Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,524,7320TG55.0% 5.9 / 16.7 20Q67P (CAG→CCG) CJ019_01439hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (6/3);  new base G (0/11);  total (6/14)
Fisher's exact test for biased strand distribution p-value = 2.17e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGG  >  KT2440/1524610‑1524863
                                                                                                                          |                                                                                                                                   
aGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAggg                                                                                                                        >  1:163265/1‑136 (MQ=255)
 gggcgcgcgGGCAGCCGGTGGCAACTGGCTTGCCAGCCGGCGCTGCAAGGGCGCCAGGCGCGCGGCCGGGTGCAGCAGCCGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGTAAGGTGGCGGGGCGGGGGAGGGt                                                                                                                       <  7:68330/131‑1 (MQ=255)
     tCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGATGGTGGCTGGGCGGGGGAGGGTTTGt                                                                                                                   >  3:208708/1‑136 (MQ=255)
     tCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTg                                                                                                                    >  3:80501/1‑135 (MQ=255)
       gcgGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAg                                                                                                                 >  5:222025/1‑136 (MQ=255)
         gggCAGCGGTTTGCAATGGGCTTGCCAGCAGGCGATGAAAGTGCTCCAGGCGCGGGGCATGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCTGGGCGTAAGGTGGCGGGGGGGGGGAGGGTTTGTAGGc                                                                                                               <  4:59841/136‑1 (MQ=255)
          ggCAGCCGGTTGCAACTGGCTTTCCAGCAGGGGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGc                                                                                                               >  8:73241/1‑135 (MQ=255)
                   ttGCACCTGGCTTTCCAGCCGGCGCGGCACGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCTGGCGCAGGGGGCGCACCTGGCGCAGCAGGGCGTATGGTGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTa                                                                                                     <  1:194055/136‑1 (MQ=255)
                                    gctgccgcTGCAAGGGCTCCCGGCGCCGGGCCTGGGTCCGCAGCCGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                                    <  6:241090/131‑1 (MQ=255)
                                    gccggggcTGAAAGTGCGCCCGGCGCGGGGCCTGGTGCAGCAGCCGGCGCCGGGGGCGCACCTGGCGCAGCTGGGCGTATGGGGGCGGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                                    <  6:227034/130‑1 (MQ=255)
                                    gcagGCGCTGCAGGTGCGCCTGGGGGGGGGCCGGGTGCAGCAGCGGGCGCTGCGGGGGCACCTGGCGCTGCAGGGCGTAAGGGGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                                    <  2:211681/136‑1 (MQ=255)
                                    gacggCTCTGCAATTGCTCCAGGGGAGCGGCCTGGTTCAGACGCAGGCGCTGCGGGCGCACCTGGGGCAGCAGGGCGTAAGGTGGGTGGGGGGGGGTGGGTGTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                                    <  7:42430/133‑1 (MQ=255)
                                           cTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCCGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGGTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGt                                                                             >  5:163600/1‑136 (MQ=255)
                                                   gCTCCAGGCGGGCGGCCTGGTGCAGCAGCAGGCGCCGGGGGCGCACCTGGCGCAGACGGGCGGAAGGGGGGGGGGCGGGGGAGGGTTTGTCGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACAtc                                                                     <  2:188552/136‑1 (MQ=255)
                                                        aGGCGCCCGGCGGGGTGCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGt                                                                <  4:195644/136‑1 (MQ=255)
                                                                  cgtGGTTCAGCAGCAGGCGCCGCGGGCGCACGTGGCGCCGCCGGGGGTATGGGGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGcc                                                      <  8:150800/134‑1 (MQ=255)
                                                                                      cgCGGGCGCACCGGGCGCCGCTGGGCGTATGGGGGCGGGGGGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAAcc                                  <  2:34258/135‑1 (MQ=255)
                                                                                      cgCGGGCGCACCGGGCGCAGCCGGGCGGAAGGGGGGGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAAcc                                  <  7:214680/135‑1 (MQ=255)
                                                                                             gcACCGGGCGCAGCCGGGCGGAAGGTGGCGGGGCGGGGGAGGGGTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCt                           <  3:195652/136‑1 (MQ=255)
                                                                                                    gcgcAGCGGGGGGGAAGGGGGGGGGGCGGGGGCGGCTGTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAggg                    <  6:138994/136‑1 (MQ=38)
                                                                                                                      gggcggggcggGGGAGGGCGTGGAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAgg  <  1:153286/135‑1 (MQ=255)
                                                                                                                          |                                                                                                                                   
AGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGG  >  KT2440/1524610‑1524863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑