Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,245,134 A→C 23.8% L182V (TTG→GTG)  CJ019_03982 ← Crotonyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,245,1340AC23.8% 58.0 / 4.4 50L182V (TTG→GTG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (22/15);  new base C (2/11);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 9.18e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGGTGCGCCGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGC  >  KT2440/4245001‑4245268
                                                                                                                                     |                                                                                                                                      
aGGGTGCGCCGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCCACGGCAGGCAACTGCTCGTCGGCGCCGACTTCGCTGACCCAc                                                                                                                                       >  6:309016/1‑135 (MQ=255)
                     cgTATCAGCCGCTTGGTCTGCGCCTAGGCGAAGGTCGGGCCATTGGCCCGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGGGCAGCCTTCGCTGCCCAACCCGGCGGCCCCGGcgcgcgcgg                                                                                                                 >  7:356650/1‑131 (MQ=255)
                             cgtgttGGTCTGCGAATTGGCGATGGTCGGGCCTTTGGCCTGGCGCCCGGCCTGTTCGGGGGAAACGGAAGGAAAGTGATCGTCGGCGCAGACTTCGCTGACAAACCCGGGGGCCCGGGGGCTATCGGCGCCCca                                                                                                          <  6:134814/131‑1 (MQ=255)
                             ccGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCACGGCGCGCTCGGCGccccc                                                                                                          >  2:305066/1‑134 (MQ=255)
                               gCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCCACTGCTCGTCGGCGCAGACTTCGCTGCCCAACCCGGCGGCCCGGGCGCGGTCGGCGCCCcaca                                                                                                        >  3:184989/1‑135 (MQ=255)
                                    accggcgcATAGGCGAAGGGCGGGCCATGGGCCAGGCGCCCGGCCAGTGCGGCGGCAACGGCAGGCAACGGCTGGGCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACtcgt                                                                                                  <  7:261362/132‑1 (MQ=255)
                                      cTGCGCATAGGCGAAGGGCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACtcgtcg                                                                                                <  8:74250/136‑1 (MQ=255)
                                            aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCAACACCGCGAGGCAc                                                                                          >  7:67247/1‑136 (MQ=255)
                                              aGGCGACGGGCGGGCCATTGGCCAGGCGCCCGGCCTGTGCGGCGGCAACGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCTGACCAACCGGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                         <  3:117037/135‑1 (MQ=255)
                                                 cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGGCGAGGAAccgcc                                                                                      >  3:326644/1‑134 (MQ=255)
                                                 cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAcagca                                                                                      >  4:360671/1‑135 (MQ=255)
                                                  gAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCCCTGACCAACCCGGCGGCCCCGGCGCCCACGGCGCCCCCCACCACGTCGCGGCAccccacg                                                                                    >  7:143212/1‑130 (MQ=255)
                                                          ggCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCGCGGCGGCGCAGACTTCGCTGCCCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTgg                                                                             <  6:343349/135‑1 (MQ=255)
                                                             cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACACGTCGAGGGACCCCCGGCGCGTgggcg                                                                          >  4:381057/1‑132 (MQ=255)
                                                             cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCGCGGCGGCGCAGACTGCGCGGACCAACCCGGCGCCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCt                                                                          <  3:337536/135‑1 (MQ=255)
                                                             cATTGGCCAGGCGCCCGGCCAGTGCGGCGGCAACGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCt                                                                          <  1:89889/135‑1 (MQ=255)
                                                             cATTGCCCCGGCGCCCGGCCTGTCCGCCGCCACCGGCAGGCAACTGCGCGGCGGCGCAGACTTCGCTCCCCACCCCGGCGCCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCt                                                                          <  3:63346/135‑1 (MQ=255)
                                                                   ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGATGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGCACAGCAGGCGCTTGGCCTGCTCGCt                                                                   >  8:355212/1‑136 (MQ=255)
                                                                     aGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGc                                                                 <  8:441855/136‑1 (MQ=255)
                                                                       gcgcCCGGCCTGTTCGGCGGCAACGGCAGGCAACGGCTCGTCGGCGCAGACTGCGCTGACCACCCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                                <  2:287281/135‑1 (MQ=255)
                                                                          cccGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACGCGTCGAGGCACCGCCGGCGCGTGGCCTGCTCGCTGCCgct                                                             >  1:44454/1‑133 (MQ=255)
                                                                          cccGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGTCGAGGGACAGCCGGCGCGTGGCCGGCTCGCGGCCgcg                                                             >  4:337536/1‑133 (MQ=255)
                                                                               ccAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCACCTCGTCGAGGGACCCCCGGCGCGTGGCCGGCTCGCTGGCGCTCAGcc                                                        >  3:359615/1‑135 (MQ=255)
                                                                                           cAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCAACTCGTCGGGGCACACCAGGCGCGTGGCCGGCTCGCTGCCGCTCCGCCGTGGCCGGGGcc                                            >  1:288342/1‑135 (MQ=255)
                                                                                           cAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCAACTCGTCGAGGAACAGCAGGCGCTTGGCCAGCTCGCGGCCGCTCAGCCCGGGCCGGTggc                                            >  4:75444/1‑133 (MQ=255)
                                                                                           cAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCACAACTCGTCGCGGCACACCAGGCGCGTTGCCAGCACGCTGCCCCTCCGCCCGGGCAGGTGCCa                                           >  7:411131/1‑136 (MQ=255)
                                                                                                gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGGCCCGGGCGCGCTCGGGCCCCCCCCACGCGTCGACGGCCACCCCGCGCCGTGCCGGCGCCCTGCCGCTCCGCCGTGGGCGGTGCCAGcgg                                       >  4:267148/1‑133 (MQ=255)
                                                                                                 caggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                     <  8:238191/136‑1 (MQ=255)
                                                                                                       acggcgcgtcgGCGCAGACTTCGCTCCCCACCCCGGCGCCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCgg                                <  1:36711/129‑1 (MQ=255)
                                                                                                       aCTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCCATAGGCGCCCCACAACTCGTCGAGGCACAGCACGCGCTTGGCCTTCGCGCTGCCGCTCAGCCGTGGCAGGTGCCAGCTGGCGCCgg                                >  6:310344/1‑135 (MQ=255)
                                                                                                       aCTGCTCGTCGGAGCAGACTTCGCTGACCAACCCGGAGGCCAGGGCGCGAACGGCGCCCCACAAGTCGTCGAGGAACAGCAGGCCCTTGCCCTGCTCGCTGCCGATCAGCCCTGGCACGTGCCCGCAGGCGCCgg                                >  6:230934/1‑135 (MQ=255)
                                                                                                        cTGCGCGGCGGCGCCGACTTCGCTGACCACCCCGCCGGCCAGGGCTCGAGCGGCGCCCCACAACTCGTCGCGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                              <  7:423818/136‑1 (MQ=255)
                                                                                                         tGCTCTTCGGCGCAGACTTCGCTGCCCAACCCGGGGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                              <  6:92831/135‑1 (MQ=255)
                                                                                                         tGCGCGGCGGCGCAGACTTCGCCGACCACCCCGGCGGCCAGGGCGCGTTCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                              <  2:326536/135‑1 (MQ=255)
                                                                                                         tGCGCGGCGGCGCAGACGGCGCTCACCACCCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                              <  2:139063/135‑1 (MQ=255)
                                                                                                           cTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGCTCGGCGCCCCCCAACTCGTCGAGGCACAGCAGGCGCGTGGCCGGCTCGCGGCCGCTCAGCCGTGGCAGGTGGCCGCTGGCGGCGGCaac                            >  2:138092/1‑133 (MQ=255)
                                                                                                              gtcgGCGCAGACTGCGCGGACCAACCCGGCGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                        <  8:48556/136‑1 (MQ=255)
                                                                                                              ggcgGCCCATACTTCGCTGACCACCCCGGCGGCCAGGGCGCGATCGGGGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                        <  7:51772/134‑1 (MQ=255)
                                                                                                              gccgGCGCAGACTGCGCGGCCCACCCCGGCGGCCAGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                        <  8:55675/134‑1 (MQ=255)
                                                                                                               gcgGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                        <  6:354424/134‑1 (MQ=255)
                                                                                                               gcgGCGCAGACTTCGCGCCCCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                        <  3:381057/134‑1 (MQ=255)
                                                                                                                cgGCGCAGAGTTCGCGTACCAACCGGGCGGCCAGGGGGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCt                      <  8:143212/136‑1 (MQ=255)
                                                                                                                 ggCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGGGCCCCCCAACGCGGGGCGGCCCAGCAGGCGCGTGGCCTGCTCGCGGGCGAGCAGCCGGGGGAGGTGCCAGCGGGCGCCGGCCTCgggggg                      >  4:344798/1‑133 (MQ=255)
                                                                                                                 ggCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGGCGAGGAACAGCAGGCGCTTGGGCTGCTCGCGGGCGATCAGCCGGGGCAGGTGCCCGCGGGCGCCGGCATCgggggg                      >  5:357287/1‑133 (MQ=255)
                                                                                                                      aGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGCACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGcc                >  7:254186/1‑136 (MQ=255)
                                                                                                                        aCTTCGCTGACCACCACGGCGCCAAGTGGGCTAGGGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCTATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc               <  6:110080/135‑1 (MQ=255)
                                                                                                                        aCTGCGCTGCCCAACCCGGCGGCCGGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCCGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc               <  1:87088/135‑1 (MQ=255)
                                                                                                                                gACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCCCAACTCGTCGACGCACAGCACGCGCGTTGCCTGCTCGCTGCCGATAAGCCCTGGCACGTGCCAGCAGGCCCCCGCATAGGGGCGGTAGCCCAAGCGGgt       >  4:57940/1‑135 (MQ=255)
                                                                                                                                 cccACCCGGGCGGCCGGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt     <  7:248269/135‑1 (MQ=255)
                                                                                                                                  ccAACCCGGGGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt     <  2:155353/135‑1 (MQ=255)
                                                                                                                                    aaCCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGc  >  8:51568/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                                      
AGGGTGCGCCGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGC  >  KT2440/4245001‑4245268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑