| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,432,605 | 0 | T | G | 52.2% | 8.1 / 18.3 | 23 | S97A (TCG→GCG) | fadA_2 | 3‑ketoacyl‑CoA thiolase |
| Reads supporting (aligned to +/- strand): ref base T (0/11); new base G (9/3); total (9/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.37e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGACGTGATCTGGGGCTGCGTCAACCAGACCCTGGAGCAGGGCTGGAACATCGCCCGCATGGCTTCGCTGATGACCCCGATCCCGCACACCTCTGCGGCGCAGACCGTCAGCCGCCTGTGCGGCTCGTCCATGAGCGCGCTGCACACGGCCGCCCAGGCGATCATGACCGGTAACGGTGATGTGTTCGTGGTCGGTGGCGTGGAGCACATGGGCCACGTCAGCATGATGCATGGCGTAGACCCCAACCCG > KT2440/2432481‑2432730 | ggACGTGATCTGGGGCTGCGTCAACCAGACCCTGGAGCAGGGCTGGAACATCGCCCGCATGGCTTCGCTGATGACCCCGATCCCGCACACCTCTGCGGCGCAGACCGTCAGCCGCCTGTGCGGCTCGTCCATGAgc < 7:136691/136‑1 (MQ=255) gACGTGATCTGGGGCTGCGTCAACCAGACCCTGGAGCAGGGCTGGAACATCGCCCGCATGGCTTCGCTGATGACCCCGATCCCGCACACCTCTGCGGCGCAGACCGTCAGCCGCCTGTGCGGCTCGTCCATGAgc < 2:3019/135‑1 (MQ=255) gggCTGCGTCAACCAGACCCTGGAGCAGGGCTGGAACATCGCCCGCATGGCTTCGCTGATGACCCCGATCCCGCACACCTCTGCGGCGCAGACCGTCAGCCGCCTGTGCGGCTCGTCCATGAGCGCGCTGcaca < 4:102565/134‑1 (MQ=255) cAACCAGACCCTGGAGCAGGGCTGGAACATCGCCCGCATGGCTTCGCTGATGACCCCGATCCCGCACACCTCTGCGGCGCAGACCGTCAGCCCCCAGTGCGGCGCGGCCCAGAGCGCGCGGCACACCGcccccccc > 7:142883/1‑134 (MQ=255) cAACCAGACCCTGGAGAAGGGCTGGAACATCGCCCGCATGGCTTCGCTGATGACCCCGATCCCGCACACCTCTGCGGCGCAGACCGTCAGCCCCCTGGGCGGCGCGTGCCAGAGCGCGCGGCCCCCCGCCGccccg > 4:103035/1‑134 (MQ=255) cccTGGTGCAGGGCTGGAACATCGCCCGCTTGGCTGCGCTGAGGACCCCGATCCCGCACACCTCTGCGGCGCAGACCGTCAGCCGCCTGTGCGGCGCGTCCATGAGCGGGCTGCACACGGCCGCCCAGGCGATCAt < 6:179526/136‑1 (MQ=255) cgggCTGGAACCTCGCCCGAATGGATGGGAGGATTACCCCGAGCCCGCACACCTCTGCGGGGCATCCCGGCTGCGGCCTGTGCGGCTCGGCCATTAGCGCGCTGAACACGGCCGCCCAGGCGATCATGACCGGTaa < 8:96949/135‑1 (MQ=255) aGGGCTGGAACATCGCCCGCATGGCTTCGCTGATGACCCCGATCCCGCACACCTCTGCGGCGCAGACCGGCAGCCGCCTGTGCGGCGCGTCCATGAGCGCGCGGCACACGGCCGCCCAGGCGATCATGACCGGTaa < 2:1353/136‑1 (MQ=255) cTGGAACATCGCCCGCATGGCTTCGCTGATGACCCCGATCCCGCACACCTCTGCGGCGCAGACCGTCAGCCGCCTGTGCGGCTCGTCCATGAGCGCGCTGCACACGGCCGCCCAGGCGATCATGACCGGTAACGGt < 6:1296/136‑1 (MQ=255) tGGAACATCGCCCGCATGGCTTCGCTGATGACCCCGATCCCGCACACCTCTGCGGCGCAGACCGTCAGCCGCCAGGGCGGCGCGGCCCAGAGCGCGCGGCCCACCCCCCCCCAGGCGCGCATGACCGGGaaacggg > 3:65028/1‑131 (MQ=255) ccGCATGGCTTCGCTGATGACCCCGCTCCCGCACCCCTCTGCGGCGCAGACCGTCAGCCCCCTGTGCGGCGAGTCGCCTCGCGCCCGGGCCCCCGCCGCCCCGGCGCTCATGGCCGGGATCGGTGAGGTGTTCggg > 7:249212/1‑134 (MQ=255) ccGCATGGCTTCGCTGATGACCCCGATCCCGCACACCTCTGCGGCGCAGACCGTCAGCCGCCTGTGCGGCGCGTCCATGAGCGCGCGGCCCACCGCCGCCCCGGCGATCATGACCCGGTACGGGGGTGTGTTGGTg > 1:155135/1‑136 (MQ=255) ccGCATGGCTTCGCTGATGACCCCGATCCCGCACACCTCTGCGGCGCAGACCGTCAGCCGCCAGTGGGGGGCGTGCATGAGCGCCCGGCACCCCGCCCCCCCGGCGCTCATGACCCGTATCGGGGGTgtggtggtg > 8:162479/1‑136 (MQ=255) cGCATGGCTTCGCTGATGACCCCGATCCCGCACACCTCTGCGGCGCAGACCGTCAGCCGCCGGGGGGGGGGGGCCCGGCGCGCGCGGCCCCCCGCCGCCCCGGCGCGCAAGGCCGGGAACGGGGGTGtggtggtgg > 4:232925/1‑136 (MQ=255) tgatgaCCCCGATCCCGCACACCTCTGCGGCGCAGACCGTCAGCCCCCGGTGCGGGGCGGGCATGAGCGCGCGGCCCCCGGCCGCCCCCGCGAGCCAGACCGGTAACGGGGGTGTGTTGGTGGTCGgggggggggg > 4:174019/1‑130 (MQ=255) cccGCACACCTCTGCGGCGCAGACCGTCAGCCGCCTGTGCGGCTCGTCCATGAGCGCGCTGCACACGGCCGCCCAGGCGATCATGACCGGTAACGGTGATGTGTTCGTGGTCGGTGGCGTGGAGCACATGGGCCAc < 3:174019/136‑1 (MQ=255) cacaCCTCTGCGGCGCAGACCGTCAGCCGCCTGTGCGGCTCGTCCATGAGCGCGCTGCACACGGCCGCCCAGGCGATCATGACCGTTAACGGTGATGTGTTCGTGGTCGGTGGCGTGGAGCACATGGGCCACGTCa < 2:196853/136‑1 (MQ=255) tGCGGCGCAGACCGTCAGCCGCCGGTGCGGCGCGTGCAAGAGCGCGCGGGCCACGGCCGCCCCCGGGGTCATGTCCGGGTACCGGGGTGTGTTGGGGGGCGGTGGGGGGGGGGCCATGGGCCACGTCCGCatgatg > 2:26273/1‑136 (MQ=255) cgcAGACCGTCAGCCGCCTGTGCGGCTCGTCCATGAGCGCGCTGCACACGGCCGCCCAGGCGATCATGACCGGTAACGGTGATGTGTTCGTGGTCGGTGGCGTGGAGCACATGGGCCACGTCAGCATGATGCATgg < 4:95711/136‑1 (MQ=255) gatgACCGTCAGCCGCCTGTGCGGCTCGTCCATCAGCGCGCTGACCACGGCCGCCCAGGCGATCATGACCGGTAACGGTGATGTGTTCGTGGTCGGTGGCGTGGAGCACATGGGCCACGTCAGCATGATGCATGGc < 1:190428/133‑1 (MQ=255) gTCAGCCGCCTGTGCGGCTCGTCCATGAGCGCGCTGCACACGGCCGCCCAGGCGATCATGACCGGTAACGGTGATGTGTTCGTGGTCGGTGGCGTGGAGCACATGGGCCACGTCAGCATGATGCATGGCGTAGAcc < 5:154893/136‑1 (MQ=255) aGCCGCCTGTGCGGCTCGTCCATGAGCGCGCGGCACACGGCCGCCCAGGCGATCATGACCGGTAACGGTGATGTGTTCGTGGTCGGTGGCGTGGAGCACATGGGCCACGTCAGCATTATGCATGGCGTAGACCCCa < 7:191063/136‑1 (MQ=255) ccTGTGCGGCGCGTCCATGAGCGCGCTGCACACGGCCGCCCAGGCGATCATGACCGGTAACGGTGATGTGTTCGTGGTCGGTGGCGTGGAGCACATGGGCCACGTCAGCATGATGCATGGCGTAGACCCCAACCCg < 3:232925/136‑1 (MQ=255) | GGACGTGATCTGGGGCTGCGTCAACCAGACCCTGGAGCAGGGCTGGAACATCGCCCGCATGGCTTCGCTGATGACCCCGATCCCGCACACCTCTGCGGCGCAGACCGTCAGCCGCCTGTGCGGCTCGTCCATGAGCGCGCTGCACACGGCCGCCCAGGCGATCATGACCGGTAACGGTGATGTGTTCGTGGTCGGTGGCGTGGAGCACATGGGCCACGTCAGCATGATGCATGGCGTAGACCCCAACCCG > KT2440/2432481‑2432730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |