Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 495,400 A→G V213V (GTA→GTG)  CJ019_00444 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440495,4000AG100.0% 154.7 / NA 41V213V (GTA→GTG) CJ019_00444hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/17);  total (24/17)

GCCTCGGTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTACTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTGGATCGTGCTGCT  >  KT2440/495272‑495528
                                                                                                                                |                                                                                                                                
gCCTCGGTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGcc                                                                                                                            >  3:141885/1‑135 (MQ=255)
gCCTCGGTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCt                                                                                                                           <  5:216680/136‑1 (MQ=255)
gCCTCGGTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAACGCCACCCCGGACCCGGCCATTTACCTGGACCTTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGcc                                                                                                                            >  1:144148/1‑135 (MQ=255)
     ggTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACGAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTAcc                                                                                                                        <  8:6264/134‑1 (MQ=255)
                     gATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTgg                                                                                                      >  5:183835/1‑136 (MQ=255)
                                  aGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGa                                                                                          <  3:187467/135‑1 (MQ=255)
                                       gCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGc                                                                                    >  6:151528/1‑136 (MQ=255)
                                          aaGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCaga                                                                                 <  3:9196/136‑1 (MQ=255)
                                          aaGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCa                                                                                   >  2:213939/1‑134 (MQ=255)
                                            ggCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGGGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGc                                                                               <  4:38171/136‑1 (MQ=255)
                                               cACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAAc                                                                            >  3:98247/1‑136 (MQ=255)
                                                    cGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACctgct                                                                       <  2:158756/136‑1 (MQ=255)
                                                    cGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACctgct                                                                       >  6:114242/1‑136 (MQ=255)
                                                    cGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTAATTGTTGGCCAACCTGCCGACGCGGCAGAGCAACctgct                                                                       <  2:144148/136‑1 (MQ=255)
                                                      gACCAGGCCATTTACCTTGAGATTTTTGTTCGTACCCTGTGGATGGGCGGGGTGATTACCTCTATCTGGCTGGTGCTGGCCTAGCCTCTGGACTACTTGTTGGCCAAGCTGCCGACCCGGCAGAGCAACCTGCtga                                                                     <  2:250629/136‑1 (MQ=255)
                                                               aTTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTgg                                                            >  5:87290/1‑136 (MQ=255)
                                                                    ccTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCTCTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTg                                                       <  1:205588/136‑1 (MQ=255)
                                                                    ccTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTg                                                       <  5:158351/136‑1 (MQ=255)
                                                                           aTTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCt                                                >  1:199666/1‑136 (MQ=255)
                                                                                  tCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGa                                          <  5:114242/135‑1 (MQ=255)
                                                                                      tACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCg                                     <  8:217375/136‑1 (MQ=255)
                                                                                       aCCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGa                                    >  7:75452/1‑136 (MQ=255)
                                                                                               gATGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTa                            >  4:225566/1‑136 (MQ=255)
                                                                                                aTGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTAc                           >  4:191712/1‑136 (MQ=255)
                                                                                                aTGGGCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTAGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCGTGGTAc                           >  8:137649/1‑136 (MQ=255)
                                                                                                   ggCGTGGTGATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGa                         <  6:89691/135‑1 (MQ=255)
                                                                                                      gtggtgATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACa                                        >  3:66861/1‑117 (MQ=255)
                                                                                                      gtggtgATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACa                                        <  4:66861/117‑1 (MQ=255)
                                                                                                      gtggtgATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCGTGGTACGAGTgg                     >  3:131042/1‑136 (MQ=255)
                                                                                                      gtggtgATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTgg                     >  5:51219/1‑136 (MQ=255)
                                                                                                      gtggtgATTACCACTATCTGCCTGGTGCTGGCCTACACACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTgg                     >  5:36444/1‑136 (MQ=255)
                                                                                                        ggtgATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTggcg                   >  4:58411/1‑136 (MQ=255)
                                                                                                        ggtgATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTggcg                   >  1:182529/1‑136 (MQ=255)
                                                                                                         gtgATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTggcg                   <  1:133037/135‑1 (MQ=255)
                                                                                                          tgATTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTggcggc                 >  3:54134/1‑136 (MQ=255)
                                                                                                            aTTACCACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGCTCCGGGTACGAGTGGCGGCGt               >  1:120860/1‑136 (MQ=255)
                                                                                                                ccACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACGTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTGGa            <  3:191712/135‑1 (MQ=255)
                                                                                                                 cACTATCTGCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTGGATc          >  2:55042/1‑136 (MQ=255)
                                                                                                                         gCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTg                                                    <  3:95745/86‑1 (MQ=255)
                                                                                                                         gCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTg                                                    >  4:95745/1‑86 (MQ=255)
                                                                                                                         gCCTGGTGCTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTGGATCGtgctgct  >  8:118240/1‑136 (MQ=255)
                                                                                                                                |                                                                                                                                
GCCTCGGTGGACCATCGCATCGATGACCTTGGTGAGCTAGCCAAGGCCACCCCGGACCAGGCCATTTACCTGGACATTTTTGTCCGTACCCTGTGGATGGGCGTGGTGATTACCACTATCTGCCTGGTACTGGCCTACCCACTGGCCTACTTGTTGGCCAACCTGCCGACCCGGCAGAGCAACCTGCTGATGATTCTGGTGCTGTTGCCCTTCTGGACATCGATCCTGGTACGAGTGGCGGCGTGGATCGTGCTGCT  >  KT2440/495272‑495528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑