| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 620,951 | G→A | 7.8% | intergenic (‑33/+75) | CJ019_00589 ← / ← eutC | hypothetical protein/Ethanolamine ammonia‑lyase light chain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 620,951 | 0 | G | A | 7.8% | 160.0 / 3.8 | 51 | intergenic (‑33/+75) | CJ019_00589/eutC | hypothetical protein/Ethanolamine ammonia‑lyase light chain |
| Reads supporting (aligned to +/- strand): ref base G (21/26); new base A (2/2); total (23/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01 | |||||||||||
CGGGGTAGGGCAGTTGCCCGTAGAACTCACGGTATTTGACGAACAACGGGGTGAGCAGGTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTACCTTTCTGGTTGGCGCTGTCTTCATTCTCCAGG > KT2440/620825‑621083 | cGGGGTAGGGCAGTTGCCCGTAGAACTCACGGTATTTGACGAACAACGGGGTGAGCAGGTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCca < 8:291995/135‑1 (MQ=255)cGGGGTAGGGCAGTTGCCCGTAGAACTCACGGTATTTGACGAACAACGGGGTGAGCAGGTCGAGGTGTTCCAGGGTTGCTTGGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCca < 8:290072/135‑1 (MQ=255) gaGTTGCCAGTAGAACTCACGGTATTTGACGAACAACGGTGTGAGCAGGTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCa < 1:51670/134‑1 (MQ=255) gTTGCCCGTAGAACTCACGGTATTTGACGAACAACGGGGTGAGCAGGTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAg > 3:179790/1‑135 (MQ=255) cccGTAGAACTCACGGTATTTGACGAACAACGGGGTGAGCAGGTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGCGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCa > 4:84130/1‑135 (MQ=255) cccGTAGAACTCACGGTATTTGACGAACAACGGGGTGAGCAGGTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCAGAATGCCAGCAGCGTTCAAGGGCa > 7:46057/1‑135 (MQ=255) cccGTAGAACTCACGGTATTTGACGAACAACGGGGTGAGCAGGTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCAGAATGCCAGCAGCGTTCAAGGGCa > 7:41210/1‑135 (MQ=255) ccGTAGAACTCACGGTATTTGACGAACAACGGGGTGAGCAGGTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCAt < 7:119947/135‑1 (MQ=255) tAGAACTCACGGTATTTGACGAACAACGGGGTGAGCAGGTCGAGGTGGTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCAtgct < 2:72216/135‑1 (MQ=255) aaCTCACGGTAATTGACGAACTACGGGGTGAGCAGGTCGAGGTGTTCCAGTGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGcc < 2:106956/135‑1 (MQ=255) cGGTATTTGACGAACCACGGGGTGAGCAGGTCGAGGTGTTCCAGGGGTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAAt < 5:213638/133‑1 (MQ=255) cGGTATTTGACGAACAACGGGGTGAGCAGGTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAAt < 3:136108/133‑1 (MQ=255) cGGTATTTGACGAACAACGGGGTGAGCAGGTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAAt < 6:300489/133‑1 (MQ=255) cGGTATTTGACGAACAACGGGGTGAGCAGGTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAAt < 6:169312/133‑1 (MQ=255) cGGTATTTGACGAACAACGGGGTGAGCAGGTCGAGGTGTTCCAGGGGTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAAt < 2:14415/133‑1 (MQ=255) taattGACGAACCACGGGGTGAGCAGGTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACg < 5:277634/132‑1 (MQ=255) tGAGCAGGTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCt > 7:256825/1‑135 (MQ=255) cAGGTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCg > 8:281333/1‑135 (MQ=255) gTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGa < 1:242832/135‑1 (MQ=255) aGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCAGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGa > 8:295557/1‑135 (MQ=255) tgtTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGAttt < 6:261798/135‑1 (MQ=255) aGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAAt > 5:243702/1‑91 (MQ=255) aGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAAt < 6:243702/91‑1 (MQ=255) gTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCtt < 3:267405/135‑1 (MQ=255) gTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGATGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCtt < 7:206359/135‑1 (MQ=255) ttGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCGAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGa > 8:243513/1‑135 (MQ=255) ttGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGa > 3:230168/1‑135 (MQ=255) ttGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGa > 4:105875/1‑135 (MQ=255) ttGATGATGCGCATCTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGTTACTAGTGCAATCCGCCTTCCGTGACATATTTTTTACCCTATGCAGa > 4:240976/1‑135 (MQ=255) atgCGCATGTGCGGCCCTCAGTGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAg > 8:165746/1‑133 (MQ=255) atgCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAg > 4:43293/1‑133 (MQ=255) cATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCagcagc < 4:229946/49‑1 (MQ=255) cATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCagcagc > 3:229946/1‑49 (MQ=255) cATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTa > 6:11916/1‑135 (MQ=255) tgtgCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTAcc < 4:179790/135‑1 (MQ=255) gtgCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCa < 6:27890/51‑1 (MQ=255) gtgCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCa > 5:27890/1‑51 (MQ=255) gCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTACCttt > 3:149635/1‑135 (MQ=255) cTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTACCTTTCTGGtt > 8:66971/1‑135 (MQ=255) agagTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTACCTTTCTGGTTGgc > 6:240529/1‑135 (MQ=255) gagTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTACCTTTCTGGTTgg < 7:243513/133‑1 (MQ=255) aTTTGGGACACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTACCTTTCTGGTTGGCGCTGTCt < 4:140691/135‑1 (MQ=255) aTTTGGGAAACAGCAGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTACCTTTCTGGTTGGCGCTGTCt < 8:46057/135‑1 (MQ=255) aTTTGGGAAACAGCAGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTACCTTTCTGGTTGGCGCTGTCt < 8:41210/135‑1 (MQ=255) ttGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTACCTTTCTGGTTGGCGCTGTCTTc > 5:278762/1‑135 (MQ=255) tGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTACCTTTCTGGTTGGCGCTGTCTTCa > 2:161346/1‑135 (MQ=255) gAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTACCTTTCTGGTTGGCGCTGTCTTCATtc < 7:165746/135‑1 (MQ=255) gAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTACCTTTCTGGTTGGCGCTGTCTTCATtc < 1:161346/135‑1 (MQ=255) aaCAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTACCTTTCTGGTTGGCGCTGTCTTCATtct > 4:55103/1‑134 (MQ=255) gCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTAGCTTTCTGGTTGGCGCTGTCTTCATTCTCCAgg < 5:248223/135‑1 (MQ=255) gCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTACCTTTCTGGTTGGCGCTGTCTTCATTCTCCAgg < 3:65565/135‑1 (MQ=255) | CGGGGTAGGGCAGTTGCCCGTAGAACTCACGGTATTTGACGAACAACGGGGTGAGCAGGTCGAGGTGTTCCAGGGTTGCTTTGATGATGCGCATGTGCGGCCCTCAGAGTCCATTTGGGAAACAGCGGAATGCCAGCAGCGTTCAAGGGCATGCTGCCCAATGCACGGTACGAGTGCAATCCGCCTTCCGTGACAGATTTTTTACCCTTTGCCGAGCAGGAAGTTACCTTTCTGGTTGGCGCTGTCTTCATTCTCCAGG > KT2440/620825‑621083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |