| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,138,367 | 0 | T | G | 52.0% | 9.2 / 25.2 | 25 | F240V (TTT→GTT) | rbsC_2 | Ribose import permease protein RbsC |
| Reads supporting (aligned to +/- strand): ref base T (3/9); new base G (13/0); total (16/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.30e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGC‑TTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGG > KT2440/3138238‑3138499 | acGCCATTCGGCCTGGGTCTGTCCGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGtt < 6:167279/136‑1 (MQ=255)acGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACTTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTgttgtt > 4:36088/1‑136 (MQ=255)acGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGtt > 8:108079/1‑136 (MQ=255) aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTgggcg > 2:245934/1‑136 (MQ=255) gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCTg > 7:62145/1‑136 (MQ=255) gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTGTTgggggggcgg > 5:109872/1‑132 (MQ=255) gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGAGCAGCGGTCTGTGCGGCCGCCTGGCCGCGGGTTGTGgggcgggggg > 6:38244/1‑132 (MQ=255) gCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTTTTGGGGGGGCTGGtt > 2:64391/1‑135 (MQ=255) gCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTGTTGTTCGGCGGGCTGGTTGAGCGggagca > 7:180836/1‑136 (MQ=255) cGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTGAGCGGCAGCaccacc > 4:162169/1‑136 (MQ=255) cGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTGTGGGGGCTGGTTGAGAGGCGGCcccacc > 1:91965/1‑136 (MQ=255) tACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCg < 7:64087/136‑1 (MQ=255) acagggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTGCAGCGGCAGCACCACCGGTTCCGGCGAGAt < 1:174208/132‑1 (MQ=255) aGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAt < 8:219568/136‑1 (MQ=255) ggcggcTAATGCCGCTGGCCTGTCGGTGCGGGGCGTCGTGTTCGCCAGCTACCTGATCAGCGGGCTGGGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtg < 3:139654/136‑1 (MQ=255) gCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTTTTGGGGGGGCGGGGGCAGCGGCCGCCCCCCCGGGTCCCGCGGGCTGCTGGGGTCCGagggggg > 2:209766/1‑135 (MQ=255) tCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGGCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCAt < 8:199764/134‑1 (MQ=255) tACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTGGTCGGCGGCCGGGTTCAGCGGCCGCACCCCCGGGTGCGGCGAGCTGCTGCTGTGCCGGGGGGGCATCGC‑GTTTATTgtgg > 8:11173/1‑136 (MQ=255) aCGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCGGGGTGAGCGGCCGCCCCACCCGGTGCGGCGCGCTGCTGCGGTGCGGGGTGGGCCTCGG‑GGTTGTTggggg > 3:235214/1‑135 (MQ=255) tCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTTGGCGGCCGGGGTCAGCCGCCCCCCCCCCCGGTGCGGCGCGCTGCTGCCGTGCGTTGTGGGCAGCGCGATTTCTTgggggtatgt > 2:233158/1‑130 (MQ=255) aTCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGC‑TTTTCTTGGGGTGATCTTCTCCCAGCGTCTGc < 4:141051/136‑1 (MQ=255) aGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCGGGTTGAGCGGCAGCCCCACCGGTTGCGGCGAGATGCTGCTGTGCGTGGTGGCCAACGC‑GTTTGTTGGGGTGTTATTATACCAGCGTCTGCAGc > 4:84325/1‑136 (MQ=255) tgtgCGGCAGCCTGGCCGCGGTTTTTTCGGCGGGCTGGGTCAGCGGCAGCACCACCCGGTGCGGCGAGATGCTGCTGTGCGTGGTGGGCCTCGC‑GTTTAGTGGGGTGCTCTTCTACCAGCGTCTGCCGCCGCGCaa > 4:115856/1‑135 (MQ=255) cccGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCAGCGGTTCGGGCGAGATGCTGCTGTCCGTTGTGGCCATCGA‑TTTTCTTGGTGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACgct < 5:244433/133‑1 (MQ=255) gcgTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGC‑TTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTgg < 1:245934/136‑1 (MQ=255) | ACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGC‑TTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGG > KT2440/3138238‑3138499 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |