Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,831,985 G→A 5.5% R199C (CGC→TGC)  frp ← NADPH‑flavin oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,831,9850GA 5.5% 239.7 / 3.3 72R199C (CGC→TGC) frpNADPH‑flavin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (40/28);  new base A (2/2);  total (42/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

TGGACCAGGAACGGTTTTCGCGTTGTTGACGATGTTGGAAGTCGCTCATCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTCCGCCGATGTAA  >  KT2440/2831850‑2832115
                                                                                                                                       |                                                                                                                                  
tggACCAGGAACGGTTTTCGCGTTGTTGACGATGTTGGAAGTCGCTCATCCTTCGGTCATAGGCAGCAACTGAAAACGCCTCTGCCTCGGTGGCCTCATAGCGATCACGGTGAAGCACCACTGATTGCGCCAGGCg                                                                                                                                    >  7:39122/1‑136 (MQ=255)
      aGGAACGGTTTTCGCGTTGTTGACGATGTTGGAAGTCGCTCATCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGt                                                                                                                                   >  7:218778/1‑131 (MQ=255)
      aGGAACGGTTTTCGCGTTGTTGACGATGTTGGAAGTCGCTCATCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGt                                                                                                                                   <  8:218778/131‑1 (MQ=255)
       ggAACGGTTTTCGCGTTGTTGACGATGTTGGAAGTCGCTCATCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGACTCGGTCGCCACATAGCGCTCACGGTGAAGCACCACTGATTGCGCCATGCGTGGCtt                                                                                                                              >  4:272410/1‑135 (MQ=255)
           cGGTTTTCGCGTTGTTGACGATGTTGGAAGTCGCTCATCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGAtct                                                                                                                         >  8:248497/1‑136 (MQ=255)
                ttCGCGTTGTTGACGATGTTGGAAGTCGCTCATCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACTGTTAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTcggc                                                                                                                     <  7:71503/135‑1 (MQ=255)
                ttCGCGTTGTTGACGATGTTGGAAGTCGCTCATCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTcggc                                                                                                                     >  6:159658/1‑135 (MQ=255)
                     gttgttGACGATGTTGGAAGTCGCTCATCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCt                                                                                                                <  5:400880/135‑1 (MQ=255)
                     gttgttGACGAGGTGGGAAGTCGCTCATCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCt                                                                                                                <  5:88422/135‑1 (MQ=255)
                      ttgttgACGATGTTGGAAGTCGCTCATCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCtg                                                                                                               <  2:54708/135‑1 (MQ=255)
                       tgttgACGATGTTGGAAGTCGCTCATCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCtgt                                                                                                              <  2:131032/135‑1 (MQ=255)
                       tgttgACGATGTTGGAAGTCGCTCATCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCtgt                                                                                                              <  2:24771/135‑1 (MQ=255)
                                 ggtGGACGTCGCTCATCCTTCGGCCATACGCAGCAACTGAACCCGCCTCTGCCTCGGTGGCCTCATAGCGCGCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCgg                                                                                                    <  5:58602/133‑1 (MQ=255)
                                   tGGCAGTCGCTCCTCCTTCGGTCATAGGCAGCAACTGAACCCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCgg                                                                                                    <  1:192648/133‑1 (MQ=255)
                                           gCTCATCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGATGCACCACTGATAGCGCCAGGGGAGGCTTGATCTCGGCGGGCTGTGCCGGGACGCGATGACCgcc                                                                                          >  6:295071/1‑133 (MQ=255)
                                           gCTCATCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACg                                                                                         <  7:255180/136‑1 (MQ=255)
                                            cTCATCCTTCGGGCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACg                                                                                         <  4:442279/135‑1 (MQ=255)
                                               aTCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGcaca                                                                                     >  8:220795/1‑136 (MQ=255)
                                               aTCCTTCGGTCATAGGCAGCAACTGAAAACGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCTGATCGGGATGACCGACGc                                                                                        >  1:282837/1‑133 (MQ=255)
                                                 ccTTGGGTCATAGTCATCAATTGAAACCGCCTCTGACTCGGTGGCCTCATAGCGCGCACGTTTAAGCACAAATTATTGCGACAGGCGTGGTTTGATCTCGGCGGGCTGTGACTGATCGGTATGACTGACGCACAt                                                                                    <  6:185380/135‑1 (MQ=255)
                                                              gcagcaACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGc                                                                        >  3:94014/1‑134 (MQ=255)
                                                              gcagcaACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTAGATCTCGGCGGGCTGTGCCGGATCgg                                                                                                    >  1:323954/1‑106 (MQ=255)
                                                              gcagcaACTGAAACCGCATCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCgg                                                                                                    <  2:323954/106‑1 (MQ=255)
                                                                agcaACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCg                                                                       >  4:434512/1‑133 (MQ=255)
                                                                agcaACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCg                                                                       >  4:427371/1‑133 (MQ=255)
                                                                     cTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGTGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAgtgt                                                                >  1:403415/1‑135 (MQ=255)
                                                                     cTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAgtgt                                                                >  2:429825/1‑135 (MQ=255)
                                                                     cTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAgtgt                                                                >  5:73084/1‑135 (MQ=255)
                                                                     cTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAgtgt                                                                >  3:88418/1‑135 (MQ=255)
                                                                       gAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCg                                                              >  2:127967/1‑135 (MQ=255)
                                                                          aCCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGttt                                                           <  6:139078/135‑1 (MQ=255)
                                                                             gCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTgg                                                         >  7:189083/1‑134 (MQ=255)
                                                                                     cTCGGTGGCCTCATAGCGCTCACGGTGAAGCCCCACTGATTGCGCCAGGCGTGGCTTGCTCTCGGCGGGCTGTGCCGGATCGGGATGCCCGCCGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAg                                                >  1:422214/1‑135 (MQ=255)
                                                                                            gCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCgg                                        <  7:418703/136‑1 (MQ=255)
                                                                                             ccTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCgg                                        <  6:206595/135‑1 (MQ=255)
                                                                                             ccTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCgg                                        <  6:42375/135‑1 (MQ=255)
                                                                                              cTCATAGCGCTCAAGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGa                                       <  2:271423/135‑1 (MQ=255)
                                                                                                 aTAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCCCATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACAt                                    >  4:311155/1‑135 (MQ=255)
                                                                                                 aTAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATc                                   >  8:157890/1‑136 (MQ=255)
                                                                                                        tCACGGTGTAGCACCACTGATTGCGCCAGTCGTGGCGTGATCTCGGCGGGCTGTGGCGGTTCGGGATGACCGACGCACATGCCAAATACTGCGATAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTcc                             <  2:192157/135‑1 (MQ=255)
                                                                                                        tCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTcc                             <  1:337178/135‑1 (MQ=255)
                                                                                                        gcACGGTGAAGCACCACTGTTTGCGCCAGGCGGGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGAGCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTcc                             <  2:205686/134‑1 (MQ=255)
                                                                                                         cACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCg                            >  3:294526/1‑135 (MQ=255)
                                                                                                          aCGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCggg                          >  8:104931/1‑136 (MQ=255)
                                                                                                          aCGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCgg                           >  1:26528/1‑135 (MQ=255)
                                                                                                          aCGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCgg                           >  3:215063/1‑135 (MQ=255)
                                                                                                          aCGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCgg                           >  4:423714/1‑135 (MQ=255)
                                                                                                          aCGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCgg                           >  2:297811/1‑135 (MQ=255)
                                                                                                          aCGGTGAAGCACAACTGATTGCGCCAGGCGAGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCgg                           >  4:69452/1‑135 (MQ=255)
                                                                                                           cGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGCCCTCGCTTCCggg                          >  3:17885/1‑135 (MQ=255)
                                                                                                           cGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCggg                          >  2:28431/1‑135 (MQ=255)
                                                                                                             gTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGggtg                        >  2:365961/1‑135 (MQ=255)
                                                                                                              tGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCAGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGggtgg                       <  4:427722/135‑1 (MQ=255)
                                                                                                                  gCACCACTGATTGCGCCAGGCGTGGCGTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTgc                   <  3:410093/135‑1 (MQ=255)
                                                                                                                      cacTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCCAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCAtt              >  8:180620/1‑136 (MQ=255)
                                                                                                                        cTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTc             <  3:427371/135‑1 (MQ=255)
                                                                                                                        cTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTc             <  3:434512/135‑1 (MQ=255)
                                                                                                                         tGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTcc            >  2:409812/1‑135 (MQ=255)
                                                                                                                         tGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTcc            >  6:349004/1‑135 (MQ=255)
                                                                                                                         tGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTc             <  1:439787/134‑1 (MQ=255)
                                                                                                                         tGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTc             >  2:439787/1‑134 (MQ=255)
                                                                                                                           aTTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACg                                                                                         <  2:369628/56‑1 (MQ=255)
                                                                                                                           aTTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACg                                                                                         >  1:369628/1‑56 (MQ=255)
                                                                                                                           aTTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATa                                                                            <  6:299287/69‑1 (MQ=255)
                                                                                                                           aTTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATa                                                                            >  5:299287/1‑69 (MQ=255)
                                                                                                                           aTTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTccgc          >  4:391412/1‑135 (MQ=255)
                                                                                                                           aTTGCGCCAGGCATGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTccgc          >  5:269250/1‑135 (MQ=255)
                                                                                                                           aTTGCGCCAGGCATGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTccgc          >  5:274536/1‑135 (MQ=255)
                                                                                                                              gcgcCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTCCGCCGa       >  4:355472/1‑135 (MQ=255)
                                                                                                                                  cAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTCCGCCGATGt    >  3:79815/1‑134 (MQ=255)
                                                                                                                                   aGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTCCGCCGATGTaa  >  4:302966/1‑135 (MQ=255)
                                                                                                                                   aGGCATGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTCCGCCGATGTaa  <  6:274536/135‑1 (MQ=255)
                                                                                                                                   aGGCATGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTCCGCCGATGTaa  <  6:269250/135‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                  
TGGACCAGGAACGGTTTTCGCGTTGTTGACGATGTTGGAAGTCGCTCATCCTTCGGTCATAGGCAGCAACTGAAACCGCCTCTGCCTCGGTGGCCTCATAGCGCTCACGGTGAAGCACCACTGATTGCGCCAGGCGTGGCTTGATCTCGGCGGGCTGTGCCGGATCGGGATGACCGACGCACATGCCAAATACAGCGAAAGTGTCGTTTGGCAGGCCAAGCTCCTCGGACATCGCTTCCGGGTGGTTGCGCATTCCGCCGATGTAA  >  KT2440/2831850‑2832115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑