| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,391,026 | 0 | T | G | 58.8% | 3.4 / 11.9 | 19 | Q561P (CAG→CCG) | bvgS | Virulence sensor protein BvgS |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (10/0); total (13/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.66e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCTTTGGAGTT > KT2440/2390905‑2391132 | gTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCGGGGGGATATgccgg > 8:179133/1‑136 (MQ=255)gTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGGTGGGCGCGCGGGCGCGGGGGCATCTgacgg > 7:176217/1‑132 (MQ=255)gTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCCCGCGGTCGGCGCGCTGGCGCAGGCGAAACAgcagg > 1:16347/1‑136 (MQ=255) cAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTgcaggcgcaggc < 7:71817/136‑1 (MQ=255) cAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGGGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTgcaggcgcaggc < 1:46863/136‑1 (MQ=255) tttCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCCGTCGGCGCGCAGGCGCGGGCGAATCTGCAGGCGCCGGCGGGcc > 4:28155/1‑135 (MQ=255) agatacagGGGGGGGGGCGTCCCTTTGAGCCGGGCGCGCATGATCGCCATGGGGCCTGACCGGGCGGGTGCGGCGCGGTGGCGCTGGCGAATCTGCAGGCGCTGGCGGGCATTCCAGCGCAGGGCCAGCAACaaac < 4:158794/134‑2 (MQ=255) catGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTAGGCGCGCGGGCGCAGGCGCATATGGAGGCGCACGCCGGCCATACACAGCAGGGCCAGCCACAAAACCAcc > 8:203554/1‑135 (MQ=255) gtggggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGGTCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCCGGCGGGCCTTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTg > 4:211296/1‑136 (MQ=255) gtggggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTGGAGCGCGCGGGCGGCGCGCGGGCGCGGGCGCCAATGCAGGCGCCCGCCGGGAGTCCAGACCACGGCCACCACCAAAAACACCGcccgg > 2:105219/1‑133 (MQ=255) gtggggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTGCAGAGCGCCTTCGGCGCGCACGCGCGGGGGAAACTGCCGGCGCCCGCGGGCATTACAGAGCAGGGCCAGGACCAAAATCACCGCCATg > 6:47655/1‑136 (MQ=255) ggggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTGAGCGCGCGTTCGGCGCGCGGGCGCGGGCGCATCTGCCGGCGCCCGCGGGGCTTACAGAGCAGGGCCCGCCACAAAAGCACCGCCGGGGc > 6:236081/1‑136 (MQ=255) tACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGGTCGGCGCGCTGGCGCGGGCGCATCTGCAGGCGCAGGCGGGCAGTCCCGAGCAGGGCCAGCAACCAAAGCACCGCCGTGGCCCGccc > 2:152223/1‑134 (MQ=255) aGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGGTCGGCGCGCTGGCGCGGGCGGATCTGCAGGCGCAGGCGGGGATTACAGCGCAGGGGCCGCACCAAAAGCACGGCCGTGGCCCGCCCCCCCAGcc > 2:61799/1‑135 (MQ=255) gcgcATGAATGCCAACTGGTCGTGCAGCGCGCGGGGGGCGCGCGGGCGCGGGGGACTCGGCAGGCGCCCGCGGGCCTTGCAGCGCACGGGCCGCCACAAAAGCCCCGCCGTGGGCCGCGCCCCCCGCCGGGCCCgg > 2:27317/1‑136 (MQ=255) aaggCCACCTGGGGGTTCAGCGGGGGTGCGGCGCGCGGGCGCTGGCGAAGCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGt < 7:30334/133‑1 (MQ=255) gcGTTCAGCGGGGGTGCGGCGCGCGGGCGCTGGCGAAGCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCTTTGGAGt < 7:179133/135‑1 (MQ=255) ggTTCCGCGCGGGGGCGGCGCGGTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCTTTGGAGtt < 2:112007/135‑1 (MQ=255) cGTTCAGCCCGCGTTCGGCGCGCTGGCGCGGGCGAATCTGCAGGCGCCGGCGGGCATTCCCGAGCCGGGCCCGCAACAAAACCACGGCCGTGGCCAGCCCCCCCAGCCGGGCCCGGCGGCGGTTGCCGTTGGAGtg > 8:141772/1‑135 (MQ=255) | GTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCTTTGGAGTT > KT2440/2390905‑2391132 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |