Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 832,610 T→A 8.5% E43D (GAA→GAT)  prs ← Ribose‑phosphate pyrophosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440832,6100TA 8.5% 167.2 / 5.0 56E43D (GAA→GAT) prsRibose‑phosphate pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base T (14/37);  new base A (3/2);  total (17/39)
Fisher's exact test for biased strand distribution p-value = 1.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

GGGAATCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCTGACGTACGACACGCCGAGCCAGATCGGGGTTAGCGTTCCCCGTAAAGACCATCATC  >  KT2440/832478‑832739
                                                                                                                                    |                                                                                                                                 
gggAATCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCCGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTc                                                                                                                                 <  8:84228/135‑1 (MQ=255)
 gtaagcACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTc                                                                                                                                 <  2:171758/129‑1 (MQ=255)
 ggAATCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTc                                                                                                                                 <  5:157841/134‑1 (MQ=255)
 ggAATCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTc                                                                                                                                 <  4:367375/134‑1 (MQ=255)
 ggAATCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTc                                                                                                                                 <  1:5255/134‑1 (MQ=255)
 ggAATCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTc                                                                                                                                 <  2:343581/134‑1 (MQ=255)
 ggAATCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTc                                                                                                                                 <  2:244066/134‑1 (MQ=255)
  gAATCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCAtt                                                                                                                              <  7:75910/136‑1 (MQ=255)
   aaTCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCAtt                                                                                                                              >  1:44964/1‑135 (MQ=255)
    aTCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGa                                                                                                                            <  7:127753/136‑1 (MQ=255)
    aTCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTACCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGGTGAATAATGAAAAAGTCTTTACCGCGAACATTTTAATTgc                                                                                                                            >  8:189300/1‑135 (MQ=255)
     tCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGa                                                                                                                            <  1:206589/135‑1 (MQ=255)
     tCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGa                                                                                                                            <  3:251697/135‑1 (MQ=255)
     tCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGa                                                                                                                            <  3:156926/135‑1 (MQ=255)
     tCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGa                                                                                                                            <  1:391769/135‑1 (MQ=255)
     gcACGGCGGTTATTCGGGATGCGGAGGAGCGGCGGCAGGCGTCTGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGa                                                                                                                            <  2:209624/134‑1 (MQ=255)
        cggcggTGAGTCGGGATGCTGAGGAGCGGCGGCCGGCGTCGGCCATCACTACCAGTTCCATCCGGGTATCGGTGGGCGGGGCGCAGGTCCGCTGAATAATGAAAACGTCTTTTCCGCGCACAGTTTCATTGAtc                                                                                                                          >  1:209624/1‑134 (MQ=255)
           cggTGATTCGGGATGCTGAGGCGCGGCGGAAGGCGTCGGCCATCACGACCAGTTCCATCAGGTTATCGGTGGGCGGGGCGCAGGTCGGCGGAATAATGAAAACGTCTTTACCGCGAACATTTTCAGTGATCTCAg                                                                                                                      >  5:264194/1‑135 (MQ=255)
           cggTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCa                                                                                                                       >  1:48120/1‑134 (MQ=255)
                                     cggAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGCTCTCGCCGTCGGAGAATTTa                                                                                            >  1:440128/1‑135 (MQ=255)
                                                 gCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGCGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACg                                                                                <  2:428738/135‑1 (MQ=255)
                                                    aTCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGGCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCAc                                                                            <  8:49659/136‑1 (MQ=255)
                                                     tCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGTTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCAc                                                                            <  4:265476/135‑1 (MQ=255)
                                                     tCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCAc                                                                            <  1:287394/135‑1 (MQ=255)
                                                     tCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCAc                                                                            <  6:73130/135‑1 (MQ=255)
                                                     tCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCAc                                                                            <  4:390308/135‑1 (MQ=255)
                                                     tCACTACCAGTTCCATCAGGTTATCGTTGGGCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCAc                                                                            <  1:142976/135‑1 (MQ=255)
                                                     tCACTACCAGTTCCATCAGGTTATCGTGGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCAc                                                                            <  4:265480/135‑1 (MQ=255)
                                                     tCACTACCAGTTCCATCAGGGTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCAc                                                                            <  2:233879/135‑1 (MQ=255)
                                                         tACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACTTCTTTACAGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCTACAGAGACGTCACCg                                                                          >  2:302964/1‑133 (MQ=255)
                                                         tACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACTTCACCg                                                                          >  6:273140/1‑133 (MQ=255)
                                                                   aTCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGc                                                              <  6:13539/135‑1 (MQ=255)
                                                                       ggTTATCGTTGGTCGGGGCGCAGGGCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCt                                                          <  2:422916/135‑1 (MQ=255)
                                                                                     ggggCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCTGACGTACGACACGc                                            >  6:190916/1‑135 (MQ=255)
                                                                                     ggggCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCTGACGTACGACACGc                                            >  5:39340/1‑135 (MQ=255)
                                                                                      tggCGCAGGTCGGCTGAATAATGAAAAAGTCTTTACCGCGAACTTTTTCATTGATCTCAGTACTGATCTCGACGGCGGAGAATTTACCGACAGAGACGTCTCCGAGAGGGTTATGCAGCTGTCGTACGTCACGCCg                                          <  8:384843/135‑1 (MQ=255)
                                                                                       ggCGAAGTTGGGCTGATTATTTATAACGTCTTTACCGCGAACATTTTCATTGAGCTCTGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCTAGAGGGATATGCAGCTGACGTACGACACGCCg                                          <  2:227913/135‑1 (MQ=255)
                                                                                        gcgcAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCTGACGTACGACACGCCGa                                         <  2:60912/135‑1 (MQ=255)
                                                                                          gcAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCTGACGTACGACACGCCGAg                                        >  3:218506/1‑134 (MQ=255)
                                                                                           ccggccggcggAATACTGAAAACGTCTTGACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCTGACGTACGACACGCCGAGCCa                                     <  7:165137/131‑1 (MQ=255)
                                                                                           aatgTCGGCGTAATAATGAAAAACTCTTTACCTCGAACATTTTCATTGATCTAAGTACTGATCTCTACGTAGGAGAATTGAACGACAGAGACGTCACCGAGAGGGATATGAAGCTGACGTACGACACGCCTAGCCa                                     <  8:169218/133‑1 (MQ=255)
                                                                                            agtgcGGCTTAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCTGACGTACGACACGCCGAGCCa                                     <  5:270385/131‑1 (MQ=255)
                                                                                            aGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCTGACGTACGACACGCCGAGCCa                                     <  2:44964/135‑1 (MQ=255)
                                                                                            aGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCTGACGTACGACACGCCGAGCCa                                     <  5:434460/135‑1 (MQ=255)
                                                                                                 ggCTGAATAATGAAAACGTCTATACCGCGAACATTATCATAGCTCACAGTACTGATATCTCCGTCGGAGAATTTACCGACACAGACGTCAACTAGAGGGATATGCAGCTGACGTACGACACGCCGAGCCAGATCg                                >  5:360724/1‑135 (MQ=255)
                                                                                                        taatGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCTGACGTACGACACGCCGAGCCAGATCGGGGTTAGc                        <  7:405633/136‑1 (MQ=255)
                                                                                                         aatGAAAACGTCTTTACCGCGAACATTATCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCga                                                                         <  6:152579/86‑1 (MQ=255)
                                                                                                         aatGAAAACGTCTTTACCGCGAACATTATCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCga                                                                         <  6:160513/86‑1 (MQ=255)
                                                                                                         aatGAAAACGTCTTTACCGCGAACATTATCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCga                                                                         >  5:152579/1‑86 (MQ=255)
                                                                                                         aatGAAAACGTCTTTACCGCGAACATTATCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCga                                                                         >  5:160513/1‑86 (MQ=255)
                                                                                                           tGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCTGACGTACGACACGCCGAGCCAGATCGGGGTTAGCg                       <  4:229609/134‑1 (MQ=255)
                                                                                                                aCGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAg                                                            >  3:133698/1‑92 (MQ=255)
                                                                                                                aCGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAg                                                            <  4:133698/92‑1 (MQ=255)
                                                                                                                aCGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCTGACGTACGACACGCCGAGCCAGATCGGGGTTAGCGTTcccc                 >  2:239622/1‑135 (MQ=255)
                                                                                                                         ccGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCTGACGTACGACACGCCGAGCCAGATCGGGGTTAGCGTTCCCCGTAAAGAc         <  1:295092/134‑1 (MQ=255)
                                                                                                                               aCATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCTGACGTACGACACGCCGAGCCAGATCGGGGTTAGCGTTCCCCGTAAAGACCatcatc  >  4:36070/1‑135 (MQ=255)
                                                                                                                                    |                                                                                                                                 
GGGAATCACGGCGGTGATTCGGGATGCTGAGGAGCGGCGGAAGGCGTCGGCCATCACTACCAGTTCCATCAGGTTATCGTTGGTCGGGGCGCAGGTCGGCTGAATAATGAAAACGTCTTTACCGCGAACATTTTCATTGATCTCAGTACTGATCTCGCCGTCGGAGAATTTACCGACAGAGACGTCACCGAGAGGGATATGCAGCTGACGTACGACACGCCGAGCCAGATCGGGGTTAGCGTTCCCCGTAAAGACCATCATC  >  KT2440/832478‑832739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑