| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | KT2440 | 4,425,093 | G→A | V38V (GTG→GTA) | fabG_6 → | 3‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,425,093 | 0 | G | A | 95.7% | 70.0 / ‑3.8 | 23 | V38V (GTG→GTA) | fabG_6 | 3‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (13/9); total (14/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
ATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGG > KT2440/4424963‑4425211 | aTAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTAATCGt > 7:162286/1‑136 (MQ=255)aTAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTAATCGt > 3:54549/1‑136 (MQ=255)aTAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCAAGAAATCGt > 1:193762/1‑136 (MQ=255) aaGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTAATCGTTTCCa < 6:241219/136‑1 (MQ=255) aGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTAATCGTTTCCAg < 8:94464/136‑1 (MQ=255) aGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTAATCGTTTCCAg < 3:112121/136‑1 (MQ=255) aTGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTAATCGTTTCCAGCCGCAAGCtt > 8:35388/1‑135 (MQ=255) tggccAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTAATCGTTTCCAGCCGCAAGCTTGa < 1:75740/133‑1 (MQ=255) cccACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCCTCGCCCACTTGCTCGCGCAGCAAGGGGCCCCTGTAATCGTTTACAGCCGCAAGCTTGCCGGGTGc > 5:20487/1‑134 (MQ=255) cACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTAATCGTTTCCAGCCGCAAGCTTGACGGGTGCcag > 8:83614/1‑135 (MQ=255) ttCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTAATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGt < 4:193770/134‑1 (MQ=255) gACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTAATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGcc < 5:57046/135‑1 (MQ=255) ccTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTAATCGTTTCCAGCCGCAAGCTTGCCGGGTGCCAGCAGGTGGCCGAc > 5:15149/1‑136 (MQ=255) ccTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTAATCGTTTCCAGCCGCAAGCTTGACGGGTGCGAGCAGGTGGCCGAc > 3:175969/1‑136 (MQ=255) aCGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCcat > 8:184369/1‑136 (MQ=255) cGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTAATCGTTTCCAGCCGCAAGCGTGACGGGTGCCAGCAGGTGGCCGACGCcat > 4:55279/1‑135 (MQ=255) ggCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTAATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCcatca < 8:126208/136‑1 (MQ=255) cTTGGTTCCCGGCGCCAGCCGTGCCATCCGCCAGGCCATCGCCCACTGGCCCCCCCCCCACGGGGCCCATCTAATCGTTACCAGCCCCAACCTTCCCGGTTGCCCGCCTGTGGCCCACGCCATCATTgccgcctcc < 4:233428/136‑4 (MQ=255) cgggCGCCCGCCGGGGCATCGGCGAGCCCAGCGCCCACTTGCTCGCCCACCAGGGGGCCCAGGTATTCGTTCCCCCCCGCACGCTTGCGGGGGGCCAGCAGGGGGCCGACGCCATAATTGCCGCCGGCGGCAAgg < 8:222358/133‑1 (MQ=255) gAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTAATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCCCATTg > 2:21238/1‑136 (MQ=255) gcagcaAGGGGCCCATGTAATCGTTTCCAGCAGCAAGCTTGACGGGTGCCAGAAGGTGGCCGACGCCATCATTGCCGCCGGCGGCACGGCCACGGCTGTGGCCTGCGACATTGGTGAGCTGGAACAGCTTcagcag > 8:30937/1‑136 (MQ=255) cagcaAGGGGCCCATGTAATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAgg > 1:89414/1‑136 (MQ=255) cagcaAGGGGCCCATGTAATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCAGCCACATTGGTGAGCTGGAACAGATTCAGCAgg > 5:205366/1‑136 (MQ=255) | ATAACAAGGAAAACAGCATGTCCAAGACCCACCTGTTCGACCTCGACGGCAAGATTGCCTTTGTTTCCGGCGCCAGCCGTGGCATCGGCGAGGCCATCGCCCACTTGCTCGCGCAGCAAGGGGCCCATGTGATCGTTTCCAGCCGCAAGCTTGACGGGTGCCAGCAGGTGGCCGACGCCATCATTGCCGCCGGCGGCAAGGCCACGGCTGTGGCCTGCCACATTGGTGAGCTGGAACAGATTCAGCAGG > KT2440/4424963‑4425211 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |