Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	2,220,656	A→C	25.6%	D38A (GAC→GCC)	tmk_2 →	Thymidylate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	2,220,656	0	A	C	25.6%	105.6 / 2.2	67	D38A (GAC→GCC)	tmk_2	Thymidylate kinase
Reads supporting (aligned to +/- strand):  ref base A (23/23);  new base C (17/3);  total (41/26)
Fisher's exact test for biased strand distribution p-value = 1.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGATTCGAGCAGGAAGGGCAGGCGTTCTTCGAAGCGGTGCGCCAGGCGTACC  >  KT2440/2220523‑2220790                                                                                                                                      |                                                                                                                                       tAAGCAACGTATAATCGGCCTATGGTAACATATACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGa                                                                                                                                        <  2:326635/134‑1 (MQ=255)    ggAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTgc                                                                                                                                   <  7:252097/136‑1 (MQ=255)      aaCGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTgcg                                                                                                                                  <  4:134297/135‑1 (MQ=255)      aaCGTATAATAGGCCTATGTTTACATTAACACAACAACCAATCCAAGACGCCACCTATGCCTATCCGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTgcg                                                                                                                                  <  3:379193/135‑1 (MQ=255)         gTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTAGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCACGGCGCCCTGCGCCCg                                                                                                                              >  7:241824/1‑136 (MQ=255)          tataATAGGCCTATGTATACATTTACTCAAGAACGAATACAAGACGCCACCTAAGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTACCGGGCGACCTGCGCCCg                                                                                                                              >  4:87029/1‑135 (MQ=255)            taattGGCTTTTGTTTAATTTTACACAACAACGAAACCAACACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCgg                                                                                                                             <  2:369353/134‑1 (MQ=255)                 gcccTATTTTAACATTTACCAACCAACGAACACACCCCGCCACCTATGCCTATCAGGGCGGCGGTCGTGGGCTGTCGGGCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGa                                                                                                                       <  5:82217/133‑1 (MQ=255)                  gCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATAGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGAc                                                                                                                      >  5:217102/1‑135 (MQ=255)                     tATGTTTACATTTACTCAAGGACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGGACAATTCGTACAGGGCGTCCTGCGCCCGGACCAGACCCt                                                                                                                   >  4:371168/1‑135 (MQ=255)                     tATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTGTTTGGGTGGAGCGTATCGCGGTTATGGTACAATTAGTCCAGGGCGCCCGGCCCCCCGACCTGcccca                                                                                                                   >  1:369353/1‑134 (MQ=255)                     tATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGCCCTGCGCCCGGCCCTGCCCCTg                                                                                                                  >  8:252248/1‑136 (MQ=255)                     tATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTACACGGCGACCTGCGCCCGGACCTGcccca                                                                                                                   >  6:434035/1‑134 (MQ=255)                      aTGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTGTGTCGGTCGAGCGGATCGCGGGTCTGGTACAATTCGTCCAGGGCGCCCTGCGCCCGGCCCGGccccgg                                                                                                                  >  1:391587/1‑133 (MQ=255)                       tGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGCGCGTATCGCGGTTCGGGAACAATTCGTCCAGGGCGCCCAGCGCCCGGCCCTGACCCTGGt                                                                                                                >  8:75736/1‑136 (MQ=255)                       tGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCTCCGTTCTTGAAAAATTAGTCCAAGGCCACCAGCGCCCAGACCGGCCCCTgg                                                                                                                 >  7:85520/1‑135 (MQ=255)                       tGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTgg                                                                                                                 >  4:313528/1‑135 (MQ=255)                        gTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGGCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGt                                                                                                                >  6:427515/1‑135 (MQ=255)                         tttACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGCACAAATAGTCCAGGCCGACCGGCGCCCGGCCCTGCCCCTGGTc                                                                                                               >  1:27011/1‑135 (MQ=255)                         tttACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCt                                                                                                              >  8:245150/1‑136 (MQ=255)                          ttACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTGTGTGGGTCGAGCGGATAGCGGGTCTGGAACAAATAGTCCAGGGCGACCAGCGCCCCGACCTGTCCCAGGTc                                                                                                               >  4:244327/1‑134 (MQ=255)                          ttACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTGTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGCCCTGCGCCCGGCCCTGCCCCTGGTCt                                                                                                              >  2:371855/1‑135 (MQ=255)                          ttACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTTTCGGTCGAGCGTTTCGCGGTTCTGGAACAATTCGTACAGGGCGCCCTGCGCCCGGACCTGCACCAGGTCt                                                                                                              >  3:140145/1‑135 (MQ=255)                          ttACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCCGGGCGACCTGCGCCCGGCCCTGACCCTGGTCt                                                                                                              >  3:374346/1‑135 (MQ=255)                          ttACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGa                                                                                                                                        >  7:261724/1‑109 (MQ=255)                          ttACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGa                                                                                                                                        <  8:261724/109‑1 (MQ=255)                          ttACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGCCCTGCGCCCGGACCTGACCCTGGTCt                                                                                                              >  2:90759/1‑135 (MQ=255)                          ttACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAAATCGTCCAGGGCGACCTGCGCCCGGCCCTGACCCTGGTCt                                                                                                              >  3:216947/1‑135 (MQ=255)                          ttACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCGGTCGGTCGAGCGTTTCGCGGTTCTGGAACAATTCGTCCAGGGCGCCCTGCGCCCGGACCTGCCCCAGGTCt                                                                                                              >  2:355627/1‑135 (MQ=255)                              aTTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGa                                                                                                          <  2:19277/135‑1 (MQ=255)                              aTTAACTCAAGAACGAATACAAGCCGCCACCTATGACTAGCAGGGCGGCGGTCGTGGTCTGTCGGGCGAGCGTATCGCGGTTCTGCAACAATTCGTCCAGGGCGCCCTGCGCCCGGACCTGACCCTGGTCTTCGa                                                                                                          <  4:331976/135‑1 (MQ=255)                               tttACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTgc                                                                                                                                   >  8:264942/1‑109 (MQ=255)                               tttACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTgc                                                                                                                                   <  7:264942/109‑1 (MQ=255)                                 aaCTCACGCACGAATACATCACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGGCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGAcc                                                                                                        <  3:371168/133‑1 (MQ=255)                                    tCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGCGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGCCCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGcc                                                                                                    >  2:61838/1‑135 (MQ=255)                                    tCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCCGGGCGCCCTGCGCCCGGACCTGCCCCTGGTCTTCGACCTGCCg                                                                                                   >  7:341819/1‑136 (MQ=255)                                    tCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGCCCTGCGCCCGGGCCTGACCCTGGTCTTCGACCTGcc                                                                                                    >  4:109988/1‑135 (MQ=255)                                    tCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATAGCGGTTCTGGTACAATTCGTACAGGGCGCCCGGCGCCCGGACCTGACCCGGGGAGTAGACCTGcc                                                                                                    >  4:309963/1‑135 (MQ=255)                                     cAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTAGGTCGAGCGGTTCGCGGTTCTGGAACAAATCCTCCAGGGCGCCCTGCGCCCGGCCCTGCCCCAGGTCTTCGACCTGCCg                                                                                                   >  3:295535/1‑135 (MQ=255)                                        gAACGAATACAAGACGCCACCTATGCCTAACAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTgg                                                                                               <  8:137395/136‑1 (MQ=255)                                              aTACAAGACGCAACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTTTCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTg                                                                                          <  4:18321/135‑1 (MQ=255)                                                aCAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGCGCGTATCGCGGTTCTGGAACAAATAGTCCAGGGCGCCCAGCGCCCGGACCTGCCCCTGGTCTTAGACCTGCCGGGGGAAGTgggg                                                                                       >  7:5171/1‑135 (MQ=255)                                                                     tCAGGGCGGCGGTCGTGGTCTGGCGGGCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGGCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGccc                                                                    <  7:377327/134‑1 (MQ=255)                                                                     tCAGGGCGGCGGTCGTGGGCTGTCGGTCGAGCGTAGCGCGGTGCGGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGccc                                                                    <  7:441999/134‑1 (MQ=255)                                                                      ccgggCGGCGGTCGTGGCCTGGCGGGCGAGCGTATCGCGGTTCTGCAACAATTCGGCCAGGGCGCCCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGccc                                                                    <  1:95414/131‑1 (MQ=255)                                                                      cAGGGCGGCGGTCGTTGGCTGGCGGTCGAGAGTATCGCGTTGCTGTAACAATTCGGCCCGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGGGGAAGTGGGCCTTGCCCGCGCCGCCGccc                                                                    <  4:218327/133‑1 (MQ=255)                                                                      cAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTCTCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGccc                                                                    <  4:200733/133‑1 (MQ=255)                                                                      cAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGccc                                                                    <  4:156506/133‑1 (MQ=255)                                                                      cAGGGCGGCGGTCGTGGTCTGTCGGGCGAGCGTAGCGCGGTTCTGCAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGccc                                                                    <  4:420688/133‑1 (MQ=255)                                                                            ggcggTCGTGGTCTGTGGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGCCCGGCGCCCGGACCGGACCCTGGTCTTCGACCTGCCGGGGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGGGGTGGcc                                                           >  7:430004/1‑136 (MQ=255)                                                                              cggTCGTGGTGTGTCGGTGGAGCGTATCGCGGGTGTGGAACAATTAGTCCAGGGCGCCCGGCGCCCCGCCCTGACCCTGGTCTTCGACCAGCCGGGGGGTGTGGGGCTTGCCCGCGCCGCCGCCCGAGGTGGccg                                                          >  2:203657/1‑134 (MQ=255)                                                                                                    cGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTg                                                                                                                  >  1:185694/1‑57 (MQ=255)                                                                                                    cGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTg                                                                                                                  <  2:185694/57‑1 (MQ=255)                                                                                                       agcgcgGTTCTGGAACAATTCGTCCCGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGATTCGAGCAGGAAGGGCAg                                 <  1:371855/133‑1 (MQ=255)                                                                                                       aTCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGATTCGAGCAGGAAGGGCAg                                 <  1:10801/135‑1 (MQ=255)                                                                                                        tCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGATTCGAGCAGGAAGGGCAGGc                               >  7:24334/1‑136 (MQ=255)                                                                                                        tCGCGGTTCTGGAAAAATTGGTCCTGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGATTCGAGCAGGAAGGGCAg                                 <  3:244327/134‑1 (MQ=255)                                                                                                           cgGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCGGGACCGATTCGAGCAGGAAGGGCAGGCGtt                            >  8:191512/1‑136 (MQ=255)                                                                                                            ggTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGATTCGAGCAGGAAGGGCAGGCGttc                           >  8:396850/1‑136 (MQ=255)                                                                                                                   gAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGATTCGAGCAGGAAGGGCAGGCGTTCTTCGaa                     >  2:26543/1‑135 (MQ=255)                                                                                                                           cGTCCAGGGCGCCCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGATTCGAGCAGGAAGGGCAGGCGTTCTTCGAAGCGGTgcg             <  8:241824/135‑1 (MQ=255)                                                                                                                           cGTCCAGGGCGACCTGCGCCCGGCCCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGATTCGAGCAGGAAGGGCCGGCGGTCTTCGAAGCGGTgcg             >  5:88899/1‑135 (MQ=255)                                                                                                                           cGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCGTGGACCGCATCGACCAGGAAGGGGAGGCGGTCTTCGAAGCGGTgcg             >  1:170686/1‑135 (MQ=255)                                                                                                                           cGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGATTCGAGCAGGAAGGGCAGGCGTTCGTCGAAGCGGTgcg             >  5:104118/1‑135 (MQ=255)                                                                                                                            gTCCCGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGATTCGAGCAGGAAGGGCAGGCGTTCTTCGAAGCGGTGCGcc           <  7:396850/136‑1 (MQ=255)                                                                                                                             tCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGATTCGAGCAGGAAGGGCAGGCGTTCTTCGAAGCGGTGCGcc           <  1:90759/135‑1 (MQ=255)                                                                                                                             tCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGATTCGAGCAGGAAGGGCAGGCGTTCTTCGAAGCGGTGCGcc           <  4:216947/135‑1 (MQ=255)                                                                                                                                aGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGCTTCGAGCAGGAAGGGCAGGCGGTCTTCGAAGCGGTGCGCCAgg        >  3:267985/1‑135 (MQ=255)                                                                                                                                    cGACCTGCGCCCGGACCTGACCCTGGTCTTCGACGTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCGTGGAGCGAGTCGAGCAGGAAGGGCAGGCGTTCTTCGAAGCGGTGCGCCAGGCGTa    >  2:311183/1‑135 (MQ=255)                                                                                                                                     gACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGATTCGAGCAGGAAGGGCAGGCGTTCTTCGCAGCGGTGCGCCAGGCGTAcc  >  7:401151/1‑136 (MQ=255)                                                                                                                                      |                                                                                                                                       TAAGGAACGTATAATAGGCCTATGTTTACATTTACTCAAGAACGAATACAAGACGCCACCTATGCCTATCAGGGCGGCGGTCGTGGTCTGTCGGTCGAGCGTATCGCGGTTCTGGAACAATTCGTCCAGGGCGACCTGCGCCCGGACCTGACCCTGGTCTTCGACCTGCCGGTGGAAGTGGGCCTTGCCCGCGCCGCCGCCCGTGGTCGCCTGGACCGATTCGAGCAGGAAGGGCAGGCGTTCTTCGAAGCGGTGCGCCAGGCGTACC  >  KT2440/2220523‑2220790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑