Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	4,257,309	C→T	intergenic (‑238/‑44)	CJ019_03991 ← / → tsaM1	putative ABC transporter ATP‑binding protein/Toluene‑4‑sulfonate monooxygenase systemiron‑sulfur subunit TsaM1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,257,309	0	C	T	100.0%	121.9 / NA	35	intergenic (‑238/‑44)	CJ019_03991/tsaM1	putative ABC transporter ATP‑binding protein/Toluene‑4‑sulfonate monooxygenase systemiron‑sulfur subunit TsaM1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/17);  total (18/17)

ACGTGCCGCTGCAGCCGCCCCCTCGAATCGCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTGGGCCGGCAGATCTGCGGGGAAA  >  KT2440/4257181‑4257437                                                                                                                                 |                                                                                                                                 acgTGCCGCTGCAGCCGCCCCCTCGAATCGCAGAGGGCGCCCCATCAATGAATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACATACCACGCCGACCGCAGCTTTAATg                                                                                                                            >  3:204875/1‑135 (MQ=255)   gTGCCGCTGCAGCCGCCCCCTCGAATCGCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCAGCACCTACCGAACAAAATGCATTGACCTAACACGCCGACCGCAGCTTTAATGGAt                                                                                                                         >  4:173772/1‑136 (MQ=255)    tGCCGCTGCAGCCGCCCCCTCGAATCGCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATc                                                                                                                        <  2:150446/136‑1 (MQ=255)                           aTCGCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCgg                                                                                                 <  6:42005/136‑1 (MQ=255)                              gCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGgag                                                                                               <  5:129724/135‑1 (MQ=255)                              gCAGAGGGCGCCCCATCAATGCATCCACTAAAAACCGATAATCGAACAGAATCCGCACCTACCGAAAAAAATGCATTGACCAACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGgaga                                                                                              <  1:99771/136‑1 (MQ=255)                               cAGAGGGCGCCCCATCAATGGATCCACTAAAACCCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCATCTTTAATGGATCCATTAAATAAAAACAAAACCCGGagag                                                                                             >  8:55438/1‑136 (MQ=255)                                  agGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCCGCTTTAATGGATCCATTAAATAAAAACAAACCCCGGAGAGCCt                                                                                          >  3:11955/1‑136 (MQ=255)                                     gcgcCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTccc                                                                                       <  4:123013/136‑1 (MQ=255)                                                        aCTAAAAACCGATAATAGCACAGAATCCGCACCTACAGAACAAAATGCATTGACCTACCACGCCGACAGCAGCTAGAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCACAAAAacac                                                                      >  5:188721/1‑134 (MQ=255)                                                        aCTAAAAACAGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCt                                                                    >  3:189440/1‑136 (MQ=255)                                                         cTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTg                                                                   >  2:5753/1‑136 (MQ=255)                                                          tAAAAACCTATAATCGCACACAAGCCGCACCTACCCAAAAAATTGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTgg                                                                  <  2:104233/136‑1 (MQ=255)                                                           aaaaaCCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTgg                                                                  >  3:1610/1‑135 (MQ=255)                                                            aaaaCCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTa                                                                >  2:47794/1‑136 (MQ=255)                                                                    tattagCACAGAATCCGACCGTCCCGAACAATATGGATTGAACTACCACCCCGACCGCAGCTTTATTGGATCTATTAATTATAATCAATACCCGGAGAGGCTCCCATGTACCCCAAAAACACCTGGTACGTCGc                                                          <  2:181412/129‑1 (MQ=255)                                                                      aTCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTg                                                       >  1:104233/1‑135 (MQ=255)                                                                            caGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAACACCCGGAGAGCCTCCCAAGTACCCCAAAAACACCTGGTACGTCGCCTGCAccccc                                                <  1:201233/136‑1 (MQ=255)                                                                              gAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAACAACACCTGGTACGTCGCCTGCACCCCCGa                                              >  8:20280/1‑136 (MQ=255)                                                                              gAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCt                                                        >  1:58060/1‑126 (MQ=255)                                                                              gAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCt                                                        <  2:58060/126‑1 (MQ=255)                                                                                 tCCGCACCTAACGAACAAAATGCATTGACCTACCACGCAGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATga                                           <  4:180445/136‑1 (MQ=255)                                                                                           ccGAACAAAATGCATTGACCTACCCCGCCGACCGCAGCTTTAATGGATCGATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGccac                                  <  4:222114/135‑1 (MQ=255)                                                                                            cGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGgag                                                                                               <  4:113087/73‑1 (MQ=255)                                                                                            cGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGgag                                                                                               >  3:113087/1‑73 (MQ=255)                                                                                            cGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGccacc                                 >  4:244755/1‑135 (MQ=255)                                                                                                cAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAAcc                            >  1:210392/1‑136 (MQ=255)                                                                                                   aaTGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCt                         >  2:6423/1‑136 (MQ=255)                                                                                                    aTGCATTGACCTACAACTCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCATAAACTCCTGGTACGTCGCCTGCACGCCCGATGAGATCGCCACCAAACCCCTg                        <  7:242570/136‑1 (MQ=255)                                                                                                       cATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTGGGc                     <  1:47794/136‑1 (MQ=255)                                                                                                                    cGCCGACCGCAGCTTTAATGGAACCATTAAATAAAAACAAAACACGCAGAGCCTCCCATGTAGCCCAAAAACACCAGCTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTCCGCCGGTAGATCAGCg        >  4:127808/1‑136 (MQ=255)                                                                                                                       cGACCGCAGCTTTAATGGATCCATTAAATAAAAACACAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTGGGCCGGCAGATCTGCgggg     <  8:128961/136‑1 (MQ=255)                                                                                                                         aCCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGagag                                                                                             <  7:157863/46‑1 (MQ=255)                                                                                                                         aCCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGagag                                                                                             >  8:157863/1‑46 (MQ=255)                                                                                                                          ccGCAGCTTTAATGGATCAATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTGGGCCGGCAGATCTGCGGGGaaa  <  6:158715/136‑1 (MQ=255)                                                                                                                                 |                                                                                                                                 ACGTGCCGCTGCAGCCGCCCCCTCGAATCGCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTGGGCCGGCAGATCTGCGGGGAAA  >  KT2440/4257181‑4257437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑