Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,198,856 A→T 10.0% intergenic (‑42/+48) CJ019_01129 ← / ← CJ019_01130 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,198,8560AT10.0% 101.1 / 4.9 40intergenic (‑42/+48)CJ019_01129/CJ019_01130hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (13/23);  new base T (2/2);  total (15/25)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.37e-01

AGCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGATGTGTTCCCTGCCGGTGACGGTGTGTGCAGGGCAACCTGTGGCGGCTTGCACTCATTCGGATTGCCCGGCTGCA  >  KT2440/1198723‑1198989
                                                                                                                                     |                                                                                                                                     
agcTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTa                                                                                                                                       >  3:148749/1‑134 (MQ=255)
 gcTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTAc                                                                                                                                      >  8:53010/1‑134 (MQ=255)
 gcTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTAc                                                                                                                                      >  6:59476/1‑134 (MQ=255)
 gcTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTAc                                                                                                                                      >  6:156877/1‑134 (MQ=255)
      aGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCaa                                                                                                                                  <  7:334/133‑1 (MQ=255)
          cACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGatata                                                                                                                            <  6:42177/135‑1 (MQ=255)
            ccaggggCGACGACACGCGGGCCTCGCTCGCCACGGGCGGGGCGCCGCTAGAAGGGATCAAGAGGGGGATGTTCGCTCATATGGTTAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGatatat                                                                                                                           <  5:282208/131‑1 (MQ=255)
                   cGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGa                                                                                                                    >  1:17804/1‑134 (MQ=255)
                   cGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGAc                                                                                                                   <  5:75149/135‑1 (MQ=255)
                   cGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGAc                                                                                                                   <  2:252665/135‑1 (MQ=255)
                        acacGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCa                                                                                                                                   <  8:266048/114‑1 (MQ=255)
                        acacGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCa                                                                                                                                   >  7:266048/1‑114 (MQ=255)
                               gggcgcgCTCGCCACGGTGGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGGTCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCTAGATATATCTAAAGACAAGCGCTTCAAt                                                                                                       <  5:256411/132‑1 (MQ=255)
                                gCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCTCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATg                                                                                                      <  6:7130/135‑1 (MQ=255)
                                 ccGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGc                                                                                                     <  6:254048/135‑1 (MQ=255)
                                  cgcgCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCt                                                                                                    <  7:2177/135‑1 (MQ=255)
                                      cTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCtgatg                                                                                                <  2:216128/135‑1 (MQ=255)
                                      cTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCtgatg                                                                                                <  7:20691/135‑1 (MQ=255)
                                      cTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCtgatg                                                                                                <  3:164219/135‑1 (MQ=255)
                                             cgggcgggGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTatca                                                                                         >  3:287004/1‑135 (MQ=255)
                                                   gggcgCGGCTCGAAGGGTTCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATAAATCTAAAGACGAGCCCTTCAATGCTGATGATTATCATCTACt                                                                                   <  2:131831/135‑1 (MQ=255)
                                                      cgccgcTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTTCTCAAGATATATCTAAAGACAAGCCCTTCAATGCt                                                                                                    <  8:39536/115‑1 (MQ=255)
                                                      cgccgcTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTTCTCAAGATATATCTAAAGACAAGCCCTTCAATGCt                                                                                                    <  8:39535/115‑1 (MQ=255)
                                                      cgccgcTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTTCTCAAGATATATCTAAAGACAAGCCCTTCAATGCt                                                                                                    >  7:39536/1‑115 (MQ=255)
                                                      cgccgcTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTTCTCAAGATATATCTAAAGACAAGCCCTTCAATGCt                                                                                                    >  7:39535/1‑115 (MQ=255)
                                                            tCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGAtg                                                                          >  4:101989/1‑135 (MQ=255)
                                                                         gAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGATGTGTTCCCTGCCgg                                                             >  7:46381/1‑135 (MQ=255)
                                                                             gggATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGATGTGTTCCCTGCCGGTGAc                                                         <  5:156877/135‑1 (MQ=255)
                                                                             gggATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGATGTGTTCCCTGCCGGTGAc                                                         <  7:53010/135‑1 (MQ=255)
                                                                                 tgctCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGATGTGTTCCCTGCCGGTGACGgtg                                                     <  3:62710/132‑1 (MQ=255)
                                                                                                          gtcgtTTGCGTTATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGATGTGTTCCCTGCCGGTGACGGTGTGTGCAGGGCAACCTGTGGCGGCtt                            <  2:72527/135‑1 (MQ=255)
                                                                                                           tcgtTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGATGTGTTCCCTGCCGGTGACGGTGTGTGCAGGGCAACCTGTGGCGGCTTg                           >  3:292828/1‑135 (MQ=255)
                                                                                                            cgtTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTc                                                                              <  5:145508/83‑1 (MQ=255)
                                                                                                            cgtTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGATGTGTTCCCTGCCGGTGACGGTGTGTGCAGGGCAACCTGTGGCGGCtt                            >  4:278780/1‑133 (MQ=255)
                                                                                                            cgtTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGATGTGTTCCCTGCCGGTGACGGTGTGTGCAGGGCAACCTGTGGCGGCTTGc                          >  7:224934/1‑135 (MQ=255)
                                                                                                            cgtTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGATGTGTTCCCTGCCGGTGACGGTGTGTGCAGGGCAACCTGTGGCGGCTTGc                          >  5:110531/1‑135 (MQ=255)
                                                                                                            cgtTTGCGATATATCGTAAGATATTACTCAAGATATATCAAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTc                                                                              >  6:145508/1‑83 (MQ=255)
                                                                                                                          cGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGATGTGTTCCCTGCCGGTGACGGTGTGTGCAGGGCAACCTGTGGCGGCTTGCACTCATTCGGAtt             <  8:37482/134‑1 (MQ=255)
                                                                                                                               gATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGATGTGTTCCCTGCCGGTGACGGTGTGTGCAGGGCAACCTGTGGCGGCTTGCACTCATTCGGATTGCCCg        <  4:260626/134‑1 (MQ=255)
                                                                                                                               gATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGATGTGTTCCCTGCCGGTGACGGTGTGTGCAGGGCAACCTGTGGCGGCTTGCACTCATTCGGATTGCCCg        <  4:287004/134‑1 (MQ=255)
                                                                                                                                    tACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGATGTGTTCCCTGCCGGTGACGGTGTGTGCAGGGCAACCTGTGGCGGCTTGCACTCATTCGGATTGCCCGGCTGCa  <  4:148749/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
AGCTTGAGGTCACCCGGGGCGAAGACACGCGGGCCGCGCTCGCCACGGGCGGGGCGCCGCTCGAAGGGATCATGAGGGGGATGTTCGCGCATAGGGATAGGGCTCCGTCGTTTGCGATATATCGTAAGATATTACTCAAGATATATCTAAAGACAAGCCCTTCAATGCTGATGATTATCATCTACTGCGTCGATGTGTTCCCTGCCGGTGACGGTGTGTGCAGGGCAACCTGTGGCGGCTTGCACTCATTCGGATTGCCCGGCTGCA  >  KT2440/1198723‑1198989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑