255) aGGGGTGGGCGCGCGAAGCCTGCGGCGACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATc                                                                                                                                    >  8:188637/1‑134 (MQ=255)      tGGGCGCGCGAAGCCTGCGGCGACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGCTCCAGGTc                                                                                                                              >  5:327832/1‑135 (MQ=255)           gcgcgAAGCCTGCGGCGACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACCGCCTGTACGTGGCCTTGCGTAACGGTGTCCCGATCCAGGTCCGCCAt                                                                                                                        >  8:426044/1‑136 (MQ=255)           gcgcgAAGCCTGCGGCGACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCa                                                                                                                         >  4:34146/1‑135 (MQ=255)           gcgcgAAGCCTGCGGCGACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCa                                                                                                                         >  4:223623/1‑135 (MQ=255)           gcgcgAAGCCTGCGGCGACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCa                                                                                                                         >  6:438026/1‑135 (MQ=255)                aaGCCTGCGGCGACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACCGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCCttt                                                                                                                      >  4:270857/1‑133 (MQ=255)                aaGCCTGCGGCGACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCAtttt                                                                                                                     >  8:78554/1‑134 (MQ=255)                aaGCCTGCGGCGACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTc                                                                                                                    >  2:52709/1‑135 (MQ=255)                 aGCCTGCGGCGACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCCTGCGGCCAGCCTGTACGGGGGCTTGCGGAACGGGTTCCCGATGCAGGTCCGCCATTTTCa                                                                                                                   >  5:269906/1‑135 (MQ=255)                 aGCCTGCGGCGACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCa                                                                                                                   >  3:149920/1‑135 (MQ=255)                 aGCCTGCGGCGACCGCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCa                                                                                                                   <  5:266305/135‑1 (MQ=255)                         gcgAGCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACgg                                                                                                           <  1:359675/135‑1 (MQ=255)                          cgACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGc                                                                                                          >  4:146575/1‑135 (MQ=255)                          cgACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGc                                                                                                          <  3:223623/135‑1 (MQ=255)                          cgACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGc                                                                                                          >  5:151408/1‑135 (MQ=255)                          cgACCTCTGGCTCGGCTTCGAACAGGACGACCTGTCGCGCTGGGCCAACCCTGCCGGGCTGGCCCATGCGGCCAGCCTGAACGTGGGGTTTCGGTACGGTTTCCAGATCCAGGTCCGCGAATTTCAGcggccggc                                                                                                          >  6:257137/1‑135 (MQ=255)                               tctGGCTGGGCTTCGAACAGGACGACCTGGCGCGCAGGGCCCACGCTGCCCGGCAGGCCCAAGCGGCCCGCCTGTACCTCGGCTTGCGTAACGGATTCCAGATCCAGGTCCCCCAAATTCAGCGGCCGGCTCGCGa                                                                                                    >  7:146794/1‑136 (MQ=255)                                ctggctggGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGa                                                                                                    >  7:66897/1‑135 (MQ=255)                                          ttCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCATATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCAt                                                                                          >  3:125493/1‑135 (MQ=255)                                          ttCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCATATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCAt                                                                                          >  3:132018/1‑135 (MQ=255)                                           tCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTACCGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATc                                                                                         >  1:7567/1‑135 (MQ=255)                                             gAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCg                                                                                        <  4:400309/134‑1 (MQ=255)                                              aaCAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCgg                                                                                       <  4:149920/134‑1 (MQ=255)                                               aCAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCACGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGt                                                                                      >  5:117809/1‑134 (MQ=255)                                                     cgacCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAAtt                                                                                 >  3:439346/1‑133 (MQ=255)                                                      gacCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTc                                                                               >  5:315433/1‑134 (MQ=255)                                                      gacCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTc                                                                               <  6:315433/134‑1 (MQ=255)                                                      gacCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAg                                                                             >  7:384091/1‑136 (MQ=255)                                                       acCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAg                                                                             <  4:101792/135‑1 (MQ=255)                                                           ggCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGCCAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAAc                                                                         >  2:26632/1‑135 (MQ=255)                                                           ggCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCATATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAAc                                                                         <  4:132018/135‑1 (MQ=255)                                                           ggCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGCCGGCTGGCGACATACACCAACGGTAAATTTCAGGCAcc                                                                        >  8:370413/1‑136 (MQ=255)                                                           ggCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAAc                                                                         >  1:297124/1‑135 (MQ=255)                                                             cgcgcTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCATATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAAc                                                                         <  4:125493/133‑1 (MQ=255)                                                                gcTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCg                                                                    <  1:110941/135‑1 (MQ=255)                                                                    ggCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCa                                                                                                                                  >  8:233850/1‑69 (MQ=255)                                                                    ggCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCa                                                                                                                                  <  7:233850/69‑1 (MQ=255)                                                                         aCGCTGCCGGGCGGGCCCATGCGGACAGCCTGTACGTGGGCTTGCTTAACGGGTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCg                                                           <  2:327028/135‑1 (MQ=255)                                                                            cTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAAt                                                        <  4:439346/135‑1 (MQ=255)                                                                            cTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAAt                                                        <  6:91435/135‑1 (MQ=255)                                                                            cTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAAt                                                        >  5:318587/1‑135 (MQ=255)                                                                                cGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATAc                                                      <  6:193126/133‑1 (MQ=255)                                                                                 gggCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGc                                                                                                          >  8:252185/1‑80 (MQ=255)                                                                                 gggCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGc                                                                                                          <  7:252185/80‑1 (MQ=255)                                                                                 gggCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTccc                                                  >  8:233994/1‑136 (MQ=255)                                                                                    cTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCtt                                                <  4:385266/135‑1 (MQ=255)                                                                                          cATGCGGACAGCGTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCAcc                                          <  2:161967/135‑1 (MQ=255)                                                                                          cATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCAcc                                          >  2:404767/1‑135 (MQ=255)                                                                                               ggACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGACTACTCCCTTTTCACCTCCGa                                     >  3:405207/1‑135 (MQ=255)                                                                                               ggACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCAGCGGTAAATTTCAGGCACCTTCGAGATGAGCGAATACTCCCATTACCCCTCCGa                                     >  2:398601/1‑135 (MQ=255)                                                                                                 aCAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGt                                   <  6:327832/135‑1 (MQ=255)                                                                                                    gCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCg                                >  6:443627/1‑135 (MQ=255)                                                                                                        gTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTcc                           >  7:37122/1‑136 (MQ=255)                                                                                                        gTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTc                            >  2:6447/1‑135 (MQ=255)                                                                                                        gTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGCTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTcc                           >  8:388528/1‑136 (MQ=255)                                                                                                          aCGTGGGCTTGCGTAACGGTTTCCAGCTCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTcc                           >  6:25064/1‑134 (MQ=255)                                                                                                            gTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGa                         <  3:270857/134‑1 (MQ=255)                                                                                                            gTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAg                       >  7:231839/1‑136 (MQ=255)                                                                                                                  ttGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGTGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCAt                  <  3:34146/135‑1 (MQ=255)                                                                                                                  ttGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGACGGGCAt                  >  1:214443/1‑135 (MQ=255)                                                                                                                  ttGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCAt                  >  6:256371/1‑135 (MQ=255)                                                                                                                  ttGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCAt                  >  3:16626/1‑135 (MQ=255)                                                                                                                  ttGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCAt                  >  3:119137/1‑135 (MQ=255)                                                                                                                  ttGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCATc                 >  8:31908/1‑136 (MQ=255)                                                                                                                  ttGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCATc                 >  8:7977/1‑136 (MQ=255)                                                                                                                  ttGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGGAAATTTCAGGAACCTTCGAGATGAGCGAATAATCCCTTTTCACCTCCGAGTCAGTGTCCGCAGGGCAt                  >  1:377097/1‑135 (MQ=255)                                                                                                                    gCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCATcc                >  3:150475/1‑135 (MQ=255)                                                                                                                      gTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCATCCgg              >  6:102136/1‑135 (MQ=255)                                                                                                                      gTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCATCCgg              >  5:161454/1‑135 (MQ=255)                                                                                                                      gTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCATCCGGa             >  7:218978/1‑136 (MQ=255)                                                                                                                        aaCGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGAc                                                                                                             <  4:71463/38‑1 (MQ=255)                                                                                                                        aaCGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGAc                                                                                                             >  3:71463/1‑38 (MQ=255)                                                                                                                        aaCGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACAAACGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCATCCGGa             >  3:155571/1‑134 (MQ=255)                                                                                                                             tttCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCATCCGGa             >  6:423937/1‑129 (MQ=255)                                                                                                                                  aGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCATCCGGACAAGATCGCCg  >  4:250307/1‑135 (MQ=255)                                                                                                                                  aGATCCAGGTCCGCAATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCATCCGGACAAGATCGCCg  >  3:296039/1‑135 (MQ=255)                                                                                                                                   |                                                                                                                                      AGGGGTGGGCGCGCGAAGCCTGCGGCGACCTCTGGCTGGGCTTCGAACAGGACGACCTGGCGCGCTGGGCCAACGCTGCCGGGCTGGCCCATGCGGACAGCCTGTACGTGGGCTTGCGTAACGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGGACGGCTGGCGACATACACCATCGGTAAATTTCAGGAACCTTCGAGATGAGCGAATACTCCCTTTTCACCTCCGAGTCCGTGTCCGAAGGGCATCCGGACAAGATCGCCG  >  KT2440/5652586‑5652849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑