Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,327,427 C→T 9.5% R234W (CGG→TGG)  CJ019_01244 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,327,4270CT 9.5% 122.5 / 4.2 42R234W (CGG→TGG) CJ019_01244hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (17/21);  new base T (2/2);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

GTGGCATGGCTGTGGCAAGCGTCGTGGCCTTCATCCTGTGCAAGCGCTTTCCTTCATGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGTTGAGCCCGGCTGCACTGTCCTACGGTTCTAT  >  KT2440/1327293‑1327551
                                                                                                                                      |                                                                                                                            
gTGGCATGGCTGTGGCAAGCGTCGTGGCCTTCATCCTGTGCAAGCGCTTTCCTTCATGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTgc                                                                                                                              <  1:173612/135‑1 (MQ=255)
   gCATGGCTGTGGCAAGCGTCGTGGCCTTCATCCTGTGCAAGCGCTTTCCTTCATGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTgcggc                                                                                                                           >  8:227960/1‑135 (MQ=255)
    cATGGCTGTGGCAAGCGTCGTGGCCTTCATCCTGTGCAAGCGCTTTGCTTCATGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGGCGGTTGTCAATCAGCCTGCCTACGGTGTGGTCTGGGCGTTggggcg                                                                                                                          >  5:143491/1‑135 (MQ=255)
            tgGCAAGCGTCGTGGCCGTCATCCTGTGCCACCGCGTTACTGCATGGGTACGCCAATGCGGTGCACCCACCCTCGTGGCGCCGGTTGTCAATAAGGCTGCCTAAGGGGTTGTCTTGCCATTGCGGCGAGTTCTgt                                                                                                                  >  6:187972/1‑134 (MQ=255)
               cAAGCGTCGTGGCCTTCATCCTGTGCAAGCGCTTTCCTTCATGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGCCTTTGCGGCGCGTTGTGGCCg                                                                                                               >  1:260686/1‑135 (MQ=255)
                      cgtGGCCTTCATCCTGTGCAAGCGCTTTCCTTCATGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAg                                                                                                        >  8:217255/1‑135 (MQ=255)
                           ccTTCATCCTGTGCAAGCGCATTCCGTCATGTGTACGCCAAATCCGTGAACCCACCGAAGTGGCCCCCGTTGTCAATCATCCTGACTAACGTGTGGTCTGGACTTTTCGGCGCGTTGAGGCCGACATCAGCGAAg                                                                                                   >  4:293970/1‑135 (MQ=255)
                                       gCAAGCGCTTTCCTTCATGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTg                                                                                       <  7:227960/135‑1 (MQ=255)
                                       gCAAGCGCTTTCCTTCATGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTg                                                                                       <  1:96581/135‑1 (MQ=255)
                                         aaGCGCTTTCCTTCATGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTgg                                                                                      >  7:157494/1‑134 (MQ=255)
                                         aaGCGCTTTCCTTCATGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTgg                                                                                      >  3:76308/1‑134 (MQ=255)
                                         aaGAGCTTTCCTTCATGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATAAGCCTGACTACCGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGTCTTCAGCGAAGCCCCAATCTTTgg                                                                                      >  5:108799/1‑134 (MQ=255)
                                              cTTTCCTTCATGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGa                                                                                >  4:126211/1‑135 (MQ=255)
                                                   cTTCATGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTg                                                                            <  7:217255/134‑1 (MQ=255)
                                                       aTGGGTACGCCAATGCGGTGAACCCACCGTCGGGGCGCCGGGTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAg                                                                       <  7:126857/135‑1 (MQ=255)
                                                        tGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAg                                                                       <  3:125740/134‑1 (MQ=255)
                                                        tGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGACCAg                                                                       <  8:211161/134‑1 (MQ=255)
                                                           gTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTc                                                                   <  1:1435/135‑1 (MQ=255)
                                                                       ggTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTg                                                       <  8:157494/135‑1 (MQ=255)
                                                                            aCCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGCCTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCCGCCTCGGGCTATTGCTGGgtgt                                                  >  4:117503/1‑135 (MQ=255)
                                                                            aCCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGTGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGgtgt                                                  >  7:176372/1‑135 (MQ=255)
                                                                            aCCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGTGGCGCGCTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGgtgt                                                  >  3:108216/1‑135 (MQ=255)
                                                                            aCCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGgtgt                                                  >  1:14010/1‑135 (MQ=255)
                                                                            aCCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGgtgt                                                  <  2:61036/135‑1 (MQ=255)
                                                                                   gtcgtGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAg                                                                       <  1:129430/107‑1 (MQ=255)
                                                                                   gtcgtGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAg                                                                       >  2:129430/1‑107 (MQ=255)
                                                                                   gtcgtGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTg                                           >  3:248722/1‑135 (MQ=255)
                                                                                    tcgtGTCGCCGGTGGTCACTCAGCCTGACTAGGGTGTGGGCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTgg                                          <  5:187972/135‑1 (MQ=255)
                                                                                     cgtGGCGCCGGTTGTCAATCAGCCTGTCTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGc                                         <  6:108799/135‑1 (MQ=255)
                                                                                      gtGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCCGCCTCGGGCTATTGCTGGGTGTGTTGCTGGc                                         >  4:244473/1‑134 (MQ=255)
                                                                                              ggTTGGCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGtt                                <  3:167971/135‑1 (MQ=255)
                                                                                                        cAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGTTGAGCCCGGCt                      <  4:248722/135‑1 (MQ=255)
                                                                                                        cAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGTTGAGCCCGGCt                      <  3:49341/135‑1 (MQ=255)
                                                                                                        cAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGTTGAGCCCGGCt                      <  8:82983/135‑1 (MQ=255)
                                                                                                        cAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGTTGAGCCCGGCt                      <  6:102361/135‑1 (MQ=255)
                                                                                                        cAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGTTGAGCCCGGCt                      <  2:14010/135‑1 (MQ=255)
                                                                                                        cAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGTTGAGCCCGGCt                      <  1:21558/135‑1 (MQ=255)
                                                                                                        cAGCCTGACTACGGTGTGGGCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGTTGAGCCCGGCt                      <  6:59668/135‑1 (MQ=255)
                                                                                                         atccTGACTAGGGTGGGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGTTGAGCCCGGCt                      <  5:199105/132‑1 (MQ=255)
                                                                                                              gACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGTTGAGCCCGGCTGCACTg                >  6:108095/1‑135 (MQ=255)
                                                                                                                cTACGGTGTGGTCTGGACTTTGTGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGTTGAGCCCGGCTGCACTGTc              <  8:176372/135‑1 (MQ=255)
                                                                                                                cTACGGTGTGGTCTGGACTTTGTGGCGCGCTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGTTGAGCCCGGCTGCACTGTc              <  4:108216/135‑1 (MQ=255)
                                                                                                                  aCGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCCGCGAAGCCCCAGTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGTTGAGCCCGGCTGCACTGTCCt            >  8:96678/1‑135 (MQ=255)
                                                                                                                    ggTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTTTTGCTGGCATACCTGTTGAGCCCGGCTGCACTGTCCTAc          >  3:278556/1‑135 (MQ=255)
                                                                                                                            cTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGTTGAGCCCGGCTGCACTGTCCTACGGTTCTAt  >  6:132162/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                            
GTGGCATGGCTGTGGCAAGCGTCGTGGCCTTCATCCTGTGCAAGCGCTTTCCTTCATGGGTACGCCAATGCGGTGAACCCACCGTCGTGGCGCCGGTTGTCAATCAGCCTGACTACGGTGTGGTCTGGACTTTGCGGCGCGTTCTGGCCGACTTCAGCGAAGCCCCATTCTTTGGTAATGAGCTGGCCAGCCTCGGGCTATTGCTGGGTGTGTTGCTGGCATACCTGTTGAGCCCGGCTGCACTGTCCTACGGTTCTAT  >  KT2440/1327293‑1327551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑