Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,677,216 G→A 7.4% V247I (GTC→ATC)  namA_2 → NADPH dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,677,2160GA 7.4% 171.0 / 4.2 54V247I (GTC→ATC) namA_2NADPH dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (23/27);  new base A (2/2);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

TTCGAGAATCGAATCCGCCTGACCCTGCAGGTGGTGGAGGCCGTGCGCAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCG  >  KT2440/1677084‑1677343
                                                                                                                                    |                                                                                                                               
gtCGAGAATCGAATCCGCCTGACCCTGCAGGTGGTGGAGGCCGTGCGCAAGGGCGGGCCGGCCGAAGTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTc                                                                                                                               <  6:5487/134‑1 (MQ=255)
    agaATCGAATCCGCCTGACCCTGCAGGTGGTGGAGGCCGTGCGCAAGGTCTGGCCGGCCGTATTGCCGCTGTTCGGGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAAc                                                                                                                           <  2:278766/135‑1 (MQ=255)
           aatcCGCCTGACCCTGCAGGTGGTGGAGGCCGTGCGCAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCtcg                                                                                                                    <  7:174889/135‑1 (MQ=255)
                       ccTGCAGGTGGTGGAGGCCGTGCGCAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGt                                                                                                        <  2:33800/135‑1 (MQ=255)
                       ccTGCAGGTGGTGGAGGCCGTGCGCAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGt                                                                                                        <  5:117121/135‑1 (MQ=255)
                             ggtggtGGAGGCCGTGCGCAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCTGATGACACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTggg                                                                                                  >  7:180244/1‑135 (MQ=255)
                             ggtggtGGAGGCCGTGCGCAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTggg                                                                                                  >  6:17160/1‑135 (MQ=255)
                                ggtggAGGCCGTGCGCAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGgtgt                                                                                               <  5:101143/135‑1 (MQ=255)
                                 gtggAGGCCGTGCGCAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCTGGTGCTGGGTGTc                                                                                              <  7:187116/135‑1 (MQ=255)
                                 gtggAGGCCGGGCGCAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAATCGGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTc                                                                                              <  1:287208/135‑1 (MQ=255)
                                   ggAGGCAGTGCGCAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGCGTGCTGGGTGTCGa                                                                                            >  5:246885/1‑135 (MQ=255)
                                     aGGCCGTGCGCAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGAcc                                                                                          <  4:297399/135‑1 (MQ=255)
                                      ggCCGTGCGCAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCt                                                                                         >  8:90729/1‑135 (MQ=255)
                                          gTGCGCATGGTCTGGCGGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCTACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCGCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATc                                                                                     <  2:110903/135‑1 (MQ=255)
                                          gTGCGCAAGGTGTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGAt                                                                                      >  8:253156/1‑134 (MQ=255)
                                          gTGCGCAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATc                                                                                     <  5:179410/135‑1 (MQ=255)
                                           tGCGCAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCg                                                                                    >  2:141453/1‑135 (MQ=255)
                                             cgcAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGAt                                                                                  <  5:259814/135‑1 (MQ=255)
                                              gcAAGGTCTGGCCGGCCGAATTTCCGCTGTTCGTGCGGGTATCGCCGCCTGCCTGGGTCGAGGACGGCTGGACCCCGGATGCAACCGTCGAACTGGCTCGTCGCCTGCGGGGGCTGGGTGTCGACCTGAGCGAtg                                                                                 >  2:124081/1‑135 (MQ=255)
                                               cAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGCCTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGACCTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGAtga                                                                                >  2:232973/1‑134 (MQ=255)
                                               cAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGAtgt                                                                                <  6:129620/135‑1 (MQ=255)
                                                  ggTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGtc                                                                             <  4:24050/135‑1 (MQ=255)
                                                  ggTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGtc                                                                             <  1:11041/135‑1 (MQ=255)
                                                  ggTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGtc                                                                             <  6:213693/135‑1 (MQ=255)
                                                  ggTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGtc                                                                             <  1:141453/135‑1 (MQ=255)
                                                          cggccgAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTGGAGGACGGCTGGAACCCGGATGAAACCGTCGATCTGGCTCGTCGCCGGGGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCgggg                                                                     >  7:197881/1‑133 (MQ=255)
                                                          cggccgAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCg                                                                                             <  4:210442/111‑1 (MQ=255)
                                                          cggccgAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCg                                                                                             >  3:210442/1‑111 (MQ=255)
                                                          cggccgAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGCCCTGATCGATGTGTCCTCCggtg                                                                     >  7:129180/1‑135 (MQ=255)
                                                           ggccgAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGCCTGGGTCGCGGACGGCTGGAACCCCGATGAAACCGGCGCACTGGCTCGGCGCCTGCGGGTGCGGGGTGTCGCCCGGAGCGATTGGTCCGCCggtgg                                                                    >  2:287448/1‑135 (MQ=255)
                                                           ggccgAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGCCTGGGTCGAGGACGGCTGGAACCCGGATGAAGCCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCggtg                                                                     >  1:185024/1‑134 (MQ=255)
                                                           ggccgAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGCACCCGGATGAAACCGTCGAACTGGCTCGTCGCGTGCGGGTGCTGGGTGGCGACCTGAGCGATGGGTCCTCCggtgg                                                                    >  7:109903/1‑135 (MQ=255)
                                                           ggccgAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACAGGCTCGTCGCCTGCGGGTGCTGTGTGTCGACCTGATCGATGTGTCCTCCggtg                                                                     >  2:102823/1‑134 (MQ=255)
                                                               gAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTAcc                                                                >  4:245998/1‑135 (MQ=255)
                                                                 aTTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTc                                                              >  1:37035/1‑135 (MQ=255)
                                                                     ccGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGt                                                           <  3:12768/134‑1 (MQ=255)
                                                                     ccGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGt                                                           <  5:85555/134‑1 (MQ=255)
                                                                                gCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCATCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAAt                                               >  3:213661/1‑135 (MQ=255)
                                                                                gCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCATCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAAt                                               >  3:217600/1‑135 (MQ=255)
                                                                                  gggTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTc                                             >  1:33638/1‑135 (MQ=255)
                                                                                  gggTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTc                                             <  1:232973/135‑1 (MQ=255)
                                                                                              ctgactgGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGGGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTg                                 <  5:17160/135‑1 (MQ=255)
                                                                                                 actgGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCt                              >  1:112338/1‑135 (MQ=255)
                                                                                                     ggTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCa                          <  3:245044/135‑1 (MQ=255)
                                                                                                     ggTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGAGGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCa                          <  4:36/135‑1 (MQ=255)
                                                                                                       gcGAGGACGGCGGGAAGCCGGATGAAACCGTCGAACTGGCTCGGCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGGGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGa                        <  1:124081/134‑1 (MQ=255)
                                                                                                             aCGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCt                  >  7:28434/1‑135 (MQ=255)
                                                                                                              cGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCtt                 >  2:13270/1‑135 (MQ=255)
                                                                                                                    gAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGgt                                                                                                 >  4:232108/1‑49 (MQ=255)
                                                                                                                    gAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGgt                                                                                                 <  3:232108/49‑1 (MQ=255)
                                                                                                                    gAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCaga           >  1:250920/1‑135 (MQ=255)
                                                                                                                          ggATGAAACCATCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGt     <  4:217600/135‑1 (MQ=255)
                                                                                                                          ggATGAAACCATCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGt     <  4:213661/135‑1 (MQ=255)
                                                                                                                             tGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTgcg  <  7:253156/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                               
TTCGAGAATCGAATCCGCCTGACCCTGCAGGTGGTGGAGGCCGTGCGCAAGGTCTGGCCGGCCGAATTGCCGCTGTTCGTGCGGGTATCGGCGACTGACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCG  >  KT2440/1677084‑1677343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑