| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 601,357 | C→T | 10.5% | P60S (CCT→TCT) | btuF → | Vitamin B12‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 601,357 | 0 | C | T | 10.5% | 111.9 / 4.5 | 38 | P60S (CCT→TCT) | btuF | Vitamin B12‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (18/16); new base T (2/2); total (20/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGCCAGGCAGTGTGCCGCCGGCCCAGCGCGACCAACTCAAGCGCCTGGGTATCGCC > KT2440/601226‑601485 | tGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCtt > 8:101539/1‑135 (MQ=255)tGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCtt > 4:181394/1‑135 (MQ=255)tGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCtt > 7:148751/1‑135 (MQ=255)tGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCtt > 8:65097/1‑135 (MQ=255) gAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCggg > 2:34583/1‑135 (MQ=255) gAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCggg > 4:117740/1‑135 (MQ=255) gAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCggg > 1:210073/1‑135 (MQ=255) aCCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGgcg > 6:171603/1‑135 (MQ=255) cGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGCCCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAgg > 8:89978/1‑134 (MQ=255) gtgtCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGc < 2:166841/135‑1 (MQ=255) gtgtCGGCAGGCGGGCCCCATCTATGAGCGAGATCATGCTTGAGCTGAAGGCCGACGACCTGCTGGTGGGCGGGCTCTATGGCGGAGAACGGCCGGCTGCACTGCGCCACTTGTCTTCGGTCGGGCGCCAAGGGc < 8:246939/135‑1 (MQ=255) gTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGTCTTCGGTCGGGCGCCAAGGGc < 8:242896/126‑1 (MQ=255) gTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGTCTTCGGTCGGGCGCCAAGGGc > 7:242896/1‑126 (MQ=255) gTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGTCTTCGGTCGGGCGCCAAGGGCAACTGGACa > 7:246939/1‑135 (MQ=255) cccccTCGATGAGGGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGa < 4:263947/133‑1 (MQ=255) cccccTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGa < 1:34583/133‑1 (MQ=255) ccTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGcc > 1:213081/1‑135 (MQ=255) tGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCa < 2:187247/135‑1 (MQ=255) cATGCTGGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGccc < 4:155947/135‑1 (MQ=255) tGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGcccc < 7:101539/134‑1 (MQ=255) tGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCg < 6:288010/135‑1 (MQ=255) tGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTtgc > 2:134315/1‑135 (MQ=255) gcggCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTtgctg < 6:57167/133‑1 (MQ=255) gCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGctgctg > 1:273823/1‑135 (MQ=255) cgacCTGCTGGTGGGCGTGCGCGAGGGCGGCGAACGGCCGGCGGCACTGCGCCACTTGCCTTCGCTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCGGCGCCCCGACCTGTTGCTGCTGTGGcc < 4:9008/135‑1 (MQ=255) ccTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGCCAg > 8:152623/1‑134 (MQ=255) tggtggGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGCCAGGCAgtg > 7:269105/1‑135 (MQ=255) gCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGCCAGGCAGTGTGCCGCCGGc > 6:83627/1‑135 (MQ=255) tCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCACCTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCGGTGGCCAGGCAGTGTGCCGCCGGc > 7:259090/1‑133 (MQ=255) gcgctGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGGCGGGCGCCAATGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCGGTGGCCAGGCAGTGTGCCGCCGGccc < 3:211833/131‑1 (MQ=255) gcGATGGCGGCGAACGGCCGGCGGCACTGCGCCACTTGCCTGCGGGCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGCCAGGCAGTGTGCCGCCGGccc < 7:291551/134‑1 (MQ=255) ggcggcGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGCCAGGCAGTGTGCCGCCGGCCCAgcgc < 2:210073/135‑1 (MQ=255) ggcggcGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGGCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGCCAGGCAGTGTGCCGCCGGCCCAgcgc < 3:117740/135‑1 (MQ=255) gAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGCCAGGCAGTGTGCCGCCGGCCCAGCGCGACCaa < 2:176169/135‑1 (MQ=255) aaCGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGCCAGGCAGTGTGCCGCCGGCCCAGCGCGACCAAc > 1:290398/1‑135 (MQ=255) cACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGCCAGACAGTGTGCCGCCGGCCCAGCGCGACCAACTCAAGCGCCTgg < 2:213081/135‑1 (MQ=255) cgcCACTGGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTGGCTGCTGTGGCCAGGCGGTGTGCCGCCGGCCCAGCGCGACCAACTCAAGCGCCTGGGTATc < 7:27710/135‑1 (MQ=255) aaCTTGCCTTCGGTAGGGCGCCAAGGGCAACAGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGCCAGGCAGTGTGCAGCCGGCCCAGAGCGACCAACTCAAGCGCCTGGGTATCGcc > 4:246834/2‑135 (MQ=255) | TGGCTGCAGAACCGCTACGTGTCGTCAGTCTGGCCCCCTCGATGAGCGAGATCATGCTTGAGCTGCAGGCCGACGACCTGCTGGTGGGCGTGCTCGATGGCGGCGAACGGCCGGCTGCACTGCGCCACTTGCCTTCGGTCGGGCGCCAAGGGCAACTGGACATGGAGCGCCTGCTCAGCCTGCGCCCCGACCTGTTGCTGCTGTGGCCAGGCAGTGTGCCGCCGGCCCAGCGCGACCAACTCAAGCGCCTGGGTATCGCC > KT2440/601226‑601485 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |