| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,055,964 | Δ1 bp | 7.3% | coding (479/762 nt) | CJ019_04749 → | IS630 family transposase ISPpu1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,055,964 | 0 | G | . | 7.3% | 182.2 / 7.8 | 55 | coding (479/762 nt) | CJ019_04749 | IS630 family transposase ISPpu1 |
| Reads supporting (aligned to +/- strand): ref base G (27/24); new base . (2/2); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01 | |||||||||||
GCATCGGCATCAGGAGTGGCTGGCCTTCCTGAAAAAGATCAACCGAGAAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGACGGCAGCTTCAGTTCCACACGTGAACTGGCCAGCT > KT2440/5055833‑5056095 | gCATCGGCATCAGGAGTGGCTGGCCTTCCTGAAAAAGATCAACCGAGAAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCa < 3:166286/135‑1 (MQ=255)gCATCGGCATCAGGAGTGGCTGGCCTTCCTGAAAAAGATCAACCGAGAAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCa < 6:222151/135‑1 (MQ=255) aTCGGCATCAGGAGTGGCTGGCCTTCCTGAAAAAGATCAACCGAGAAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGC‑GCATcc > 3:50283/1‑135 (MQ=255) aTCGGCATCAGGAGTGGCTGGCCTTCCTGAAAAAGATCAACCGAGAAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGC‑GCATcc > 3:46192/1‑135 (MQ=255) cATCAGGAGTGGCTGGCCTTCCTTAAAAAGATCAACCGAGAAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGa < 8:31634/135‑1 (MQ=255) cATCAGGAGTGGCTGGCCTTCCTGAAAAAGATCAACCGAGAAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGC‑GCATCCACGAt < 4:46192/135‑1 (MQ=255) cATCAGGAGTGGCTGGCCTTCCTGAAAAAGATCAACCGAGAAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGC‑GCATCCACGAt < 4:50283/135‑1 (MQ=255) cAGGAGTGGCTGGCCTTCCTGAAAAAGATCAACCGAGAAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTc < 4:191203/135‑1 (MQ=255) aGTGGCTGGCCTTCCTGAAAAAGATCAACCGAGAAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCAcc > 3:35603/1‑135 (MQ=255) ggctggcCTTCCTGAAAAAGATCAACCGAGAAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGc < 4:298776/135‑1 (MQ=255) ggcCTTCCTGAAAAAGATCAACCGAGAAACCCCCAAAGCTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCCTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCAtt > 6:263433/1‑134 (MQ=255) ccTTCCTGAAAAAGATCAACCGAGAAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCa < 6:139009/135‑1 (MQ=255) aaaaGATCAACCGAGAAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCt < 2:38040/135‑1 (MQ=255) ataGATCAACCGAGAAACCCCCAAAGGTCTGCAACTTCATTTGATCGTGCATAACTACGCCACTCACAAGCATCGCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCt < 2:42874/132‑1 (MQ=255) aTCAACCGAGAAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGc < 7:67800/135‑1 (MQ=255) gagaAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGa > 6:93675/1‑135 (MQ=255) aacggTCTGCAACTGCATTTGATCGTGCAAAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGtt < 7:269568/132‑1 (MQ=255) cAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCCAGCTCCTGGATGAACATGGTTGAGCGcttc > 3:273776/1‑135 (MQ=255) cAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGcttc > 5:96613/1‑135 (MQ=255) aaCTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGTGcttc < 6:287268/134‑1 (MQ=255) aaCTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGcttc < 1:42983/134‑1 (MQ=255) aCTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGcttctt < 6:96613/135‑1 (MQ=255) tttGATCGTGTATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGa < 5:34930/135‑1 (MQ=255) tttGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGa < 4:35603/135‑1 (MQ=255) tttGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGa < 3:111474/135‑1 (MQ=255) tttGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGa < 5:93675/135‑1 (MQ=255) ttGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGat > 7:269159/1‑135 (MQ=255) ggATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCCCCGt > 2:140771/1‑135 (MQ=255) aTAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGttt > 4:245426/1‑135 (MQ=255) aTAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGttt > 1:244651/1‑135 (MQ=255) aTAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGttt > 8:17177/1‑135 (MQ=255) aTAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGttt > 4:82304/1‑135 (MQ=255) cACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGa > 7:40136/1‑135 (MQ=255) cACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGa > 2:84062/1‑135 (MQ=255) acaAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATCAACATGGTTGAGCGCTTCGTCCTGGATATCACCGTTTACCTGCGCGACGGCa > 8:216839/1‑135 (MQ=255) caAGCATACCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCAACTGCATTTCACCCCGACCACAAGCTCCTGGATGAACATTGTTGAGCGCTTCTTCCGGGATATGACCGTTTACCTGCGCGACGTCAg > 4:229838/1‑135 (MQ=255) cccGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGAc < 5:131610/123‑1 (MQ=255) cccGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGAc < 5:126570/123‑1 (MQ=255) cccGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGAc > 6:131610/1‑123 (MQ=255) cccGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGAc > 6:126570/1‑123 (MQ=255) ccGGGGTCAAGGAATGCCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCTACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGACGGCAGCTTCAGtt < 2:154704/135‑1 (MQ=255) ccGGGGGCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGACGGCAGCTTCAGtt < 5:95781/135‑1 (MQ=255) cGTGGTCAAGGAATGGCTTAAGCGTCATCAACGATTCCACCTGCATTTCACCCCGACATCAAGCTCATGGATGAACATTGTTGAGAGCTTCTTACGGGATATCACCGTTTACCTGCGCGACGGCAGCTTCAGtt > 4:139078/1‑134 (MQ=255) cGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGACGGCAGCTTCAGtt > 6:77486/1‑134 (MQ=255) cGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGACGGCAGCTTCAGtt > 4:154945/1‑134 (MQ=255) cGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGACGGCAGCTTCAGTTc > 4:143586/1‑135 (MQ=255) gTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGACGGCAGCTTCAGTTcc > 6:229494/1‑135 (MQ=255) gTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGACGGCAGCTTCAGTTcc > 4:228540/1‑135 (MQ=255) tCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGACGGCAGCTTCAGTTCCacac > 3:116635/1‑135 (MQ=255) aaGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTGCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGACGGCAGCTTCAGTTCCACACg > 6:108685/1‑134 (MQ=255) aaTGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTgc < 5:275402/105‑1 (MQ=255) aaTGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTgc > 6:275402/1‑105 (MQ=255) ctgtcTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCGCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGACGGCAGCTTCAGTTCCACACGTGAACt < 2:125441/131‑1 (MQ=255) tGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTGCCTGCGCGACGGCAGCTTCAGTTCCACACGTGAACTg < 6:184422/135‑1 (MQ=255) aGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACAAGGATGAGCGCTTCTTCCGGGATATCACCGTTAACCACCGCGACGGCAGCTTCAGTTCCACACGTCAACTGGCCAGCt > 2:7874/1‑135 (MQ=255) | GCATCGGCATCAGGAGTGGCTGGCCTTCCTGAAAAAGATCAACCGAGAAACCCCCAAAGGTCTGCAACTGCATTTGATCGTGGATAACTACGCCACTCACAAGCATCCCGTGGTCAAGGAATGGCTTAAGCGGCATCCACGATTCCACCTGCATTTCACCCCGACCTCAAGCTCCTGGATGAACATGGTTGAGCGCTTCTTCCGGGATATCACCGTTTACCTGCGCGACGGCAGCTTCAGTTCCACACGTGAACTGGCCAGCT > KT2440/5055833‑5056095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |