Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,589,731 G→T 8.4% G112G (GGC→GGA)  dctD_3 ← C4‑dicarboxylate transport transcriptionalregulatory protein DctD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,589,7310GT 8.4% 130.5 / 3.1 48G112G (GGC→GGA) dctD_3C4‑dicarboxylate transport transcriptionalregulatory protein DctD
Reads supporting (aligned to +/- strand):  ref base G (23/21);  new base T (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

CCGGCGCAGCTGTTGCAGGCCCTGGGACATGCCCAGCAGCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCT  >  KT2440/1589599‑1589861
                                                                                                                                    |                                                                                                                                  
ccGGCGCAGCTGTTGCAGGCCCTGGGACATGCCCAGCAGCGTGCCTTCCAGGCGTGACTTGAGGGCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTg                                                                                                                                    <  5:259627/133‑1 (MQ=255)
 cGGCGCAGCTGTTGCAGGCCCTGGGACATGCCCAGCAGCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGccc                                                                                                                                 <  8:89069/135‑1 (MQ=255)
 cGGCGCAGCTGTTGCAGGCCCTGGGACATGCCCAGCAGCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGGTCTCAAGCACCAGCGGGCGGTTTTGAAGGGCGCGGCGCCGGCTGccc                                                                                                                                 >  5:270007/1‑135 (MQ=255)
  ggCGCAGCTGTTGCAGGCCCTGGGACATGCCCAGCAGCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCa                                                                                                                                >  7:226106/1‑135 (MQ=255)
  ggCGCAGCTGTTGCAGGCCCTGGGACATGCCCAGCAGCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTGCTCAAGCACCAGCTGGCGTTTTTGAAGGGCGCGGCGGAGGCTGCCCa                                                                                                                                >  5:82067/1‑135 (MQ=255)
       aGCTGTTGCAGGCCCTGGGACATGCCCAGCAGCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAg                                                                                                                           >  1:94986/1‑135 (MQ=255)
                aGGCCCTGGGACATGCCCAGCAGCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTgcggc                                                                                                                  <  1:298757/135‑1 (MQ=255)
                    ccTGGGACATGCCCAGCAGCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGa                                                                                                              <  7:139833/135‑1 (MQ=255)
                     cTGGGACATGCCCAGCAGCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGaa                                                                                                             <  8:197269/135‑1 (MQ=255)
                                ccAGCAGCGTGCCTTCCCGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCACGAACCAGCTGGCGTGTTTCAAGGGCGCGGCGCAGGCTGCCCAGCCAGTCGTGCGGCGTGAAGGGGTTTTCCa                                                                                                  >  5:36078/1‑135 (MQ=255)
                                ccAGCAGCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTcc                                                                                                   <  8:204759/134‑1 (MQ=255)
                                 cagcagCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTcc                                                                                                   <  3:131998/133‑1 (MQ=255)
                                 cagcagCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTcc                                                                                                   >  4:131998/1‑133 (MQ=255)
                                 cagcagCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTGCAg                                                                                                 >  5:40334/1‑135 (MQ=255)
                                 cagcagCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGGTTTTCCa                                                                                                  >  8:51923/1‑134 (MQ=255)
                                 cagcagCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGGTTTTCCa                                                                                                  >  2:256059/1‑134 (MQ=255)
                                 cagcagCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTGTTTCAAGGGCGCGGCGCAGGCTGCCCCGCAAGTCGTGCGGCGTGGAGGGGTTTTGCAg                                                                                                 >  7:297863/1‑135 (MQ=255)
                                            ctgccAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGGCTTGCGGCGTGCGGGGGTTTGACCGGCAGGCATAgg                                                                                      >  3:144674/4‑135 (MQ=255)
                                            cTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGCAGCCGGCGGTTATACAGCACCATCTGGCGGTTTTCAAGGGCGCGGCGCAGGCAGCCCCGCCAGTCTTGCGGCGTGAAGGGGTTTTACAGGTAGTAATacg                                                                                      >  7:151781/1‑133 (MQ=255)
                                                cAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGTCGCAGGCTGCCCAGGAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGcc                                                                                    <  7:241541/133‑1 (MQ=255)
                                                cAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGcc                                                                                    <  7:267965/133‑1 (MQ=255)
                                                 aGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCTAGCACCAGCTGGCGGTTTTCAAGGGCGCGGCGCAGGCTGCCCCGCACGGCTTGCGGCGTGAAGGGTTTTTCCCGGTAGTGATAGGgcccc                                                                                 >  7:280446/1‑134 (MQ=255)
                                                    cGTGACATGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGGTTTTCCAGGAAGTCATAGGCCCCGCTg                                                                              <  5:203470/135‑1 (MQ=255)
                                                         cTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTcc                                                                                                   <  7:840/109‑1 (MQ=255)
                                                         cTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTcc                                                                                                   >  8:840/1‑109 (MQ=255)
                                                           tGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTTCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATgg                                                                       >  6:133406/1‑135 (MQ=255)
                                                           tGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTTCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATgg                                                                       >  6:138383/1‑135 (MQ=255)
                                                           tGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATgg                                                                       >  2:177661/1‑135 (MQ=255)
                                                           tGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATgg                                                                       >  7:38249/1‑135 (MQ=255)
                                                           tGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATgg                                                                       >  4:275707/1‑135 (MQ=255)
                                                           tGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATgg                                                                       >  8:262294/1‑135 (MQ=255)
                                                           tGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCGTGCGGCGTGAAGGGGTTTTCCAGGAAGTGCTAGGGCCCGCTGCGCaatg                                                                       >  4:49195/1‑132 (MQ=255)
                                                                   gCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGGTTTTCCTGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTAcc                                                               <  6:57323/135‑1 (MQ=255)
                                                                     cTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGc                                                             <  7:95676/135‑1 (MQ=255)
                                                                     cTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGc                                                             <  2:94986/135‑1 (MQ=255)
                                                                     cTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGc                                                             <  1:130312/135‑1 (MQ=255)
                                                                                gCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGCCATCGGTACAt                                                  <  6:34157/135‑1 (MQ=255)
                                                                                     cggTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGCCATCGGTACATCACCg                                             >  7:84219/1‑135 (MQ=255)
                                                                                              aGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTc                                    <  6:270007/135‑1 (MQ=255)
                                                                                               gCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTca                                   <  5:63177/135‑1 (MQ=255)
                                                                                                         gCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGCCATCGGTACATCACCGTGACGGGTCAGTAGAATTAc                         <  8:151781/135‑1 (MQ=255)
                                                                                                          cGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTAc                         <  4:172587/134‑1 (MQ=255)
                                                                                                          cGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTAc                         <  3:9380/134‑1 (MQ=255)
                                                                                                                tCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAgg                  >  8:184544/1‑135 (MQ=255)
                                                                                                                       cgcggcgcAGGCTGCCCAGCAAGGCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGt           <  4:97038/135‑1 (MQ=255)
                                                                                                                          ggcgcAGGCTGCCCAGCAAGTCTTGCGGCGTGAATGGTTTTTCCAGGAAGTCATATGCCCCGCTGCGCATGGCTTCTACCGCCATCCGTCCATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGCGGTCGt        >  2:37027/1‑135 (MQ=39)
                                                                                                                            cgcAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGCCATCGGTACATCACCGTGCCCGGTCAGCAGCATTACGGGCAGGTCGGGGTCGTTc      >  3:96386/1‑135 (MQ=255)
                                                                                                                                ggCTTCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCt  <  5:138383/135‑1 (MQ=255)
                                                                                                                                ggCTTCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCt  <  5:133406/135‑1 (MQ=255)
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CCGGCGCAGCTGTTGCAGGCCCTGGGACATGCCCAGCAGCGTGCCTTCCAGGCGTGACTTGAGGTCGGCCTGCTCGTGGAGCCGGCGGTTCTCAAGCACCAGCTGGCGTTTTTCAAGGGCGCGGCGCAGGCTGCCCAGCAAGTCTTGCGGCGTGAAGGGTTTTTCCAGGAAGTCATAGGCCCCGCTGCGCATGGCTTCTACCGCCATCGGTACATCACCGTGACCGGTCAGCAGAATTACGGGCAGGTCGGGGTCGTTCGCCT  >  KT2440/1589599‑1589861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑